Carnosol promotes endothelial differentiation under H2O2-induced oxidative stress

Shulin Ou, Jianfeng Lv, Liming Peng, Jin Zhao, Luxiang Chi

Abstract


Oxidative stress causes deregulation of endothelial cell differentiation. Carnosol is a potent antioxidant and antiinflammatory compound. In the present study, we examined whether the antioxidant effect of carnosol might protect bone marrow stem cells against H2O2-induced oxidative stress and promote endothelial differentiation. We examined cell viability by the MTT assay; oxidative stress and apoptosis were analyzed through changes in ROS levels, apoptotic ratio and caspase-3 activity; changes in protein expression of OCT-4, Flk-1, CD31 and Nrf-2 were assessed by Western blot analysis. H2O2 treatment increased oxidative stress and reduced cell viability, while the stem cell marker OCT-4 and endothelial markers Flk-1, CD31 were significantly downregulated as a result of the treatment with H2O2. Treatment with carnosol improved the antioxidant status, increased OCT-4 expression and promoted endothelial differentiation. This study provides evidence that carnosol could increase the antioxidant defense mechanism and promote endothelial differentiation.

https://doi.org/10.2298/ABS160327101O

Received: March 27, 2016; Revised: June 20, 2016; Accepted: July 10, 2016; Published online: October 21, 2016

How to cite this article: Ou S, Lv J, Peng L, Zhao J, Chi L. Carnosol promotes endothelial differentiation under H2O2-induced oxidative stress. Arch Biol Sci. 2017;69(2):299-304.


Keywords


carnosol; differentiation; endothelial cells; Nrf-2; oxidative stress; ROS

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