Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles

Authors

  • Marko B. Mitrović Department of Radiology and Radiation Hygiene, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobođenja 18, 11000 Belgrade http://orcid.org/0000-0002-0416-0103
  • Nikola R. Tatalović Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-0745-8845
  • Aleksandra L. Nikolić-Kokić Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-1116-2035
  • Olivera F. Ciraj-Bjelac Radiation Protection Department, Vinča Institute of Nuclear Sciences, University of Belgrade, Mike Petrovića Alasa 12-14, 11351 Belgrade http://orcid.org/0000-0001-9577-011X
  • Nikola E. Krstić Department of Radiology and Radiation Hygiene, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobođenja 18, 11000 Belgrade http://orcid.org/0000-0001-7326-6262
  • Zorana S. Oreščanin-Dušić Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-2772-0134
  • Dragana Ž. Krstić Department of Physics, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac http://orcid.org/0000-0002-3517-9210
  • Zoran M. Jovanović Department of Physics, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac http://orcid.org/0000-0002-0898-8960
  • Duško P. Blagojević Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0001-6338-2833
  • Mirjana V. Lazarević-Macanović Department of Radiology and Radiation Hygiene, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobođenja 18, 11000 Belgrade http://orcid.org/0000-0003-0668-184X

Keywords:

computed tomography, oxidative stress, antioxidant enzymes, testicles, rabbit

Abstract

Paper description:

  • There is no literature data on the influence of diagnostic doses of x-radiation during computed tomography (CT) on the oxidative status in testicles.
  • Our results showed that CT provoked a response at the level of antioxidant enzymes in rabbit testicles which was time- and dose-dependent, with a dose threshold of about 29.2 mGy, and predominantly under the influence of the applied current.
  • Our results present novel information about the influence of CT on the cellular antioxidant process.

Abstract: In recent years, computed tomography (CT) has become very common in veterinary medicine. It is well known that testicles are organs with high radiosensitivity and their function can be impaired even after exposure to low radiation doses. In this work, we calculated the absorbed radiation doses after CT was performed with different voltage/current levels and correlated it with the activity of antioxidant enzymes in rabbit testicles. Two hours after CT, the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) were increased in the testicles of animals that received an absorbed dose of 29.2 mGy. The same changes, along with elevated glutathione reductase (GR) activity, were observed after 7 days in animals that received the highest absorbed dose (46.3 mGy). It would appear that absorbed doses above 27.8 mGy provoked the antioxidant reaction but the time scale of the reaction was dose-dependent. Examination of the obtained results revealed that the main denominator of CT influence was a higher current. Our results suggest that CT influences the antioxidant status in rabbit testicles. The changes in antioxidant enzyme activities were dose- and time-dependent and influenced by the applied current.

https://doi.org/10.2298/ABS180413029M

Received: April 13, 2018; Revised: May 23, 2018; Accepted: June 12, 2018; Published online: June 15, 2018

How to cite this article: Mitrović MB, Tatalović NR, Nikolić-Kokić AL, Ciraj-Bjelac OF, Krstić NE, Oreščanin-Dušić ZS, Krstić DŽ, Jovanović ZM, Blagojević DP, Lazarević-Macanović MV. Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles. Arch Biol Sci. 2018;70(4):675-80.

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Author Biography

Aleksandra L. Nikolić-Kokić, Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade


References

Aitken RJ, Roman SD. Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longevity. 2008;1(1):15-24.

Bas H, Kalender S. Antioxidant Status, Lipid Peroxidation and Testis-histoarchitecture Induced by Lead Nitrate and Mercury Chloride in Male Rats. Braz Arch Biol Technol. 2016;59:e16160151.

Acharya UR, Mishra M, Patro J, Panda MK. Effect of vitamins C and E on spermatogenesis in mice exposed to cadmium. Reprod Toxicol. 2008;25:84-8.

Peltola V, Parvinen M, Huhtaniemi I, Kulmala J. Comparison of Effects of 0.5 and 0.3 Gy X-Irradiation on Lipid Peroxidation and Antioxidant Enzyme Function in Rat Testis and Liver. EJBMSR. 1993; 14(4):267-74.

National Research Council (U.S.) Committee to Assess Health Risks from Exposure to Low Level of Ionizing Radiation. Health risks from exposure to low levels of ionizing radiation : BEIR VII, Phase 2. Washington, D.C.: National Academies Press; 2006. 406 p.

Howell SJ, Shalet SM. Spermatogenesis after cancer treatment: damage and recovery. J Natl Cancer Inst Monogr. 2005;34:12-7.

