Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress

Rabiye Terzi, Neslihan Saruhan Güler, Funda Gül Güven, Asim Kadioglu

Abstract


Paper description:

  • Exogenous alpha lipoic acid (ALA) plays a role in promoting salt tolerance in plants. However, the effects of ALA application on the antioxidant system have not been sufficiently clarified in plants exposed to abiotic stresses.
  • The effects of ALA application on the activities of antioxidant enzymes and membrane damage were investigated in maize seedlings under osmotic stress.
  • ALA application elevated activities of antioxidant enzymes and ameliorated lipid peroxidation.
  • Exposure to ALA improves resistance against osmotic stress by inducing the antioxidant system in maize.


Abstract: Plants are markedly affected by drought stress caused by fluctuations in global climate, reduction in rainfall and a decrease in soil fertility. Therefore, some mechanistic strategies to cope with adverse effects of drought stress are needed. Alpha lipoic acid (ALA), a potent antioxidant molecule, is known to function in abiotic stress tolerance. In the current study, we investigated the ALA-stimulated physiological role in tolerance to osmotic stress induced by polyethylene glycol in two maize (Zea mays L.) cultivars (cv. Helen and cv. Akpinar). Application of ALA increased the leaf water potential of maize cultivars under stressful and stress-free conditions but decreased lipid peroxidation and the hydrogen peroxide (H2O2) content. Additionally, enhanced activity of the antioxidant defense system was observed following ALA application. Exogenous ALA elevated the activities of enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), glutathione reductase (GR) and monodehydroascorbate reductase (MDHAR) under osmotic stress as compared to seedlings not exposed to ALA. Conversely, ascorbate peroxidase (APX) activity was decreased by ALA application in both cultivars. Higher GR and MDHAR activities of both cultivars were simultaneously observed in ALA treatments under osmotic stress. Taken together, the data indicated that exogenous ALA may function in arranging resilience against osmotic stress by reducing oxidative damage through induction of the antioxidant machinery in maize cultivars.

https://doi.org/10.2298/ABS171218011T

Received: December 18, 2017; Revised: February 22, 2018; Accepted: March 20, 2018; Published online: April 4, 2018

How to cite this article: Terzi R, Saruhan Güler N, Güven FG, Kadioglu A. Alpha lipoic acid treatment induces the antioxidant system and ameliorates lipid peroxidation in maize seedlings under osmotic stress. Arch Biol Sci. 2018;70(3):…



Keywords


alpha lipoic acid; antioxidant system; osmotic stress; tolerance; Zea mays

Full Text:

PDF

References


Smirnoff N. Plant resistance to environmental stress. Curr Opin Biotechnol. 1998;9:214-19.

Hasanuzzaman M, Hossain MA, Teixeira da Silva JA, Fujita M. Plant responses and tolerance to abiotic oxidative stress: antioxidant defense is a key factors. In: Bandi V, Shanker AK, Shanker C, Mandapaka M, editors. Crop stress and its management: perspectives and strategies. Berlin, Germany: Springer; 2012. p. 261-316.

Hasanuzzaman M, Alam M, Rahman A, Hasanuzzaman Md, Nahar K, Fujita M. Exogenous proline and glycine betaine mediated upregulation of antioxidant defense and glyoxalase systems provides better protection against salt-induced oxidative stress in two rice (Oryza sativa L.) varieties. Biomed Res Int. 2014;2014:1-17.

Packer L, Witt EH, Tritschler HJ. Alpha-lipoic acid as a biological antioxidant. Free Radic Biol Med. 1995;19:227-50.

Navari-Izzo F, Quartacci MF, Sgherri CLM. Lipoic acid: a unique antioxidant in the detoxification of activated oxygen species. Plant Physiol Biochem. 2001;40:463-70.

Sgherri C, Quartacci MF, Izzo R, Navari-Izzo F. Relation between lipoic acid and cell redox status in wheat grown in excess copper. Plant Physiol Biochem. 2002;40:591-7.

Perez-Lopez U, Robredo A, Lacuesta M, Sgherri C, Mena-Petite A, Navari-Izzo F, Munoz-Rueda A. Lipoic acid and redox status in barley plants subjected to salinity and elevated CO2. Physiol Plant. 2010;139:256-68.

