Mehmet Karataş, Muhammad Aasim, Muraz Dazkirli


Water hyssop (Bacopa monnieri (L.) Pennell) is a medicinal plants. Its upper and lower halves of leaf explants were incubated in Murashige and Skoog (MS) medium supplemented with 0.25, 0.50 and 1.0 mg/L benzylaminopurine (BA) for 8 weeks; the explants were exposed to white (W) and red and blue (R and B, respectively) light-emitting diodes (LEDs), at 4:1, 3:1, 2:1 and 1:1 R and B light ratios, respectively. Shoot regeneration (100%) was achieved from all explants at all applied concentrations of BA and LED types. All explants showed different BA concentration requirements for regeneration of the maximum number of shoots. Longer shoots were obtained on medium with 0.25 mg/L BA. The W LED lighting system was found to be more effective for regenerating the maximum number of shoots (26.11) per explant (on the upper half of the leaf). Conversely, longer and shorter shoots were generated under 1:1 R:B and W LEDs, respectively. The number of shoots per explant ranged from 9.67-24.0 (full leaf), 6.33-25.92 (lower half of the leaf) and 7.33-27.33 (upper half of the leaf), respectively, in response to BA and LED light. Shoot length ranged from 0.94-1.90 cm (full lamina), 0.70-2.11 cm (lower half of the leaf) and 0.93-1.83 cm (upper half of the lamina) in response to BA and LED lifght. Regenerated shoots were successfully rooted using indole-3-butyric acid (IBA) and acclimatized in the aquarium provided with tap water.


Key words: adventitious; light-emitting diodes (LEDs); leaf explants; shoot regeneration; water hyssop


Received: August 3, 2015; Revised: November 13, 2015; Accepted: November 16, 2015; Published online: April 22, 2016

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Tiwari KN, Singh J. Effective organogenesis from different explants of Bacopa monnieri L. (Wettst.) − An important medicinal plant. BFIJ. 2010;2:18-22.

Soundararajan T, Karrunakaran CM. Micropropagation of Bacopa monnieri through protoplast. Asian J Biotechnol. 2011;3:135-52.

Joshi AG, Pathak AR, Sharma AM, Singh S. High frequency of shoot regeneration on leaf explants of Bacopa monnieri. Environ Exp Biol. 2010;8:81-4.

Ali G, Srivastava PV, Iqbal M. Morphogenic and biochemical responses of Bacopa monnieri cultures to zinc toxicity. Plant Sci. 1999;143:187-93.

Mukherjee DG, Dey CD. Clinical trial on Brahmi. I. J Exper Med Sci. 1996;10:5-11.

Vijayakumar M, Vijayakumar R, Stephen R. In vitro propagation of Bacopa monnieri L.-a multipurpose plant. Indian J Sci Tech. 2010;3:781-6.

Vohora SB, Khanna T, Athar M. Analgesi of activity of Bacosine, a new triterpene isolated from Bacopa monniera. Fitoterapia. 1997;68:161-365.

Stough C, Lloyd J, Clarke J, Downey L, Hutchinson C, Rodgess T, Nathan P. The chronic effects of an extract of Bacopa monnieri (Brahmi) on cognitive function in healthy human subjects. Psychopharacol. 2001;156:481-4.

Basu,N, Walia K. The chemical investigations of the leaves of Herpestis monniera. Indian J Pharm. 1994;4:84-5.

Shakoor A, Akram M, Asharaf CM, Siddiqui MR. Pharmagonistic study and chemical / pharmacological evaluation of Brahmi-buti. Hamdard Medicus. 1994;37:92-109.

Tanvir A, Khan M, Shah F. In vitro micropropagation of Brahmi-Bacopa monnieri (L.) Pennell – A step for conservation. Nanobiotechnica Universale. 2010;1:139-50.

Jain J, Sharma V, Ramawat KG. Shoot culture of Bacopa monnieri: standardization of explant, vessels and bioreactor for growth and antioxidant capacity. Physiol Mol Biol Plants. 2012;18:185-90.

Rao S, Rajkumar P, Kaviraj C, Parveen PA. Efficient plant regeneration from leaf explants of Bacopa monnieri (L.) Wettst.: A threatened medicinal herb. Ann Phytomed. 2012;1:110-7.

Jain R, Prasad B, Jain M. In vitro regeneration of Bacopa monnieri (L.): A highly valuable medicinal plant. Int J Curr Microbiol App Sci. 2013;2:198-205.

Sharma B, Manohar SH, Majumdar M. Effect of phytohormones on leaf explants of Bacopa monnieri L. Penn: an endangered medicinal plant. Res J Pharm Biol Chem Sci. 2013;4:549-56.

Koul A, Sharma A, Gupta S, Mallubhotla S. Cost effective protocol for micropropagation of Bacopa monnieri using leaf explants. Int J Sci Res. 2014;3:210-2.

Schijlen E, Ric Devos CH, Jonker H, Broeck HVD, Molthoff J, Vantunen AV, Martens S, Bovy A. Pathway engineering for healthy phytochemicals leading to the production of novel flavonoids in tomato fruit. Plant Biotechnol J. 2006;4:433-44.

