A comparative study of the antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro- propagated plants of endemic Digitalis trojana Ivanina

Nurşen Çördük, Sefer Demirbas, Nurcihan Hacıoglu Dogru

Abstract


The antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro-propagated plants of Digitalis trojana Ivanina (Helen of Troy foxglove), a perennial endemic plant species of Turkey, were compared. The field work was carried out in May and July 2014, and plant samples of D. trojana were collected from Kazdağı (Turkey). Propagation of D. trojana was achieved by culturing leaf explants on MS medium supplemented with 13.3 µM 6-benzylaminopurine (BA) and 0.53 µM α-naphthaleneacetic acid (NAA). The antimicrobial activity, plant lipid peroxidation levels and antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR)) activities were analyzed in 12- and 17-week-old in vitro-grown Digitalis plants collected in May and July from two different localities at 430 and 1173 m above sea level. Although the in vitro-propagated plants had very low antagonistic activities compared to field-grown plants, they exhibited remarkably similar antibacterial activities against Escherichia coli ATCC 11230, Pseudomonas aeruginosa ATCC 27853 and Bacillus subtilis ATCC 6633. There were no important differences between plants collected from the two localities (430 and 1173 m a.s.l.). Biochemical analysis showed that the antioxidant enzyme (SOD, APX, GR) activities of field-grown plants were higher than in vitro-grown plants. Also, the difference in altitude at which the plants were grown was apparently linked to decreases in antioxidant enzyme activities, except for POX in field-grown plants collected in July.

https://doi.org/10.2298/ABS161216004C

Received: December 16; 2016; Revised: January 6, 2017; Accepted: February 2, 2017; Published online: March 10, 2017

How to cite this article: Corduk N, Demirbas S, Hacioglu Dogru N. A comparative study of the antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro- propagated plants of endemic Digitalis trojana Ivanina. Arch Biol Sci. 2017;69(4):603-10.


Keywords


Digitalis trojana; in vitro; field-grown; antimicrobial activity; antioxidant enzyme activities

Full Text:

PDF

References


Uysal İ, Öztürk M. Digitalis trojana Ivan. Endemik türünün morfolojisi, anatomisi ve ekolojisi. Anadolu University Journal of Faculty of Arts and Sciences. 1991;3:53-61. Turkish.

Ekim T, Koyuncu M, Vural M, Duman H, Aytaç Z, Adıgüzel N. Red data book of Turkish plants (Pteridophyta and Spermatophyta). Ankara: Foundation for Turkish Nature Conservation and Van Centinential University Press; 2000.

Winnicka W, Bielawski K, Bielawska A. Cardiac glycosides in cancer research and cancer therapy. Acta Pol Pharm. 2006;63:109-15.

Padua R, Waibel R, Kuate SP, Schebitz PK, Hahn S, Gmeiner P, Kreis W. A Simple Chemical Method for Synthesizing Malonyl Hemiesters of 21 hydroxypregnanes, Potential Intermediates in Cardenolide Biosynthesis. Steroids. 2008;73:458-65.

Elbaz H, Stueckle T, Tse W, Rojanasakul Y, Dinu C. Digitoxin and its analogs as novel cancer therapeutics. Exp Hematol Oncol. 2012;1(1):4.

Vats S, Tiwari R, Alam A, Behera KK, Pareek R. Evaluation of phytochemicals, antioxidant and antimicrobial activity of in vitro culture of Vigna unguiculata L. Walp. Researcher. 2012;4:70-4.

Roia FC, Smith R. The Antibacterial Screening of Some Common Ornamental Plants. Econ Bot. 1977;31:28-37.

Benli M, Yigit N, Geven F, Guney K, Bingol U. Antimicrobial activity of endemic Digitalis lamarckii Ivan from Turkey. Indian J Exp Biol. 2009;47:218-21.

Suzuki N, Mittler R. Reactive oxygen species and temperature stresses: A delicate balance between signaling and destruction. Physiol Plant. 2006;126:45-51.

Foyer CH, Noctor G. Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ. 2005;28:1056-71.

Wildi B, Lütz C. Antioxidant composition of selected high alpine plant species from different altitudes. Plant Cell Envıron. 1996;19:138-46.

Streb P, Feierabend J, Bligny R. Resistance to photoinhibition of photosystem II and catalase and antioxidative protection in high mountain plants. Plant Cell Environ. 1997;20:1030-40.

Çördük N, Akı C. Direct Shoot Organogenesis of Digitalis trojana Ivan. an Endemic Medicinal Herb of Turkey. Afr J Biotechnol. 2010;9:1587-91.

Murashige T, Skoog F. A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Culture. Physiol Plant. 1962;15:473-97.

Khan NH, Nur-E-Kamal MSA, Rahman M. Antimicrobial Activity of Euphorbia thymifolia Linn. Indian J Med Res. 1988;87:395-7.

