Effects of troxerutin and selenium supplementation in mitigating cypermethrin-induced behavioral impairments and oxidative stress in mice
DOI:
https://doi.org/10.2298/ABS251107002SKeywords:
behavior, cypermethrin, neurotoxicity, oxidative stress, selenium, troxerutinAbstract
Paper description:
- Previous studies reported the neuroprotective effects of individual supplementation of troxerutin and selenium in terms of improved behavioral functions and redox status against cypermethrin-induced neurotoxicity in rodents.
- The co-supplementation effects of troxerutin and selenium have not been reported and are the primary focus of this study.
- A sub-acute mouse model was employed, administering cypermethrin along with single and combined supplementation of troxerutin and selenium.
- Single and co-supplementations alleviated behavioral deficits, oxidative stress, and normalized serum biomarkers.
Abstract: The current research aimed to investigate the potential effectiveness of single and combined supplementation with troxerutin (Trx) and selenium (Se) against cypermethrin (Cyp)-induced neurotoxicity and oxidative stress in mice. Thirty mice were randomly divided into five groups. The control group received 0.9% normal saline orally; the Cyp group received Cyp (5 mg/kg) orally; the Cyp+Trx group received Trx (150 mg/kg) in combination with Cyp; the Cyp+Se group received Se (25 µg/kg) with Cyp; and the Cyp+Trx+Se group received Trx (150 mg/kg) and Se (25 µg/kg) together with Cyp, all administered orally for 28 days. The individual and combined supplementation of Trx and Se significantly improved motor impairment, memory function, hepatic and renal health markers, and reduced anxiety levels (inside time/time in centar zone) in Cyp-exposed mice. Moreover, all supplementations reduced the Cyp-induced oxidative stress, but the SOD activity was increased in the serum and brain by the supplementation of Trx and Se, respectively. Liver and kidney SOD activities were improved with all the supplementations. Combined supplementation of Trx and Se enhanced brain acetylcholinesterase activity compared to the Cyp group. These findings suggest that both single and combined supplementation with Trx and Se confer neuroprotection against pesticide-induced oxidative and behavioral alterations; however, brain acetylcholinesterase activity and renal health markers were improved only by the combined Trx and Se supplementation.
Downloads
References
Namdev N, Rai G, Mahobiya P. Preventive role of dietary antioxidants against cypermethrin-induced toxicological effects: ashwagandha and quercetin. Goya J. 2023;16:325-34. https://doi.org/12.163022.Gj.2023.v16.05.0046
Ziada RM, Nahas A, Farag A, Kotb GA. Protective Efficacy of Combined Administration of Vitamins C and Curcumin on Cypermethrin-Induced Oxidative Stress in Male Albino Rats. Egypt Acad J Biol Sci F Toxicol Pest Control. 2020;12:109-18. https://doi.org/10.21608/eajbsf.2020.113976
Ali HF, El-Sayed NM, Ahmed AA, Hanna PA, Moustafa YM. Nano selenium ameliorates oxidative stress and inflammatory response associated with cypermethrin-induced neurotoxicity in rats. Ecotoxicol Environ Saf. 2020;195:110479. https://doi.org/10.1016/j.ecoenv.2020.110479
Ateşşahin A, Yilmaz S, Karahan I, Pirinçci I, Taşdemir B. The effects of vitamin E and selenium on cypermethrin-induced oxidative stress in rats. Turk J Vet Anim Sci. 2005;29:385-91. https://journals.tubitak.gov.tr/veterinary/vol29/iss2/30
Kalra S, Sangha G. Free radical mediated neurotoxicity induced by cypermethrin in rats. J Entomol Zool Stud. 2018;6:1087-92.
