Milan Gavrilović, Marina D. Soković, Milan Stanković, Petar D. Marin, Zora Dajić Stevanović, Peđa Janaćković


The antimicrobial and antioxidative activities of diethyl ether, 80% methanol and 50% acetone extracts of the leaves of three Amphoricarpos taxa: A. neumayerianus, A. autariatus ssp. autariatus and A. autariatus ssp. bertisceus (Asteraceae) from the Balkan Peninsula were investigated. The antimicrobial activity was determined by the broth microdilution assay against eight bacterial and eight fungal species. The in vitro antioxidative activity was assessed by the DPPH assay. The total phenolic and flavonoid contents were also determined. The most sensitive bacterial species were Bacillus cereus and Staphylococcus aureus. The best antibacterial potential was obtained for the methanol extract of A. neumayerianus, while the diethyl ether extract of this species showed the lowest effect. In general, the tested extracts showed higher activity than the commercial antibiotics streptomycin and ampicillin. Also, all micromycetes were sensitive to the tested extracts. The most sensitive was Trichoderma viride. The highest and lowest antifungal effect was determined in A. a. ssp. autariatus for the diethyl ether and acetone extracts, respectively. The highest total phenolic and flavonoid contents were determined in the methanol extract of A. a. autariatus. The best antioxidative activity was shown by the methanol extract of A. a. ssp. autariatus as comparing to matching extracts from the other two taxa.

DOI: 10.2298/ABS160112068G

Key words: Amphoricarpos; Asteraceae; leaf extracts; antimicrobial activity; antioxidative potential

Received: January 12, 2016; Revised: February 9, 2016; Accepted: February 9, 2016; Published online: August 2, 2016

How to cite this article: Gavrilović M, Soković MD, Stanković M, Marin P D., Dajić-Stevanović Z, Janaćković P. Antimicrobial and antioxidative activity of various leaf extracts of Amphoricarpos vis. (Asteraceae) taxa. Arch Biol Sci. 2016;68(4):803-10.

Full Text:



Taleb-Contini HS, Schorr K, Da Costa BF, Camilo D, de Oliveira R. Detection of flavonoids in glandular trichomes of Chromolaena Species (Eupatorieae, Asteraceae) by reversed-phase high-performance liquid chromatography. Braz J Pharm Sci. 2007;43:2.

Fritz E. and Saukel J. Anatomy of subterranean organs of medicinally used Cardueae and related species and its value for discrimination. Sci Pharm. 2011;79:157-74.

Baba Aissa F. Flore d’Algérie et du Maghreb. Substances végétales d’Afrique, d’orient et d’occident. Encyclopédie des plantes utiles. Rouiba (Algeria): EDAS, Librairie modern; 1999.

Barrero AF, Herrador MM, Arteaga P, Cabrera E, Rodriguez-Garcia I, Garcia-Moreno M, Gravalos DG. Cytotoxic Activity of Flavonoids from Carthamus arborescens, Ononis natrix ssp. ramosissima and Centaurea malacitana. Fitoterapia. 1997;68:281-3.

Font Quer P. Plantas Medicinales: El Dioscorides Renovado. 15th ed. Madrid: Labor; 1995. 1033 p.

Farrag NM, Abd El Aziz EM, El-Domiaty MM, El Shafea AM. Phytochemical Investigation of Centaurea araneosa Growing in Egypt. J Pharm Sci. 1993;2:29-45.

Djordjević I, Vajs V, Bulatović V, Menković N, Tešević V, Macura S, Janaćković P, Milosavljević S. Guaianolides from two Subspecies of Amphoricarpos neumayeri from Montenegro. Phytochemistry. 2004;65:2337-45.

Djordjević I, Jadranin M, Vajs V, Menković N, Tešević V, Macura S, Milosavljević S. Further Guaianolides from Amphoricarpos neumayeris ssp. murbeckii from Montenegro. Z Naturforsch. 2006;61b:1437-42.

Vallejo JR, Pardo-de-Santayana M, Peral D, Carrasco MC, López D. Uso medicinal de Atractylis gummifera L. en Guadiana del Caudillo (Badajoz, Espana). Toxicidad y especies afines. Rev Fitoterapia. 2008;8:161-9.