Kovacs GT, Stern K. Reproductive aspect of cancer treatment: an update. Med J Aust. 1999;170:495-7.

Suntharalingam N. Basic Radiobiology. In: Podgorsak EB, editor. Review of Radiation Oncology Physics, A Handbook for Teachers and Students. Vienna: International Atomic Energy Agency; 2003. p. 397-412.

Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG. Improving Bioscience Research Reporting: The ARRIVE Guidelines for Reporting Animal Research. PLOS Biol. 2010;8(6):e1000412.

Monte Carlo Team. MCNP–a General Monte Carlo N-Particle Transport Code (X-5 Monte Carlo Team, Version 5). Vol. 1: Overview and Theory. Los Alamos, NM: Los Alamos National Laboratory; 2003. LA- UR-03-1987.

Mirsa HP, Fridovic I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972;247(10):3170-5.

Beutler E. Catalase. In: Beutler E, editor. Red Cell Metabolism, A Manual of Biochemical Methods. New York: Grune and Stratton Inc; 1982. p. 105-6.

Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70(1):158-69.

Glatzle D, Vuillemier JP, Weber F, Decker K. Glutathione reductase test with whole blood, a convenient procedure for the assessment of riboflavin status in humans. Experientia. 1974;30(6):665-7.

Lowry OH, Rosenbrough NJ, Farr A, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265-75.

Kowaltowski AJ, Seetharaman S, Paucek P, Garlid KD. Bioenergetic consequences of opening the ATP-sensitive K channel of heart mitochondria. Am J Physiol Heart Circ Physiol. 2001;280:649-57.

Murata M, Akao M, O’Rourke B, Marbán E. Mitochondrial ATP-sensitive potassium channels attenuate matrix Ca(2+) overload during simulated ischemia and reperfusion. Possible mechanism of cardioprotection. Circ Res. 2001;89:891-8.

Zaugg M, Lucchinetti E, Spahn DR, Pasch T, Schaub MC. Volatile anesthetics mimic cardiac preconditioning by priming the activation of mitochondrial K(ATP) channels via multiple signaling pathways. Anesthesiology. 2002;97:4-14.

Zaugg M, Lucchinetti E, Spahn DR, Pasch T, Garcia C, Schaub MC. Differential Effects of Anesthetics on Mitochondrial K(ATP) Channel Activity and Cardiomyocyte Protection. Anesthesiology. 2002;97:15-23.

Mitrović BM, Ciraj-Bjelac FO, Oreščanin-Dušić SZ, Blagojević PD, Nikolić-Kokić LA, Tatalović RN, Krstić EN, Lazarević-Macanović VM. Influence of radiation dose in computed tomography on antioxidant enzyme activity in rabbit erythrocytes. Nucl Technol Radiat. 2017;32(4):342-9.

Sanderman TF. The effects of X-irradiation on male human fertility. Br J Radiol. 1966;39:901-7.

Lahdetie J, Parvinen M. Meiotic micronuclei induced by x-rays in early spermatids of the rat. Mutat Res. 1981;81:103-15.

Halliwel B, Gutteridge JMC. Oxygen toxicity, oxygen radicals, transition metals and desease. Biochem J. 1984;219:1-14.

Halliwel B, Clement MV, Long LH. Hydrogen peroxide in the human body. FEBS Lett. 2000;486:10-3.

Korać B, Blagojević D, Saičić ZS, Buzadžić B, Spasić MB, Petrović VM. Effects of acute x-ray irradiation on antioxidative defence in the rats chronically exposed to cold. Arch Biol Sci. 1993;46(3-4):87-93.

Petrović VM, Saičić ZS, Spasić MB, Radojičić R, Buzadžić B. Hormones and antioxidant defence. In: Nygaard OF, Lipton AC, editors. Anticarcinogenesis and Radiation Protection 2. New York: Plenum Press; 1991. p. 405-16.

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Published

2018-12-04

How to Cite

1.
Mitrović MB, Tatalović NR, Nikolić-Kokić AL, Ciraj-Bjelac OF, Krstić NE, Oreščanin-Dušić ZS, Krstić D Ž., Jovanović ZM, Blagojević DP, Lazarević-Macanović MV. Influence of absorbed radiation dose following computed tomography on the antioxidative status in rabbit testicles. Arch Biol Sci [Internet]. 2018Dec.4 [cited 2024Apr.16];70(4):675-80. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2841

Issue

Vol. 70 No. 4 (2018)

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Articles

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