@article{Liu_Ji_Chen_Lin_Wei_Zhu_Lv_2017, title={Overexpression of the mulberry latex gene MaMLX-Q1 enhances defense against <i>Plutella xylostella</i> in <i>Arabidopsis thaliana</i>}, volume={69}, url={https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/338}, abstractNote={<p>Purified mulberry latex chitinase (MLX) has a role in defense against some lepidopteran<em> </em>insects. In this study, a full length chitinase gene, <em>MaMLX-Q1, </em>of 1405 bp with a 1140 bp open reading frame, was obtained from mulberry leaves by the degenerate primers and rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR) procedure. The gene encoded a mature protein with the predicted molecular mass of 39.38 kDa and an isoelectric point (pI) of 6.43; it contained two chitin-binding domains and a hydrolase family 19 chitinase domain. Sequence alignment and phylogenetic analysis grouped it in the class I chitinase protein group. Heterogeneous expression of this MaMLX-Q1 in <em>Arabidopsis</em> showed non-visible alterations in growth phenotype, except for the higher transcriptional expression of <em>MaMLX-Q1</em> when compared to that of wild-type <em>Arabidopsis</em>. This ectopic <em>MaMLX-Q1</em> exhibited toxicity to the growth and development of <em>Plutella xylostella</em> larvae, causing significantly lower weight gain and higher mortality. These results indicate an application of <em>MaMLX-Q1</em> as an insecticide for plant protection.</p><p align="left"><a href="https://doi.org/10.2298/ABS160315097L">https://doi.org/10.2298/ABS160315097L</a></p><div><p><strong>Received:</strong> March 15, 2016; <strong>Revised:</strong> April 24, 2016; <strong>Accepted:</strong> May 4, 2016; <strong>Published online:</strong> October 17, 2016</p><p align="left"><strong>How to cite this article:</strong> Liu Y, Ji D, Chen J, Lin T, Wei J, Zhu Y, Lv Z.<strong> </strong>Overexpression of the mulberry latex gene MaMLX-Q1 enhances defense against <em>Plutella xylostella</em> in <em>Arabidopsis thaliana.</em> Arch Biol Sci. 2017;69(2):269-76.</p></div>}, number={2}, journal={Archives of Biological Sciences}, author={Liu, Yan and Ji, Dongfeng and Chen, Jine and Lin, Tianbao and Wei, Jia and Zhu, Yan and Lv, Zhiqiang}, year={2017}, month={May}, pages={269–276} }