Panat NA, Maurya DK, Ghaskadbi SS, Sandur SK. Troxerutin, a plant flavonoid, protects cells against oxidative stress-induced cell death through radical scavenging mechanism. Food Chemist. 2016;194:32-45. https://doi.org/10.1016/j.foodchem.2015.07.078
Bayandor P, Farajdokht F, Mohaddes G, Diba R. Hosseindoost M, Mehri K, Zavvari Oskuye Z, Babri, S. The effect of troxerutin on anxiety-and depressive-like behaviours in the offspring of high-fat diet fed dams. Arch Physiol Biochem. 2019;125:156-62. https://doi.org/10.1080/13813455.2018.1443142
Lan ZF, Yao W, Xie YC, Chen W, Zhu YY, Chen JQ, Zhou XY, Huang JQ, Wu MS, Chen JX. Oral troxerutin alleviates Depression symptoms in mice by modulating gut microbiota and Microbial Metabolism. Mol Nutr Food Res. 2024;68:2300603. https://doi.org/10.1002/mnfr.202300603
Elangovan P, Pari L. Ameliorating effects of troxerutin on nickel-induced oxidative stress in rats. Redox Rep. 2013;18:224-32. https://doi.org/10.1179/1351000213Y.0000000055
Shehzad S, Farooq Z, Tahir S, Masood M, Anjum S, Saeed A, Yousaf M, Majeed K, Rabbani I, Basharat S. Neuro-protective and redox potential of troxerutin against cypermethrin-induced neurotoxicity and oxidative stress in mice. Pol J Vet Sci. 2024;289-99. https://doi.org/10.24425/pjvs.2024.149362
Ozturk Kurt B, Ozdemir S. Selenium heals the chlorpyrifos-induced oxidative damage and antioxidant enzyme levels in the rat tissues. Biol Trace Elem Res. 2023;201:1772-80. https://doi.org/10.1007/s12011-022-03271-x
Kieliszek M. Selenium–fascinating microelement, properties and sources in food. Molecules. 2019;24:1298. https://doi.org/10.3390/molecules24071298
Sharma P, Firdous S, Singh R. Neurotoxic effect of cypermethrin and protective role of resveratrol in Wistar rats. Int J Nutr Pharmacol Neurol Dis. 2014;4:104-11. https://doi.org/10.4103/2231-0738.129598
Feldman E. Thiobarbituric acid reactive substances (TBARS) assay [protocol] [Internet]. Springer Nature; 2019 [2019 Aug 9]. https://dx.doi.org/10.17504/protocols.io.3sngnde
Li X. Improved pyrogallol autoxidation method: a reliable and cheap superoxide-scavenging assay suitable for all antioxidants. J Agric Food Chem. 2012;60:6418-24. https://doi.org/10.1021/jf204970r
Hadwan MH, Abed HN. Data supporting the spectrophotometric method for the estimation of catalase activity. Data Brief. 2016;6:194-9. https://doi.org/10.1016/j.dib.2015.12.012
Ellman GL, Courtney KD, Andres JrV, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961;7:88-95. https://doi.org/10.1016/0006-2952(61)90145-9
Nieradko-Iwanicka B, Borzęcki A. Influence of fenpropathrin on memory and movement in mice after transient incomplete cerebral ischemia. J Toxicol Environ Health Part A. 2010;73:1166-72. https://doi.org/10.1080/15287394.2010.491411
Nasuti C, Brunori G, Eusepi P, Marinelli L, Ciccocioppo R, Gabbianelli R. Early life exposure to permethrin: a progressive animal model of Parkinson's disease. J Pharmacol Toxicol Meth. 2017;83:80- 6. https://doi.org/10.1016/j.vascn.2016.10.003
Nieradko-Iwanicka B, Borzêcki A. Effect of cypermethrin on memory, movement activity and co-ordination in mice after transient incomplete cerebral ischemia. Pharmacol Rep. 2008;1;60(5):699.
Garabadu D, Agrawal N. Naringin exhibits neuroprotection against rotenone-induced neurotoxicity in experimental rodents. Neuromol Med. 2020;22:314-30. https://doi.org/10.1007/s12017-019-08590-2
Hill KE, McMahan W, Motley AK, Burk RF, Zhou J. Neurological dysfunction occurs in mice with targeted deletion of the selenoprotein P gene. J Nutr. 2004;134:157-161. https://doi.org/10.1093/jn/134.1.157
Latuszyńska J, Luty S, Raszewski G, Tokarska-Rodak M, Przebirowska D, Przylepa E, Haratym-Maj A. Neurotoxic effect of dermally-applied chlorpyrifos and cypermethrin in Wistar rats. Ann Agric Environ Med. 2001;8(2):163-70.
Yadav S, Das RS, Prakash S. Effect Of Pyrethroid Pesticides On Behaviour Of Adult Mice. Int J Med Sci Curr Res. 2022;5:1079.
