@article{Nikolić_Ljujić_Bojović_Mitić_Rajčević_Tešević_Marin_2020, title={Headspace volatiles isolated from twigs of Picea omorika from Serbia}, volume={72}, url={https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5348}, abstractNote={<div><p class="normal"><strong>Paper description:</strong></p><ul><li>The headspace method (HS) enables us to study the composition of volatile compounds by using only 0.1 g of plant material and without creating artefacts during isolation.</li><li>Volatiles stored in twigs are separated from needle volatiles and thus add new information to chemophenetic studies.</li><li>Volatiles of 100 individuals of <em>P. omorika</em> collected in natural populations from seven localities were extracted for the first time using by HS. The composition was analysed statistically to assess variability and population differentiation.</li><li>The dominant compounds were <em>α</em>-pinene (29.5 %), <em>β</em>-pinene (25.7%), and myrcene (13.0%), representing 68.2% of the HS volatiles on average.</li></ul><p class="normal"><strong><br /></strong></p><p class="normal"><strong>Abstract:</strong> The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of <em>Picea omorika</em> from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were <em>α</em>-pinene (29.5 %), <em>β</em>-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene,<em> α-</em>phellandrene, <em>δ</em>-3-carene, <em>p</em>-cymene, <em>β-</em>phellandrene, terpinolene, (<em>E</em>)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (<em>α</em>-pinene, <em>β</em>-pinene, myrcene, <em>δ</em>-3-carene, <em>β</em>-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.</p><p class="normal"><a href="https://doi.org/10.2298/ABS200511038N">https://doi.org/10.2298/ABS200511038N</a></p><div><p class="normal"><strong>Received:</strong> May 11, 2020; <strong>Revised:</strong> August 19, 2020; <strong>Accepted:</strong> August 24, 2020; <strong>Published online: </strong>August 27, 2020</p><p class="normal"><strong>How to cite this article:</strong> Nikolić B, Ljujić J, Bojović S, Mitić Z, Rajčević N, Tešević V, Marin PD. Headspace volatiles isolated from twigs of <em>Picea omorika</em> from Serbia. Arch Biol Sci. 2020;72(3):445-52.</p></div></div>}, number={3}, journal={Archives of Biological Sciences}, author={Nikolić, Biljana and Ljujić, Jovana and Bojović, Srđan and Mitić, Zorica and Rajčević, Nemanja and Tešević, Vele and Marin, Petar D.}, year={2020}, month={Oct.}, pages={445–452} }