Tryptophan enhances diet-induced improvement of white adipose tissue in obese rats
DOI:
https://doi.org/10.2298/ABS250902026YKeywords:
balanced diet, L-tryptophan, obesity, rats, white adipose tissueAbstract
Paper description:
- Dietary correction, a mainstay in obesity treatment, has limited effectiveness. L-tryptophan has shown potential in regulating lipid metabolism. This study investigated whether L-tryptophan enhances white adipose tissue (WAT) recovery during dietary correction in obese rats.
- Forty-eight Wistar rats were assigned to control, high-calorie diet, dietary correction, and dietary correction with L-tryptophan (80 mg/kg).
- L-tryptophan improved WAT morphology, reduced fibrosis, restored vascularization, and normalized lipid profiles better than diet alone, with changes in bioimpedance confirming improved tissue integrity.
- L-tryptophan can potentiate dietary obesity treatment, offering a promising adjunct strategy for restoring the morpho-functional state of WAT.
Abstract: The study examined the effect of L-tryptophan on white adipose tissue and its potential to enhance the effectiveness of fasting-dietary obesity correction. Forty-eight Wistar rats were divided into four groups: I – control; II – animals that received a high-calorie diet for 12 weeks; III – rats that after 12 weeks of a high-calorie diet received a balanced diet for 4 weeks; IV – rats that after 12 weeks of a high-calorie diet received a balanced diet with the addition of L-tryptophan (80 mg/kg body weight) for 4 weeks. The high-calorie diet contained 45% fat, 9% protein, and 31% carbohydrate. Physiological, morphological, histomorphometric, biochemical, and biophysical methods were used in the experiment. A high-calorie diet induced obesity and structural alterations in white adipose tissue in the rats. Dietary L-tryptophan accelerated restoration of adipocyte composition, decreased connective tissue, improved white adipose tissue vascularization, and normalized visceral fat mass and lipid metabolism markers. L-tryptophan also contributed to a reduction in the disturbance of the bioelectric properties of the white adipose tissue in obesity. This may indicate restoration of adipocyte membrane integrity, adipose tissue hydration, and microstructural organization. The presented information may be applied clinically in the comprehensive treatment of obesity.
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