@article{Zhang_Sun_Zou_2020, title={LncRNA NEAT1 exacerbates non-small cell lung cancer by upregulating EIF4G2 via miR-582-5p sponging}, volume={72}, url={https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5098}, abstractNote={<div><p><strong>Paper description:</strong></p><ul><li>lncRNA-long non-coding RNA nuclear enriched abundant transcript 1 (NEAT1) is involved in the initiation and progression of multiple cancers.</li><li>We examined the function of NEAT1 in non-small cell lung cancer (NSCLC); cytobiological and biochemical experiments were performed to study its role in an <em>in vivo</em> mouse model.</li><li>NEAT1 potentiated the malignant potential of NSCLC cells (migration and glycolysis) through the microRNA (miR)-582-5p/eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) subunit axis.</li><li>The NEAT1/miR-582-5p/EIF4G2 axis could serve as a potential diagnostic or prognostic marker in NSCLC treatment.</li></ul><br /><p><strong>Abstract:</strong> In this study, we aimed to elucidate the role of long non-coding RNA nuclear enriched abundant transcript 1 (lncRNA NEAT1) in non-small cell lung cancer (NSCLC). Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to detect the abundance of NEAT1, microRNA-582-5p (miR-582-5p) and eukaryotic translation initiation factor 4 gamma 2 (EIF4G2). Proliferation, apoptosis, metastasis and glycolytic metabolism were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) flow cytometry, transwell assays and fluorescence-based glucose and lactate assay kits. The targets of NEAT1 and miR-582-5p were predicted by the starBase website, and dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were performed to verify these predictions. Western blot analysis was conducted to detect the protein expression of EIF4G2. A xenograft tumor model was built to clarify the role of NEAT1 <em>in vivo</em>. Results showed that NEAT1 interference inhibited proliferation, metastasis and glycolysis, and facilitated the apoptosis of NSCLC cells. MiR-582-5p was a functional target of NEAT1, and the biological influence of NEAT1 intervention on NSCLC cells was alleviated by transfection with anti-miR-582-5p. MiR-582-5p could bind to EIF4G2 messenger RNA (mRNA); it exerted its antitumor role in NSCLC cells by inhibiting EIF4G2. EIF4G2 was regulated by NEAT1/miR-582-5p signaling. NEAT1 accelerated NSCLC tumor growth via the miR-582-5p/EIF4G2 axis <em>in vivo</em>. In conclusion, NEAT1 affected NSCLC by elevating their malignant potential via the miR-582-5p/EIF4G2 axis.</p><p><a href="https://doi.org/10.2298/ABS200218018Z">https://doi.org/10.2298/ABS200218018Z</a></p><p><strong>Received:</strong> February 18, 2020; <strong>Revised:</strong> April 16, 2020; <strong>Accepted:</strong> April 17, 2020; <strong>Published online:</strong> April 23, 2020</p><p><strong>How to cite this article:</strong> Zhang X, Sun Z, Zou Y.LncRNA NEAT1 exacerbates non-small cell lung cancer by upregulating EIF4G2 via miR-582-5p sponging. Arch Biol Sci. 2020;72(2):243-52.</p></div>}, number={2}, journal={Archives of Biological Sciences}, author={Zhang, Xin and Sun, Zhonghua and Zou, Yuepei}, year={2020}, month={Jul.}, pages={243–252} }