IMPACT OF APPLICATION OF ZINC OXIDE NANOPARTICLES ON CALLUS INDUCTION, PLANT REGENERATION, ELEMENT CONTENT AND ANTIOXIDANT ENZYME ACTIVITY IN TOMATO (SOLANUM LYCOPERSICUM MILL.) UNDER SALT STRESS

Authors

  • Hesham F. Alharby Department of Biological Sciences, Faculty of Science, King Abdulaziz University, 21589 Jeddah
  • Ehab M.R. Metwali 1. Biological Science Department, Faculty of Science, University of Jeddah, 21589 Jeddah, Saudi Arabia; 2. Botany Department, Faculty of Agriculture, Suez Canal University, 41522 Ismailia, Egypt
  • Michael P. Fuller School of Biological Sciences, Faculty of Science and Engineering, Plymouth University, Plymouth, PL4 8AA
  • Amal Y. Aldhebiani Department of Biological Sciences, Faculty of Science, King Abdulaziz University, 21589 Jeddah

Abstract

The properties of nanomaterials and their potential applications have been given considerable attention by researchers in various fields, especially agricultural biotechnology. However, not much has been done to evaluate the role or effect of zinc oxide nanoparticles (ZnO-NPs) in regulating physiological and biochemical processes in response to salt-induced stress. For this purpose, some callus growth traits, plant regeneration rate, mineral element (sodium, potassium, phosphorous and nitrogen) contents and changes in the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in tissues of five tomato cultivars were investigated in a callus culture exposed to elevated concentrations of salt (3.0 and 6.0 g L-1NaCl), and in the presence of zinc oxide nanoparticles (15 and 30 mg L-1). The relative callus growth rate was inhibited by 3.0 g L-1 NaCl; this was increased dramatically at 6.0 g L-1. Increasing exposure to NaCl was associated with a significantly higher sodium content and SOD and GPX activities. Zinc oxide nanoparticles mitigated the effects of NaCl, and in this application of lower concentrations (15 mg L-1) was more effective than a higher concentration (30 mg L-1). This finding indicates that zinc oxide nanoparticles should be investigated further as a potential anti-stress agent in crop production. Different tomato cultivars showed different degrees of tolerance to salinity in the presence of ZnO-NPs. The cultivars Edkawy, followed by Sandpoint, were less affected by salt stress than the cultivar Anna Aasa.

DOI: 10.2298/ABS151105017A

Key words: Nano biotechnology, in vitro culture, protein, salinity, superoxide dismutase, glutathione peroxidase

Received: November 5, 2015; Revised: February 11, 2016; Accepted: February 12, 2016; Published online: March 21, 2016

How to cite this article: Alharby HF, Metwali EMR, Fuller MP, Aldhebiani AY. Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress. Arch Biol Sci. 2016;68(4):723-35.

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Published

2016-11-25

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Alharby HF, Metwali EM, Fuller MP, Aldhebiani AY. IMPACT OF APPLICATION OF ZINC OXIDE NANOPARTICLES ON CALLUS INDUCTION, PLANT REGENERATION, ELEMENT CONTENT AND ANTIOXIDANT ENZYME ACTIVITY IN TOMATO (SOLANUM LYCOPERSICUM MILL.) UNDER SALT STRESS. Arch Biol Sci [Internet]. 2016Nov.25 [cited 2022May29];68(4):723-35. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1244

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