@article{Stojanović_Milošević_Vitorović_Savić Zdravković_Stanković_Stanković_Vasiljević_2021, title={Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles}, volume={73}, url={https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6624}, DOI={10.2298/ABS210515025S}, abstractNote={<p><strong>Paper description:</strong></p> <ul> <li><em>Chironomus riparius</em> is a model organism in ecotoxicology. Histological analysis of its internal morphology could help identify biomarkers of the effects of sublethal concentrations of pollutants.</li> <li>4<sup>th</sup> instar larvae of <em> riparius </em>were exposed to sublethal concentrations of TiO<sub>2</sub>, CeO<sub>2</sub> and Fe<sub>3</sub>O<sub>4</sub> nanoparticle and histological analysis was performed.</li> <li>Nano-Fe<sub>3</sub>O<sub>4</sub> and nano-Fe<sub>3</sub>O<sub>4</sub>/nano-CeO<sub>2</sub> exposure caused vacuolization of epithelial cells of midgut region I and Malpighian tubules, respectively; nano-TiO<sub>2</sub> exposure altered fat body and midgut region II tissue architecture; nano-Fe<sub>3</sub>O<sub>4 </sub>shortened the intestinal brush border.</li> <li>The identified histopathological biomarkers of <em> riparius</em> larvae improve existing methodology in water quality assessment.</li> </ul> <p><strong>Abstract: </strong>As the production of metal-based nanomaterials increases, it is inevitable that nano-scale products and byproducts will enter the aquatic environment. In terms of global production, the most abundant nano-oxides are TiO<sub>2</sub>, CeO<sub>2</sub> and Fe<sub>3</sub>O<sub>4</sub> nanoparticles. <em>Chironomus</em> <em>riparius</em> is commonly used for ecotoxicological assessment and defining its histopathological biomarkers that showcase the toxic effect of tested nanoparticles should lead to a better understanding of the consequences of nanomaterial accumulation in aquatic ecosystems. In this study, a histological description of the digestive and excretory systems as well as the fat body structure of <em>C. riparius</em> larvae is provided. In addition, potential histological biomarkers of nano-oxide toxicity were determined based on the obtained histopathological alterations in organs. Vacuolization was observed in epithelial cells of midgut region I that were treated with nano-Fe<sub>3</sub>O<sub>4</sub> as well as in Malpighian tubules treated with nano-Fe<sub>3</sub>O<sub>4</sub> and nano-CeO<sub>2</sub>. Larvae exposed to nano-TiO<sub>2</sub> showed alterations in the fat body and midgut region II tissue architecture. Additionally, shortening of the intestinal brush border was determined in groups exposed to nano-Fe<sub>3</sub>O<sub>4</sub>. These results reveal the high sensitivity of these organs, which can be used as biomarkers in histopathological assessment and therefore lead to further improvement of existing methodology in ecotoxicological studies.</p> <p> </p>}, number={3}, journal={Archives of Biological Sciences}, author={Stojanović, Jelena S. and Milošević, Đurađ D. and Vitorović, Jelena S. and Savić Zdravković, Dimitrija N. and Stanković, Nikola R. and Stanković, Jelena B. and Vasiljević, Perica J.}, year={2021}, month={Oct.}, pages={319–329} }