Collins CH, Lyne PM Grange JM. Microbiological Methods. 6th ed. London: Butterworths and Co. Ltd.; 1989. 410 p.

Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 1998;29:129-32

Madhava Rao KV, Sresty TVS. Antioxidative Parameters in The Seedlings of Pigeonpea (Cajanus cajan L. Millspaugh) in Response to Zn and Ni Stresses. Plant Sci. 2000;157:113-28.

Bradford M. A Rapid and Sensitive Method for the Quantition of Microgram Quantities of Protein Utilizing the Principle of Protein-dye Binding. Anal Biochem. 1976;72:248-54.

Beauchamp C, Fridovich I. Superoxide Dismutase: Improved Assay and Applicable to Acrylamid Gels, Anal Biochem. 1971;44:276-87.

Giannopolitis N, Ries SK. Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol. 1977;59:309-14.

Aebi H. Katalase. In: Bergmeyer N, editor. Methoden Der Enzymatischen Analyse. Vol 1, Berlin: Akademie Verlag; 1970. p. 636-47.

Foyer CH, Halliwell B. Presence of Glutathione and Glutathione Reductase in Chloroplasts: A Proposed Role in Ascorbic Acid Metabolism. Planta. 1976;133:21-5.

Nakano Y, Asada K. Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts. Plant Cell Physiol. 1981;22:867-80.

Kanner J, Kinsella JE. Lipid Deterioration Initiated by Phagocytic-Cells in Muscle Foods-Beta-Carotene Destruction by a Myeloperoxidase Hydrogen-Peroxide Halide System. J Agric Food Chem. 1983;31:370-6.

Parsaeimehr A, Sargsyan E, Javidnia K. A Comparative Study of the Antibacterial, Antifungal and Antioxidant Activity and Total Content of Phenolic Compounds of Cell Cultures and Wild Plants of Three Endemic Species of Ephedr. Molecules. 2010;15:1668-78.

Kumari A, Baskaran P, Staden JV. In vitro propagation and antibacterial activity in Cotyledon orbiculata: a valuable medicinal plant. Plant Cell Tiss Org. 2016;124:97-104.

Jonathan SG, Fasidi IO. Antimicrobial activities of two Nigerian edible macrofungi, Lycoperdon pusillum and L. giganteum. Afr J Biomed Res. 2003;6:85-90.

García-Pérez E, Gutiérre-Uribe JA, García-Lara S. Luteolin content and antioxidant activity in micropropagated plants of Poliomintha glabrescens (Gray). Plant Cell Tiss Org. 2012;108:521-7.

Baskaran P, Singh S, Van Staden J. In vitro propagation, proscillaridin A production and antibacterial activity in Drimia robusta. Plant Cell Tiss Org. 2013;114:259-67.

Baskaran P, Chukwujekwu JC, Amoo SO, Van Staden J. Anticholinesterase and mutagenic evaluation of in vitro-regenerated Agapanthus praecox grown ex vitro. In Vitro Cell Dev Biol Plant. 2014;50:271-5.

Thakar J, Bhargava S. Seasonal variation in antioxidant enzymes and the sprouting response of Gmelina arborea Roxb. nodal sectors cultured in vitro. Plant Cell Tiss Org. 1999;59:181-7.

Shohael AM, Ali MB, Yu KW, Hahn EJ, Paek KY. Effect of temperature on secondary metabolites production and antioxidant enzyme activities in Eleutherococcus senticosus somatic embryos. Plant Cell Tiss Org. 2006;85: 219-28.

Filipović BK, Simonović AD, Trifunović MM, Dmitrović SS, Savić JM, Jevremović SB, Subotić AR. Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 1: The role of antioxidant enzymes. Plant Cell Tiss Org. 2015;121:703-19.

Miszalski Z, Libik M, Surówka E, Niewiadomska E. Cu/Zn superoxide dismutase and catalase activities in Pinus mugo needles growing at elevated stands in the mountains, and their photochemical efficiency of PSII. J Plant Physiol. 2005;162:895-902.

Ren HX, Wang ZL, Chen X, Zhu YL. Antioxidative responses to different altitudes in Plantago major. Environ Exp Bot. 1999;42:51-59.

Wang Y, He W, Huang H, An L, Wang D, Zhang F. Antioxidative responses to different altitudes in leaves of alpine plant Polygonum viviparum in summer. Acta Physiol Plant. 2009;31:839-48.

Öncel I, Yurdakulol E, Keleş Y, Kurt L, Yıldız A. Role of antioxidant defense system and biochemical adaptation on stress tolerance of high mountain and steppe plants. Acta Oecol. 2004;26:211-8.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 ARCHIVES OF BIOLOGICAL SCIENCES

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.