@article{Bakhsh_Anayol_Khabbazi_Karakoç_Sancak_Özcan_2016, title={DEVELOPMENT OF INSECT-RESISTANT COTTON LINES WITH TARGETED EXPRESSION OF INSECTICIDAL GENE}, volume={68}, url={https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1249}, abstractNote={<p>In order to address biosafety concerns regarding the constitutive expression of foreign genes in crops, we applied a strategy aimed at confining foreign gene expression in insect wounding sites of cotton. For this purpose, a plant expression construct was designed by cloning the AoPR1 promoter (pathogenesis-related protein gene isolated from <em>Asparagus officinalis</em>) upstream from the insecticidal gene c<em>ry1Ac</em>. The Turkish cotton cultivar cv. STN-468 was transformed using the <em>Agrobacterium tumefaciens </em>strain LBA4404 containing the recombinant binary vector pRD400 harboring c<em>ry1Ac</em> under a wound-inducible promoter. The neomycin phosphotransferase (<em>nptII</em>) gene was used as a selectable marker at a concentration of 100 mg/L. The primary transformants were analyzed for T-DNA integration and expression using standard molecular approaches. The efficacy of insecticidal gene control of the AoPR1 promoter was investigated using leaf bioassays with 2<sup>nd</sup> instar larvae of <em>Helicoverpa armigera</em> and <em>Spodoptera littoralis</em>. Positive primary transformants from T<sub>0 </sub>progeny were further raised under greenhouse conditions to obtain progeny (T<sub>1</sub>). The introduced gene was properly inherited and expressed in T<sub>1</sub> progeny. The mechanical wounding of plants resulted in increased cry1Ac protein levels during 0-48 h of the wounding period. The transgenic lines exhibited appreciable levels of resistance against targeted insect pests in the leaf bioassays. The use of a wound-inducible promoter to drive insecticidal gene expression is a valuable insect resistant management strategy as gene expression will remain limited to the insect biting sites of plant and crop, food and environmental concerns can be minimized.</p><p><strong>DOI:</strong> <a href="http://doiserbia.nb.rs/Article.aspx?id=0354-46641600063B">10.2298/ABS151012063B</a></p><div><p><strong>Key words</strong>:<strong> </strong>cotton modification; cry toxin; confined expression; insecticidal gene</p><div><p><strong>Accepted:</strong> October 12, 2015; <strong>Revised:</strong> October 27, 2015; <strong>Accepted:</strong> November 4, 2015; <strong>Published online:</strong> August 2, 2016</p><p><strong>How to cite this article:</strong> Bakhsh A, Anayol E, Khabbazi SD, Karakoç ÖC, Sancak C, Özcan S. Development of insect-resistant cotton lines with targeted expression of insecticidal gene. Arch Biol Sci. 2016;68(4):773-80.</p></div></div>}, number={4}, journal={Archives of Biological Sciences}, author={Bakhsh, Allah and Anayol, Emine and Khabbazi, Saber Delpasand and Karakoç, Ömer Cem and Sancak, Cengiz and Özcan, Sebahattin}, year={2016}, month={Nov.}, pages={773–780} }