Response of Virginia (flue-cured) tobacco genotypes to water-deficit stress

Authors

  • Lydia Shtereva Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Elisaveta Stoimenova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Marina Drumeva-Yoncheva Tobacco and Tobacco Products Institute, Plovdiv
  • Bistra Michailova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Tania Kartzeva Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Roumiana Vassilevska-Ivanova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Keywords:

tobacco plant, drought stress, proline, hydrogen peroxide, lipid peroxidation

Abstract

The effect of prolonged water deficit on four Virginia (flue-cured) tobacco genotypes, Line 842, Oxford 207, RG11 and Virgin D, was analyzed in whole plants. Drought stress was induced by withholding irrigation and subjecting plants to low, moderate and severe regimes. Some growth indices such as fresh weight, plant growth rate, number, color and area of new developed leaves, as well as proline, hydrogen peroxide (H2O2) and malondialdehyde (MDA) content as a measure of oxidative stress were investigated to examine the role of genotype in water-deficit tolerance. Under stress, the weight of the aboveground parts of plants, plant growth height, number of new developed leaves and leaf area index decreased with the severity of treatment. The stressed plants accumulated more proline, malonildialdehide and hydrogen peroxide than control non-stressed plants under water-deficit conditions. The results showed that among the genotypes, Virgin D (VD) was the most sensitive to drought, while L 842 and Oxford 207 were moderately tolerant; RG11 was drought-tolerant. This suggests that the correlation between the physiological traits and level of antioxidative response exists and therefore it could be used as a rapid screening test to evaluate the drought tolerance of tobacco.

DOI: 10.2298/ABS160202085S

Received: February 2, 2016; Revised: May 27, 2016; Accepted: June 24, 2016; Published online: September 23, 2016

How to cite this article: Shtereva L, Stoimenova E, Drumeva-Yoncheva M, Michailova B, Kartzeva T, Vassilevska-Ivanova R. Response of Virginia (flue-cured) tobacco genotypes to water-deficit stress. Arch Biol Sci. 2017;69(1):119-27.

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Author Biographies

Lydia Shtereva, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Applied Genetics and Biotechnology

Assoc. Prof. Dr.

Elisaveta Stoimenova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Applied Genetics and Biotechnology

Prof.

Marina Drumeva-Yoncheva, Tobacco and Tobacco Products Institute, Plovdiv

Tobacco Breeding

Research Fellow

Bistra Michailova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Applied Genetics and Biotechnology

Research Fellow

Tania Kartzeva, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Applied Genetics and Biotechnology

Dr.

Roumiana Vassilevska-Ivanova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Applied Genetics and Biotechnology

Assoc. Prof.

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Published

2017-03-07

How to Cite

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Shtereva L, Stoimenova E, Drumeva-Yoncheva M, Michailova B, Kartzeva T, Vassilevska-Ivanova R. Response of Virginia (flue-cured) tobacco genotypes to water-deficit stress. Arch Biol Sci [Internet]. 2017Mar.7 [cited 2022Jan.19];69(1):119-27. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/103

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