Yildiz M, Akçali N, Terzi H. Proteomic and biochemical responses of canola (Brassica napus L.) exposed to salinity stress and exogenous lipoic acid. J Plant Physiol. 2015;179:90-9.

Gorcek Z, Erdal S. Lipoic acid mitigates oxidative stress and recovers metabolic distortions in salt-stressed wheat seedlings by modulating ion homeostasis, the osmo-regulator level and antioxidant system. J Sci Food Agric. 2015;95:2811-17.

Crutzen PJ, Mosier AR, Smith KA, Winiwarter W. N2O release from agro-biofuel production negates global warming reduction by replacing fossil fuels. Atmos Chem Phys. 2008;8:389-95.

Food and Agriculture Organization of the United Nations. Save and grow in practice maize rice wheat. A guide to sustainable cereal production. Rome: Food and Agriculture Organization of the United Nations; 2016. 109 p.

Pehlivan N, Yesilyurt AM, Durmus N, Karaoglu SA. Trichoderma lixii ID11D seed biopriming mitigates dose dependent salt toxicity in maize. Acta Physiol Plant. 2017;39(3):79.

Terzi R, Kalaycioglu E, Demiralay M, Saglam A, Kadioglu A. Exogenous ascorbic acid mitigates accumulation of abscisic acid, proline and polyamine under osmotic stress in maize leaves. Acta Physiol Plant. 2015;37:43.

Fu J, Huang B. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ Exp Bot. 2001;45:105-14.

Heath RL, Packer L. Photoperoxidation in isolated chloroplast. I. kinetics and stochiometry of fatty acid peroxidation. Arch Biochem Biophys. 1968;125:189-98.

Velikova V, Yordanov I, Edreva A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants, protective role of exogenous polyamines. Plant Sci. 2000;151:59-66.

Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971;44:276-87.

Urbanek H, Kuzniak-Gebarowska E, Herka H. Elicitation of defense responses in bean leaves by Botrytis cinerea polygalacturonase. Acta Physiol Plant. 1991;13:43-50.

Nakano Y, Asada K. Hydrogen peroxide is by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981;22:867-80.

Aebi H. Catalase. In: Bergmeyer H, editor. Methods of enzymatic analysis. Chemie: Weinheim-Verlag; 1983. p. 273-86.

Foyer CH, Halliwell B. Presence of glutathione and glutathione reductase in chloroplast: a proposed role in ascorbic acid metabolism. Planta. 1976;133:21-5.

Hossain MA, Nakano Y, Asada K. Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol. 1984;25:385-95.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.

Farooq M, Basra SMA, Wahid A, Ahmad N, Saleem BA. Improving the drought tolerance in rice (Oryza sativa L.) by exogenous application of salicylic acid. J Agron Crop Sci. 2009;195:237-46.

Mohammadkhani N, Heidari N. Water stress induced by polyethylene glycol 6000 and sodium chloride in two maize cultivars. Pak J Biol Sci. 2008;11:92-7.

Lai QX, Bao ZY, Zhu ZJ, Qian QQ, Mao BZ. Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12- IPT Modified gerbera. J Zhejiang Univ Sci B. 2007;8:458-64.

Liu ZJ, Guo YK, Bai JG. Exogenous hydrogen peroxide changes antioxidant enzyme activity and protects ultrastructure in leaves of two cucumber ecotypes under osmotic stress. J Plant Growth Regul. 2010;29:171-83.

Della Croce C, Bronzetti G, Cini M, Caltavuturo L, Poi G. Protective effect of lipoic acid against hydrogen peroxide in yeast cells. Toxicol in Vitro. 2003;17: 753-9.

Moini H, Packer L, Saris NEL. Antioxidant and prooxidant activities of α-lipoic acid and dihydrolipoic acid. Toxicol Appl Pharm. 2002;182:84-90.

Ksouri R, Megdiche W, Debez A, Falleh H, Grignon C, Abdelly C. Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritime. Plant Physiol Biochem. 2007;45:244-9.

Foyer CH, Noctor G. Ascorbate and glutathione: the heart of the redox hub. Plant Physiol. 2011;155:2-18.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 ARCHIVES OF BIOLOGICAL SCIENCES

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.