Cytotoxicity and genotoxicity of iron oxide nanoparticles: An in vitro biosafety study

Authors

  • Erdal Sonmez 1. K. K. Education Faculty, Department of Physics, Atatürk University, Erzurum 2. Graduate School of Natural and Applied Sciences, Department of Nanoscience & Nanoengineering, Advanced Materials Research Laboratory, Atatürk University, Erzurum,
  • Elanur Aydin Faculty of Science, Erzurum Technical University, Erzurum
  • Hasan Turkez 1. Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum 2. Department of Pharmacy, "G. D'Annunzio" University, Chieti, Italy
  • Elvan Özbek Sakarya University, Faculty of Medicine, Sakarya
  • Basak Togar Atatürk University, Faculty of Science, Erzurum
  • Kadem Meral 1. Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum 2. Atatürk University, Faculty of Science, Department of Chemistry, Erzurum
  • Damla Çetin Atatürk University, Faculty Medical Pharmacology, Erzurum
  • Ivana Cacciatore Department of Pharmacy, "G. D'Annunzio" University, Chieti
  • Antonio Di Stefano Department of Pharmacy, "G. D'Annunzio" University, Chieti

Abstract

With the development of nanotechnology and the wide use of iron oxide nanoparticles, it has become necessary to assess the potential adverse biological effects of magnetite. This study investigated the cytotoxicity, genotoxicity and oxidative damage of different concentrations of magnetite (0 to 1000 mg/L) in human whole blood cultures. After supplementation of magnetite, the blood samples were incubated for 72 h. Cell viability was assessed by the 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays. The total antioxidant capacity (TAC) and total oxidant status (TOS) were determined to evaluate the dose-dependent effects of magnetite on the oxidant/antioxidant balance and to evaluate the potential oxidative injury due to increased oxidative stress. Genotoxicity was estimated by by the sister chromatid exchange (SCE), micronuclei (MN) and chromosome aberration (CA) assays and determination of 8-oxo-2-deoxyguanosine (8-OH-dG) levels. The results of MTT and LDH assays showed that the higher concentrations of magnetite (100, 150, 300, 500 and 1000 mg/L) decreased cell viability. Concentrations of magnetite higher than 10 mg/L increased TOS levels and decreased TAC levels in human blood cells. Increasing concentrations of magnetite caused significant increases in MN, SCE and CA rates and 8-OH-dG levels. The obtained results showed that magnetite exerted dose-dependent effects on oxidative damage, genotoxicity and cytotoxicity in human blood cells.

Downloads

Download data is not yet available.

Author Biographies

Elanur Aydin, Faculty of Science, Erzurum Technical University, Erzurum

Department of Molecular Biology and Genetics

Elvan Özbek, Sakarya University, Faculty of Medicine, Sakarya

Department of Histology and Embryology

Basak Togar, Atatürk University, Faculty of Science, Erzurum

Department of Biology

Downloads

Published

2016-06-24

How to Cite

1.
Sonmez E, Aydin E, Turkez H, Özbek E, Togar B, Meral K, Çetin D, Cacciatore I, Di Stefano A. Cytotoxicity and genotoxicity of iron oxide nanoparticles: An in vitro biosafety study. Arch Biol Sci [Internet]. 2016Jun.24 [cited 2024Mar.29];68(1):41-50. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/741

Issue

Section

Articles