@article{Liu_Ma_Gai_Sun_Wang_He_Gao_2018, title={Analysis of putative sclerotia maturation-related gene expression in Rhizoctonia solani AG1-IA}, volume={70}, url={https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2526}, abstractNote={<div><p><strong>Paper description: </strong></p><ul><li>A cDNA library of <em>Rhizoctonia solani</em> AG1-IA, a major soil-borne fungal pathogen of maize,<em> </em>was constructed and qRT-PCR analysis was performed to determine the expression of twelve <em>R. solani</em> AG1-IA genes during three stages of sclerotial formation (mycelium, initiation of sclerotium and maturation of sclerotium).</li><li>The results of our study provide new findings related to the methods of prevention and control of disease caused by <em>R. solani</em> AG1-IA.</li></ul><p><strong><br /></strong></p><p><strong>Abstract</strong><strong>:</strong><em>Rhizoctonia solani</em> AG1-IA (<em>R. solani</em> AG1-IA) is a major soil-borne fungal pathogen of maize that causes significant yield losses in all maize-growing regions worldwide. The sclerotium produced by <em>R. solani</em> AG1-IA can overwinter in grass roots or diseased plants and infect crops the following year. The molecular mechanism underlying sclerotium formation in <em>R. solani </em>is poorly understood. In this study, we constructed the cDNA library of the <em>R. solani</em> AG1-IA pathogenic strain WF-9, from which 329 high-quality expressed sequence tags (ESTs) were obtained. Of the 250 clustered unigenes, 12 genes were selected for further expression analysis during the three stages of sclerotial growth (mycelium, initiation of sclerotium, and maturation of sclerotium). The results of expression analysis support the previously suggested roles of chitin synthase D and exo-beta-1,3-glucanase in facilitating sclerotial growth through preservation of water content and energy. In addition, cytochrome P450, NADPH oxidase, catalase (CAT), acyl-CoA oxidase 1 (ACOX1), mitogen-activated protein kinase (MAPK), mitogen-activated protein kinase HOG1 (HOG 1), and the G-protein α subunit play important roles in balancing reactive oxygen species (ROS) levels during sclerotial development. The findings of this study can help understand the molecular mechanism of sclerotial development further.</p><p><a href="https://doi.org/10.2298/ABS180106026L">https://doi.org/10.2298/ABS180106026L</a></p><p><strong>Received: </strong>January 6, 2018;<strong> Revised: </strong>May 1, 2018; <strong>Accepted:</strong> May 4, 2018; <strong>Published online:</strong> Jun 14, 2018</p><p><strong>How to cite this article: </strong>Liu B, Ma Z, Gai X, Sun Y, Wang Y, He S, Gao Z. Analysis of putative sclerotia maturation-related gene expression in <em>Rhizoctonia solani</em> AG1-IA. Arch Biol Sci. 2018;70(4):647-53.</p></div>}, number={4}, journal={Archives of Biological Sciences}, author={Liu, Bo and Ma, Zhoujie and Gai, Xiaotong and Sun, Yanqiu and Wang, Yanfeng and He, Shidao and Gao, Zenggui}, year={2018}, month={Dec.}, pages={647–653} }