Atrrog BAA, Natic M, Tosti T, Opsenica Milojković D, Djordjević I, Tešević V, Jadranin M, Milosavljević S, Lazić M, Radulović S, Tešić Z. Lipophilicity of some Guaianolides isolated from two Endemic Subspecies of Amphoricarpos neumayeri (Asteraceae) from Montenegro. Biomed Chromatogr. 2008;23:250-6.

Vallejo JR, Peral D, Gemio P, Carrasco MC, Heinrich M, Pardo-de-Santayana M. Atractylis gummifera and Centaurea ornata in the Province of Badajoz (Extremadura, Spain) Ethnopharmacological Importance and Toxicological risk. J Ethnopharmacol. 2009;126:366-70.

Jadranin M, Djordjević I, Tešević V, Vajs V, Menković N, Soković M, Glamočlija J, Milosavljević S. Sesquiterpene lactones of Amphoricarpos autariatus ssp. autariatus from Montenegro - antifungal leaf - surface constituents. Rec Nat Prod. 2013;7:234-8.

Susanna A, Garcia-Jacas N. Cardueae (Carduoideae). In: Funk A.V, Susanna A, Stuessy FT, Bayer JR, editors. Systematics, Evolution and Biogeography of Compositae. Vienna (Austria): IAPT; 2009. 293-313.

Caković D, Stešević D, Schönswetter P, Frajman B. How many taxa? Spatiotemporal evolution and taxonomy of Amphoricarpos (Asteraceae, Carduoideae) on the Balkan Peninsula. Org Divers Evol. 2015;15:429-45.

Cvetković M, Djordjević I, Jadranin M, Vajs V, Vučković I, Menković N, Milosavljević S, Tešević V. Further Amphoricarpolides from the surface extracts of Amphoricarpos Complex from Montenegro. Chem Biodivers. 2014;11:1428-37.

Stanković SM, Radojević DI, Stefanović DO, Topuzović DM, Čomić RLj, Branković RS. Immortelle (Xeranthemum annuum L.) as a Natural Source of Biologically Active Substances. EXCLI J. 2011;10:230-9.

Blečić V. and Mayer E. Die europäischen Sippen der Gattung Amphoricarpos Visiani. Phyton (Austria). 1967;12:150-8.

Greuter W. The Euro+Med Treatment of Cardueae (Compositae): Generic Concepts and Required New Names. Willdenowia. 2003;33:49-61.

Hanel H, Raether W. A more Sophisticated Method of Determining the Fungicidal Effect of Water-insoluble Preparations with a Cell Harvester, using Miconazole as an Example. Mycoses. 1988;31:148-54.

Espinel-Ingroff A. Comparation of the E-test with the NCCLS M38-P Method for Antifungal Susceptibility Testing of Common and Emerging Pathogenic Filamentous Fungi. J Clin Microbiol. 2001;39:1360-7.

Booth C. Fungal culture media. In: Norris JR, Ribbons DW, editors. Methods in Microbiology. London, New York: Academic Press; 1971. 49-94.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent oxidants and antioxidants. Method Enzymol. 1999;299:152-78.

Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx C, Cazin M, Cazin JC, Bailleul F, Trotin F et al. Phenolic Compounds and Antioxidant Activities of Buckwheat (F. esculentum Moench) Hulls and Flour. J Ethnopharmacol. 2000;72:35-42.

Tekao T, Watanabe N, Yagi I, Sakata K. A Simple Screening Method for Antioxidant and Isolation of Several Antioxidants Produced by Marine Bacteria from Fish and Shellfish. Biosci Biotechnol Biochem. 1994;58:1780-3.

Kumarasamy Y, Byres M, Cox PJ, Jasapars M, Nahar L, Sarker SD. Screening Seeds of some Scottish Plants for Free-radical Scavenging Activity. Phytother Res. 2007;21:615-21.

Katalinić V, Miloš M, Kulišić T, Jukić M. Screening of 70 Medicinal Plant Extracts for Antioxidant Capacity and Total Phenols. Food Chem. 2004;94:550-7.


  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.