TY - JOUR AU - Wu, Xiaofeng AU - Zhou, Zihui PY - 2017/10/18 Y2 - 2024/03/28 TI - Reduced cell proliferation and promotion of apoptosis by Leu5AMD in osteosarcoma cells JF - Archives of Biological Sciences JA - Arch Biol Sci VL - 69 IS - 4 SE - Articles DO - UR - https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/817 SP - 577-583 AB - <div><p>Leu<sup>5</sup>AMD is an actinomycin D (AMD) analog obtained by replacing both N-methylvalines present in AMD with N-methylleucines. The potential of Leu<sup>5</sup>AMD for the treatment of osteosarcoma cells and its mechanism of action are not clearly understood. In this study, we examined the potential use of Leu<sup>5</sup>AMD in halting the proliferation of the human osteosarcoma cell line Saos-2. Saos-2 cells were treated with different concentrations of Leu<sup>5</sup>AMD, and proliferation, cell viability, apoptosis and the cell cycle were examined. Among the various concentrations used, 1.2 μM and 6 μM Leu<sup>5</sup>AMD exhibited a significant effect on Saos-2 cells compared with untreated cells. The proliferation assay and Hoechst staining indicated that Leu<sup>5</sup>AMD treatment of Saos-2 human osteosarcoma cells resulted in a significant reduction in cell proliferation and enhanced apoptosis. Western blot analysis revealed that Leu<sup>5</sup>AMD-treated cells had significantly reduced cyclin expression compared with the control. Leu<sup>5</sup>AMD is responsible for inducing cell cycle arrest and apoptosis. These results support the development of a new drug for the treatment of osteosarcoma.</p><p><a href="https://doi.org/10.2298/ABS160712001W">https://doi.org/10.2298/ABS160712001W</a></p><p><strong>Received:</strong> July 12, 2016; <strong>Revised:</strong> December 2, 2016; <strong>Accepted:</strong> December 2, 2016; <strong>Published online:</strong> August 9, 2017</p><p><strong>How to cite this article: </strong>Wu X, Zhou Z.<strong> </strong>Reduced cell proliferation and promotion of apoptosis by Leu5AMD in osteosarcoma cells. Arch Biol Sci. 2017;69(4):577-83.</p></div> ER -