TY - JOUR AU - Stanojević, Olja AU - Milijašević-Marčić, Svetlana AU - Potočnik, Ivana AU - Stepanović, Miloš AU - Dimkić, Ivica AU - Stanković, Slaviša AU - Berić, Tanja PY - 2016/11/25 Y2 - 2024/03/28 TI - ISOLATION AND IDENTIFICATION OF BACILLUS SPP. FROM COMPOST MATERIAL, COMPOST AND MUSHROOM CASING SOIL ACTIVE AGAINST TRICHODERMA SPP. JF - Archives of Biological Sciences JA - Arch Biol Sci VL - 68 IS - 4 SE - Articles DO - UR - https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1258 SP - 845-852 AB - <div><p>The isolation of bacteria was carried out from samples of straw and chicken manure, compost at various stages of the composting process and casing soil used for growing button mushrooms. A preliminary screening of 108 bacterial isolates for antagonistic activity against <em>Trichoderma aggressivum</em> f. <em>europaeum</em> showed that 23 tested isolates inhibited mycelial growth of the pathogenic fungus. Further screening with four indicator isolates of fungi revealed that all 23 bacterial isolates inhibited the growth of <em>T. aggressivum </em>f.<em> europaeum</em>, <em>T. harzianum</em> and <em>T. koningii</em>, while only 13 isolates inhibited the growth of <em>T. atroviride</em>. <em>T. aggressivum</em> f. <em>europaeum</em> proved to be the most sensitive, with many bacterial isolates generating a high percentage of growth inhibition. Only two bacterial isolates (B-129 and B-268) were successful in inhibiting the growth of all 4 tested pathogens. All 23 bacterial isolates were characterized as Gram-positive and catalase-positive and were subjected to molecular identification based on the partial sequence, the hypervariant region of the 16S rDNA. It was shown that the obtained bacterial strains belong to <em>Bacillus subtilis</em>, <em>B. amyloliquefaciens</em>, <em>B. licheniformis</em> and <em>B. pumilus</em> species.</p></div><p align="left"><strong>DOI:</strong> <a href="http://doiserbia.nb.rs/Article.aspx?id=0354-46641600073S">10.2298/ABS151104073S</a></p><div><p align="left"><strong>Key words:</strong> <em>Agaricus bisporus</em>; <em>Bacillus</em>; biocontrol; green mold; hypervariant region</p><p align="left"><strong>Received</strong>: November 4, 2015; <strong>Revised</strong>: December 25, 2015; <strong>Accepted</strong>: January 21, 2016; <strong>Published online:</strong> August 9, 2016</p><p align="left"><strong>How to cite this article:</strong> Stanojević O, Milijašević-Marčić S, Potočnik I, Stepanović M, Dimkić I, Stanković S, Berić T. Isolation and identification of Bacillus spp. from compost material, compost and mushroom casing soil active against Trichoderma spp.. Arch Biol Sci. 2016;68(4):845-52.</p></div> ER -