Tavakkoli M, Dadkhah M, Saadati H, Afshari S, Mostafalou S. Neurobehavioral toxicity of cypermethrin in association with oxidative, inflammatory and neurotrophic changes in the hippocampus of rats. Int J Environ Health Res. 2025;35(12):3911-22. https://doi.org/10.1080/09603123.2025.2503472
Zhang ZH, Peng JY, Chen YB,Wang C, Chen C, Song GL. Different effects and mechanisms of selenium compounds in improving pathology in Alzheimer’s disease. Antioxidants. 2013;12:702. https://doi.org/10.3390/antiox12030702
Sowunmi AA, Omeiza NA, Bakre A, Abdulrahim HA, Aderibigbe AO. Dissecting the antidepressant effect of troxerutin: modulation of neuroinflammatory and oxidative stress biomarkers in lipopolysaccharide-treated mice. Naunyn Schmied. Arch Pharmacol. 2024;397:9965-79. https://doi.org/10.1007/s00210-024-03252-y
Narwanto MI, Purwandhono A, Sofiana KD, Febianti Z, Putri ERM, Jauhani MA. Neurotoxicity of Chlorpyrifos, Carbofuran, and Cypermethrin in Adolescent Rats’ Brain. Maj Kedokt Bdg. 2022;54:202-7. https://doi.org/10.15395/mkb.v54n4.2749
Jamali-Raeufy N, Kardgar S, Baluchnejadmojarad T, Roghani M, Goudarzi M. Troxerutin exerts neuroprotection against lipopolysaccharide (LPS) induced oxidative stress and neuroinflammation through targeting SIRT1/SIRT3 signaling pathway. Metab Brain Dis. 2019;34:1505-13. https://doi.org/10.1007/s11011-019-00454-9
Seven B, Kültiğin, Çavuşoğlu, Yalçin E, Aca A.Investigation of cypermethrin toxicity in Swiss albino mice with physiological, genetic and biochemical approaches. Sci Rep. 2022;12:11439. https://doi.org/10.1038/s41598-022-15800-8
Abdou HM, Hussien HM, Yousef MI. Deleterious effects of cypermethrin on rat liver and kidney: protective role of sesame oil. J Environ Sci Health, Part B. 2012;47:306-14. https://doi.org/10.1080/03601234.2012.640913
Ozturk Kurt B, Konukoglu D, Kalayci R, Ozdemir S. Investigation of the protective role of selenium in the changes caused by chlorpyrifos in trace elements, biochemical and hematological parameters in rats. Biol Trace Elem Res. 2020;200:228-237. https://doi.org/10.1007/s12011-021-02616-2
Yarahmadi S, Mehrjoo M, Asgharzadeh S, Pakravan S, Ahmadvand H, Babaeenezhad E. The combination effects of sodium selenite and vitamin E on renal ischemia-reperfusion injury in rats. J Renal Inj Prev. 2024;14:e38400. https://doi.org/10.34172/jrip.2025.38400
Ince S, Kucukkurt I, Demirel, HH, Turkmen R, Sever E. Thymoquinone attenuates cypermethrin induced oxidative stress in swiss albino mice. Pestic Biochem Physiol. 2012;104:229-35. https://doi.org/10.1016/j.pestbp.2012.09.003
Messaoudi I, El Heni J, Hammouda F, Saïd K, Kerkeni A. Protective effects of selenium, zinc, or their combination on cadmium-induced oxidative stress in rat kidney. Biol Trace Elem Res. 2009;130:152-61. https://doi.org/10.1007/s12011-009-8324-y
Ighodaro O, Akinloye O. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alex J Med. 2018;54(4):287-93. https://doi.org/10.1016/j.ajme.2017.09.001
Rossi MJ, Pekkurnaz G. Powerhouse of the mind: mitochondrial plasticity at the synapse. Curr Opin Neurobiol. 2019; 57:149-55. https://doi.org/10.1016/j.conb.2019.02.001
del-Rahman A, Dechkovskaia AM, Goldstein LB, Bullman SH, Khan W, El-Masry EM, Abou-Donia MB. Neurological deficits induced by malathion, DEET, and permethrin, alone or in combination in adult rats. J Toxicol Environ Health Part A. 2004;67:331-56. https://doi.org/10.1080/15287390490273569
Adedara IA, Fabunmi AT, Ayenitaju FC, Atanda OE, Adebowale AA, Ajayi BO, Owoeye O, Rocha JB, Farombi EO. Neuroprotective mechanisms of selenium against arsenic-induced behavioral impairments in rats. Neurotoxicol. 2020;76:99-110. https://doi.org/10.1016/j.neuro.2019.10.009
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Sobia Shehzad, Sajid Khan Tahir, Muhammad Shahbaz Yousaf, Muhammad Irfan Masood, Syed Muhammad Muneeb Anjum, Imad Khan
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.
