Protective effect of the Rubus chingii Hu. fruit extract on ultraviolet B-induced photoaging via suppression of mitogen-activated protein kinases (MAPKs) in vitro

Jie-Mei She, Hui-Hui Yuan, Jin Zhang, Zhao-Nian Si, Min-Bo Lan

Abstract


Paper description:

  • Rubus chingii Hu possesses antioxidant, anti-inflammatory and anti-aging activities.
  • The ethanol extract of the fruit (E-EA) demonstrated significant anti-photoaging properties in UVB-induced human fibroblast (HFF-1) cells, observed as a decrease in levels of reactive oxygen species (ROS), inhibition of matrix metallopeptidase 1 (MMP-1) production, and promotion of type-I procollagen production via suppression of the mitogen-activated protein kinase (MAPK) signaling pathway.
  • E-EA is suggested as a potential anti-photoaging agent for the protection of skin from UVB damage.

Abstract: Rubus chingii Hu. is an important traditional Chinese medicine with a beneficial effect on the kidney. Traditional Chinese medicine theory believes that supporting the kidney has a key role in anti-aging. To the best of our knowledge, the anti-photoaging activity of Rubus chingii Hu. has not been investigated. We investigated the anti-photoaging effects and the underlying mechanism of the ethyl acetate fraction of Rubus chingii Hu. fruit ethanol extract (E-EA) in UVB-induced human fibroblasts (HFF-1). Our results show that E-EA (10-40 μg/mL) displayed preventive and therapeutic effects on UVB-induced cytotoxicity in HFF-1 cells. E-EA also presented significant anti-photoaging properties in UVB-induced HFF-1 cells by decreasing the levels of reactive oxygen species (ROS), inhibiting matrix metallopeptidase 1 (MMP-1) production and promoting type-I procollagen production via suppression of the mitogen-activated protein kinase (MAPK) signaling pathway. These results suggested that E-EA might be a promising anti-photoaging agent to protect the skin from UVB damage, with a potential application in cosmetics.

https://doi.org/10.2298/ABS190321038S

Received: March 21, 2019; Revised: May 3, 2019; Accepted: June 3, 2019; Published online: July 7, 2019

How to cite this article: She J, Yuan H, Zhang J, Si Z, Lan M. Protective effect of the Rubus chingii Hu. fruit extract on ultraviolet B-induced photoaging via suppression of mitogen-activated protein kinases (MAPKs) in vitro. Arch Biol Sci. 2019;71(3):541-50.


Keywords


Rubus chingii Hu.; antioxidant activity; anti-photoaging; reactive oxygen species (ROS); mitogen-activated protein kinase (MAPK) signaling pathway

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References


Gragnani A, Cornick SM, Chominski V, Ribeiro de Noronha SM, Alves Corrêa de Noronha SA, Ferreira LM. Review of major theories of skin aging. Adv Aging Res. 2014;03(04):265-84.

Cavinato M, Jansen-Durr P. Molecular mechanisms of UVB-induced senescence of dermal fibroblasts and its relevance for photoaging of the human skin. Exp Gerontol. 2017;94:78-82.

Lee CH, Wu SB, Hong CH, Yu HS, Wei YH. Molecular mechanisms of UV-induced apoptosis and its effects on skin residential cells: the implication in UV-based phototherapy. Int J Mol Sci. 2013;14(3):6414-35.

Santhanam RK, Fakurazi S, Ahmad S, Abas F, Ismail IS, Rukayadi Y, Akhtar MT, Shaari K. Inhibition of UVB-induced pro-inflammatory cytokines and MMP expression by Zanthoxylum rhetsa bark extract and its active constituent hesperidin. Phytother Res. 2018;32(8):1608-16.

Chiocchio I, Mandrone M, Sanna C, Maxia A, Tacchini M, Poli F. Screening of a hundred plant extracts as tyrosinase and elastase inhibitors, two enzymatic targets of cosmetic interest. Ind Crop Prod. 2018;122:498-505.

Lu J, Guo JH, Tu XL, Zhang C, Zhao M, Zhang QW, Gao FH. Tiron inhibits UVB-induced AP-1 binding sites transcriptional activation on MMP-1 and MMP-3 promoters by MAPK signaling pathway in human dermal fibroblasts. PLoS One. 2016;11(8):e0159998.

Gilchrest BA. Photoaging. J Invest Dermatol. 2013;133(E1):E2-6.

Zeng HJ, Liu Z, Wang YP, Yang D, Yang R, Qu LB. Studies on the anti-aging activity of a glycoprotein isolated from Fupenzi (Rubus chingii Hu.) and its regulation on klotho gene expression in mice kidney. Int J Biol Macromol. 2018;119:470-6.

Rowland J, Akbarov A, Eales J, Xu X, Dormer JP, Guo H, Denniff M, Jiang X, Ranjzad P, Nazgiewicz A, Prestes PR, Antczak A, Szulinska M, Wise IA, Zukowska-Szczechowska E, Bogdanski P, Woolf AS, Samani NJ, Charchar FJ, Tomaszewski M. Uncovering genetic mechanisms of kidney aging through transcriptomics, genomics, and epigenomics. Kidney Int. 2019;95(3):624-35.

