TY - JOUR AU - Jović, Saša AU - Zeljić, Katarina AU - Ivković, Nemanja AU - Štefik, Debora AU - Kozomara, Ružica AU - Stošić, Srboljub AU - Šupić, Gordana PY - 2021/12/15 Y2 - 2024/03/29 TI - Cyclin D1 and p21 gene variants and oral squamous cell carcinoma risk and prognosis JF - Archives of Biological Sciences JA - Arch Biol Sci VL - 73 IS - 4 SE - Articles DO - 10.2298/ABS210813037J UR - https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6911 SP - 437-445 AB - <p><strong>Paper description: </strong></p><ul><li>p21 and cyclin D1, encoded by the <em>CDKN1A </em>and the <em>CCND1 </em>genes, respectively, are important regulators of cell cycle progression and could be associated with oral cancer.</li><li>Gene variants within the <em>CDKN1A</em> (rs1801270, rs1059234) and <em>CCND1</em> (rs9344) were genotyped by real-time PCR in a group of 104 oral cancer patients and 107 healthy controls.</li><li><em>CCND1</em> polymorphism rs9344 was significantly associated with increased oral cancer risk but not with its prognosis.</li><li>Variant rs9344 in the <em>CCND1</em> gene could be considered as a potential marker for oral cancer susceptibility and might be potentially useful for future cancer risk assessment.</li></ul><p><strong>Abstract: </strong>Cyclin-dependent kinase inhibitor p21 (encoded by the <em>CDKN1A</em> gene) and cyclin D1 (encoded by the <em>CCND1</em> gene) are important regulators of cell cycle progression and could have important effects in the complex process of neoplastic transformation. The current study aimed to investigate variants in <em>CDKN1A</em> (rs1801270, rs1059234) and <em>CCND1 </em>(rs9344) genes as potential risk and prognostic factors in oral squamous cell carcinoma (OSCC) patients. The study included 104 OSCC patients and 107 healthy individuals without a history of cancer. Genotypes were assessed by real-time PCR and TaqMan SNP genotyping. Significant differences in genotype distribution between OSCC cases and the control group were observed for the <em>CCND1 </em>rs9344 variant (p=0.017). According to the odds ratio (OR), adjusted for age and sex, the rs9344 heterozygous GA and homozygous mutated AA genotypes were associated with an increased OSCC susceptibility (OR=2.295, p=0.007; OR=2.029, p=0.037, respectively). Variants rs1801270 and rs105923 in <em>CDKN1A </em>were not associated with OSCC risk. There were no differences in overall survival among OSCC patients stratified by genotypes of the analyzed variants in <em>CDKN1A </em>and <em>CCND1</em>. Variant rs9344 in the <em>CCND1</em> gene might be considered as a potential molecular risk factor for OSCC susceptibility but not for disease prognosis.</p> ER -