Dorais M, Ehret DL, Papadopoulos AP. Tomato (Solanum lycopersicum) health components, from the seed to the consumer. Phytochem Rev. 2008;7:231-50.

Shohael AM, Ali MB, Yu KW, Hahn EJ, Islam R, Paek KY. Effect of light on oxidative stress, secondary metabolites and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochem. 2006;41:1179-85.

Park SU, Ahn DJ, Jeon HJ, Kwon TR, Lim HS, Cjoi BS, Baek KH, Bae H. Increase in the contents of Ginsenosides in raw ginseng roots in response to 450 and 470 nm light from Light-Emitting Diodes. J Ginseng Res. 2012;36:198-204.

Gangadhar BH, Mishra RK, Pandian G, Park SW. Comparative study of color, pungency, and biochemical composition in chili pepper (Capsicum annuum) under different light-emitting diode treatments. HortScience. 2013;47:1729-35.

Jeong JH, Kim YS, Moon HK, Hwang SJ, Choi YE. Effects of LED on growth, morphogenesis and eleutheroside contents of in vitro cultured plantlets of Eleutherococcus senticosus Maxim. Kor J Med Crop Sci. 2009;17:39-45.

Kim K, Kook H, Jang J, Lee W, Kamala-Kannan S, Chae JC, Lee KJ. The effect of blue-light-emitting diodes on antioxidant properties and resistance to botrytis cinerea in tomato. J Plant Pathol Microbiol. 2013;4:203.

Lian ML, Murthy HN, Paek KY. Effects of light emitting diodes (LEDs) on the in vitro induction and growth of bulblets of Lilium oriental hybrid ‘Pesaro’. Sci Horti. 2002;94:365-70.

Huan LVT, Tanaka M. Effects of Red and Blue Light-Emitting Diodes on callus induction, callus proliferation, and protocorm-like body formation from callus in Cymbidium orchid. Environ Control Biol. 2004;42:57-64.

Rocha PSG, Oliveira RP, Scivittaro WB, Saints UL. Diodes emitting light and BAP concentrations in the multiplication in vitro of strawberry. Cienc Rural Santa Maria. 2010;40:1922-8.

Karataş M, Aasim, M. Efficient adventitious shoot regeneration of medicinal aquatic plant water hyssop (Bacopa monnieri L. Pennell). Pak J Agric Sci. 2014;51:665-670.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473-97.

Snedecor GW, Cochran WG. Statistical methods. Iowa, USA: The Iowa State University Press; 1997.

Mohapatra HP, Rath SP. In vitro studies of Bacopa monnieri-An important medicinal plant with reference to its biochemical variations. Ind J Exp Biol. 2005;43:373-6.

Karatas M, Aasim M, Dogan M, Khawar KM. Adventitious shoot regeneration of the medicinal aquatic plant water hyssop (Bacopa monnıeri L. PENNELL) using different internodes. Arch Biol Sci Belgrade. 2013;65:297-303.

Budiarto K. Spectral quality affects morphogenesis on Anthurium plantlet during in vitro culture. J Agr Sci. 2010;32:234-40.

Chung JP, Huang CY, Dai TE. Spectral effects on embryogenesis and plantlet growth of Oncidium ‘Gower Ramsey. Sci Horti. 2010;124:511-6.

Baque A, Shin YK, Elshmari T, Lee EJ, Paek KY. Effect of light quality, sucrose and coconut water concentration on the micropropagation of Calanthe hybrids (‘Bukduseong’ × ‘Hyesung’ and ‘Chunkwang’ × ‘Hyesung’). Aus J Crop Sci. 2011;5:1247-54.

Wu HC, DuToit ES. In vitro organogenesis of Protea cynaroides L. shoot-buds cultured under red and blue light-emitting diodes. In: Sato KI, editor. Embryogenesis. China: InTech; 2012. 151-66.

Li H, Xu Z, Tang C. Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro. Plant Cell Tiss Org Cult. 2010;103:155-63.

Chang HS, Charkabarty D, Hahn EJ, Paek KY. Micropropagation of calla lily (Zantedeschia albomaculata) via in vitro shoot tip proliferation. In Vitro Cell Dev Biol-Plant. 2003;39:129-34.

Kim SJ, Hahn EJ, Heo JW, Paek KY. Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hortic. 2004;110:143-51.

Stirk WA, Staden JV, Novak O, Dolezai K, Strnad M, Dobrev PI, Sipos G, Ördög A, Balint P. Changes in endogenous cytokinin concentrations in Chlorella (Chlorophyceae) in relation to light and the cell cycle. J Phycol. 2011;47:291-301.

Su W, Howell H. The effects of cytokinin and light on hypocotyl elongation in Arabidopsis seedlings are independent and additive. Plant Physiol. 1995;708:1423-30.

Goins GD, Yorio NC, Sanwo MM, Brown CS. Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with and without supplemental blue lighting. J Expt Bot. 1997;48:1407-13.

Devlin PF, Christie JM, Terry MJ. Many hands make light work. J Expt Bot. 2007;58:3071-7.


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