@article{Dragićević_Ćuković_Zdravković-Korać_Simonović_Bogdanović_Todorović_2019, title={Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach}, volume={71}, url={https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4099}, abstractNote={<p><strong>Abstract:</strong> Glutamine synthetase (E.C. 6.3.1.2) is a key enzyme of plant nitrogen metabolism that assimilates ammonia into glutamine. The Arabidopsis thaliana genome encodes one chloroplastic (GLN2) and five cytosolic (GLN1;1 – GLN1;5) isoforms with different expression patterns, kinetic properties, regulation and functions. Physiological roles of different isoforms have been elucidated mainly by studying knockout mutants. However, the role of GLN1;5, which is expressed in dry seeds, remains unknown. To clarifty the function of GLN1;5, we studied a GLN1;5 knockout line (GLN1;5KO) homozygous for T-DNA insertion within the GLN1;5. GLN1;5 deficiency results in a phenotype with slightly delayed bolting and fewer siliques. The dry weight of GLN1;5KO seeds was 73.3% of wild-type (WT) seed weight, with seed length 90.9% of WT seeds. Finally, only 18.33% of the mutant seeds germinated in water within 10 days in comparison to 34.67% of WT seeds. KNO3 strongly stimulated germination of both GLN1;5KO and WT seeds, while germination in the presence of increasing NH4Cl concentrations potentiated the differences between the two genotypes. It can be concluded that GLN1;5 activity supports silique development and grain filling and that it has a role in ammonium reassimilation in the seed, as well as assimilation and/or detoxification of ammonium from the environment.</p><p><a href="https://doi.org/10.2298/ABS190315026D">https://doi.org/10.2298/ABS190315026D</a></p><p><strong>Received:</strong> March 15, 2019<strong>; Accepted:</strong> April 4, 2019; <strong>Published online: </strong>April 10, 2019</p><p><strong>How to cite this article:</strong> Dragićević MB, Ćuković KB, Zdravković-Korać SR, Simonović AD, Bogdanović MD, Todorović SI. Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in <em>Arabidopsis thaliana</em> (L.) with a reverse genetics approach. Arch Biol Sci. 2019;71(3):443-53.</p>}, number={3}, journal={Archives of Biological Sciences}, author={Dragićević, Milan B. and Ćuković, Katarina B. and Zdravković-Korać, Snežana R. and Simonović, Ana D. and Bogdanović, Milica D. and Todorović, Slađana I.}, year={2019}, month={Oct.}, pages={443–453} }