Li G, Chen Y, Hu H, Liu L, Hu X, Wang J, Shi W, Yin D. Association between age-related decline of kidney function and plasma malondialdehyde. Rejuv. Res. 2012;15(3):257-64.

Schmidlin CJ, Dodson MB, Madhavan L, Zhang DD. Redox regulation by NRF2 in aging and disease. Free Radic Biol Med. 2019;134:702-4.

Yau M-H, Che C-T, Liang S-M, Kong Y-C, Fong W-P. An aqueous extract of Rubus chingii fruits protects primary rat hepatocytes against tert-butyl hydroperoxide induced oxidative stress. Life Sci. 2002;72(3):329-38.

Zeng H, Yang R, Lei L, Wang Y. Total flavonoid content, the antioxidant capacity, fingerprinting and quantitative analysis of fupenzi (Rubus chingii Hu.). Chin Med. 2015;06(04):204-13.

Zhang TT, Liu YJ, Yang L, Jiang JG, Zhao JW, Zhu W. Extraction of antioxidant and antiproliferative ingredients from fruits of Rubus chingii Hu by active tracking guidance. Medchemcomm. 2017;8(8):1673-80.

Zhang TT, Wang M, Yang L, Jiang J-G, Zhao J-W, Zhu W. Flavonoid glycosides from Rubus chingii Hu fruits display anti-inflammatory activity through suppressing MAPKs activation in macrophages. J Funct Foods. 2015;18:235-43.

Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci Technol. 1995;28(1):25-30.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9-10):1231-7.

Stanic-Vucinic D, Prodic I, Apostolovic D, Nikolic M, Velickovic TC. Structure and antioxidant activity of beta-lactoglobulin-glycoconjugates obtained by high-intensity-ultrasound-induced Maillard reaction in aqueous model systems under neutral conditions. Food Chem. 2013;138(1):590-9.

Meyers KJ, Watkins CB, Pritts MP, Liu RH. Antioxidant and antiproliferative activities of strawberries. J Agric Food Chem. 2003;51(23):6887-92.

Xuan SH, Park YM, Park SH, Jeong HJ, Park SN. Suppression of Ultraviolet B-mediated matrix metalloproteinase generation by Sorbus commixta Twig extract in human dermal fibroblasts. Photochem Photobiol. 2018;94(2):370-7.

Vauzour D, Rodriguez-Mateos A, Corona G, Oruna-Concha MJ, Spencer JP. Polyphenols and human health: prevention of disease and mechanisms of action. Nutrients. 2010;2(11):1106-31.

Prochazkova D, Bousova I, Wilhelmova N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 2011;82(4):513-23.

Pandey S, Tiwari S, Kumar A, Niranjan A, Chand J, Lehri A, Chauhan PS. Antioxidant and anti-aging potential of Juniper berry (Juniperus communis L.) essential oil in Caenorhabditis elegans model system. Ind Crop Prod. 2018;120:113-22.

Boran R. Investigations of anti-aging potential of Hypericum origanifolium Willd. for skincare formulations. Ind Crop Prod. 2018;118:290-5.

Saewan N, Jimtaisong A. Photoprotection of natural flavonoids. J App Pharm Sci. 2013;3(09):129-41.

Zakaria NNA, Okello EJ, Howes MJ, Birch-Machin MA, Bowman A. In vitro protective effects of an aqueous extract of Clitoria ternatea L. flower against hydrogen peroxide-induced cytotoxicity and UV-induced mtDNA damage in human keratinocytes. Phytother Res. 2018;32(6):1064-72.

Liu S, You L, Zhao Y, Chang X. Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and Type I Procollagen production in mice. J Agric Food Chem. 2018;66(32):8537-46.

Kim H, Cho H, Seo Y-K, Kim S, Yoon MY, Kang H, Park CS, Park JK. Inhibitory effects of sea buckthorn (Hippophae rhamnoides L.) seed on UVB-induced Photoaging in human dermal fibroblasts. Biotechnol Bioprocess Eng. 2012;17(3):465-74.

Hwang E, Lin P, Ngo HTT, Yi TH. Clove attenuates UVB-induced photodamage and repairs skin barrier function in hairless mice. Food Funct. 2018;9(9):4936-47.

Li L, Hwang E, Ngo HTT, Lin P, Gao W, Liu Y, Yi TH. Antiphotoaging effect of Prunus yeonesis Blossom extract via inhibition of MAPK/AP-1 and regulation of the TGF-βI/Smad and Nrf2/ARE signaling pathways. Photochem Photobiol. 2018;94(4):725-32.

Duque L, Bravo K, Osorio E. A holistic anti-aging approach applied in selected cultivated medicinal plants: A view of photoprotection of the skin by different mechanisms. Ind Crop Prod. 2017;97:431-9.

Huang CY, Lin YT, Kuo HC, Chiou WF, Lee MH. Compounds isolated from Eriobotrya deflexa leaves protect against ultraviolet radiation B-induced photoaging in human fibroblasts. J Photochem Photobiol B: Biol. 2017;175:244-53.


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