Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L.

Haote Han, Na Liu, Lin Zhang, Minghua Gong, Ming Cao, Baoguo Li, Sulaiman Kaisa, Xiuying Yu, Jingkui Tian


We investigated the antitumor activity of daucusol (DS) derived from Daucus carota L. in PC-3, A549 and HeLa cell lines by the MTT assay. Optical microscopy revealed that exposure of PC-3 cells to DS resulted in cytoplasmic vacuolation. Flow cytometry analysis of the phase of the cell cycle did not reveal a sub-G1 peak, and no caspase-dependent activation was observed after DS treatment. The levels of endoplasmic reticulum (ER) stress biomarkers, LC3B-II and ubiquitinated proteins were increased. It was also observed that oxidative stress played an important role in the activation of the cytoplasmic vacuolation-mediated cell-death pathway. In vivo, DS inhibited tumor growth in nude mice by 39.13% compared to the vehicle. Protein expression in the tumor tissue was consistent with their expression in vitro. Our findings indicate that DS induced cytoplasmic vacuolation-mediated death in PC-3 cells by triggering oxidative stress and suggest that targeting this pathway could serve as a novel therapeutic approach for prostate cancer.

Received: September 2, 2016; Revised: October 1, 2016; Accepted: October 28, 2016; Published online: November 9, 2016

How to cite: Han H, Liu N, Zhang L, Gong M, Cao M, Li B, Kaisa S, Yu X, Tian J. Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. Arch Biol Sci. 2017;69(3):481-9.


daucusol; cytoplasmic vacuolation; oxidative stress; endoplasmic reticulum stress; prostate cancer

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