TY - JOUR AU - Han, Haote AU - Liu, Na AU - Zhang, Lin AU - Gong, Minghua AU - Cao, Ming AU - Li, Baoguo AU - Kaisa, Sulaiman AU - Yu, Xiuying AU - Tian, Jingkui PY - 2017/08/11 Y2 - 2024/03/29 TI - Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. JF - Archives of Biological Sciences JA - Arch Biol Sci VL - 69 IS - 3 SE - Articles DO - UR - https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/960 SP - 481-489 AB - <p>We investigated the antitumor activity of daucusol (DS) derived from<em> Daucus carota </em>L. in PC-3, A549 and HeLa cell lines by the MTT assay. Optical microscopy revealed that exposure of PC-3 cells to DS resulted in cytoplasmic vacuolation. Flow cytometry analysis of the phase of the cell cycle did not reveal a sub-G1 peak, and no caspase-dependent activation was observed after DS treatment. The levels of endoplasmic reticulum (ER) stress biomarkers, LC3B-II and ubiquitinated proteins were increased. It was also observed that oxidative stress played an important role in the activation of the cytoplasmic vacuolation-mediated cell-death pathway. <em>I</em><em>n vivo</em>, DS inhibited tumor growth in nude mice by 39.13% compared to the vehicle. Protein expression in the tumor tissue was consistent with their expression <em>in vitro</em>. Our findings indicate that DS induced cytoplasmic vacuolation-mediated death in PC-3 cells by triggering oxidative stress and suggest that targeting this pathway could serve as a novel therapeutic approach for prostate cancer.</p><p align="left"><a href="https://doi.org/10.2298/ABS160902123H" target="_blank">https://doi.org/10.2298/ABS160902123H</a></p><p><strong>Received:</strong> September 2, 2016; <strong>Revised:</strong> October 1, 2016; <strong>Accepted:</strong> October 28, 2016; <strong>Published online:</strong> November 9, 2016</p><p align="left"><strong>How to cite: </strong>Han H, Liu N, Zhang L, Gong M, Cao M, Li B, Kaisa S, Yu X, Tian J. Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. Arch Biol Sci. 2017;69(3):481-9.</p> ER -