GLUCOSE-INDUCED LIPID DEPOSITION IN GOOSE PRIMARY HEPATOCYTES IS DEPENDENT ON THE PI3K-Akt-mTOR SIGNALING PATHWAY

Authors

  • Chunchun Han Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Shouhai We College of Life Science, Sichuan Agricultural University, Ya’an, Sichuan, 625014
  • Fang He Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Qi Song Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Xiangping Xiong Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Fengjiang Ye Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Dandan Liu Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Huofu Wan Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Hehe Liu Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Liang Li Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Hongyong Xu Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Feng Xu Institute of Animal Breeding and Genetics, Sichuan Agricultural University, Chengdu, Sichuan, 61130
  • Xianyin Zeng College of Life Science, Sichuan Agricultural University, Ya’an, Sichuan, 625014

Abstract

Previously we showed that fatty liver formation in overfed geese was accompanied by PI3K-Akt-mTOR pathway activation and changes in plasma glucose concentrations. Here, we show that glucose acts in goose hepatocellular lipid metabolism through the PI3K-Akt-mTOR signaling pathway. We observed that glucose increased lipogenesis, decreased fatty acid oxidation and increased very low density lipoprotein triglyceride (VLDL-TG) assembly and secretion. Co-treatment with glucose and inhibitors of the PI3K-Akt-mTOR pathway (LY294002, rapamycin, NVP-BEZ235) decreased the levels of factors involved in lipogenesis and increased the levels of factors involved in fatty acid oxidation and VLDL-TG assembly and secretion. These findings show that inhibition of the PI3K-Akt-mTOR pathway decreased glucose-induced lipogenesis, inhibited the downregulation of fatty acid oxidation by glucose and increased the upregulation of VLDL-TG assembly and secretion by glucose. The results presented herein provide further support for the role of the PI3K-Akt-mTOR pathway in lipid metabolism as we showed that in goose primary hepatocytes, glucose acts through the PI3K-Akt-mTOR-dependent pathway to stimulate lipid deposition by increasing lipogenesis and decreasing fatty acid oxidation and VLDL-TG assembly and secretion.

DOI: 10.2298/ABS151210075H

Key words: glucose; PI3K-Akt-mTOR pathway; lipogenesis; fatty acid oxidation; VLDL assembly and secretion

Received: December 10, 2015; Revised: January 15; Accepted: January 16, 2016; Published online: August 19, 2016

How to cite this article: Han C, We S, He F, Qi S, Xiong X, Ye F, Liu D, Wan H, Liu H, Li L, Xu H, Xu F, Zeng X. Glucose-induced lipid deposition in goose primary hepatocytes is dependent on the PI3K-Akt-mTOR signaling pathway. Arch Biol Sci. 2016;68(4):853-61.

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Published

2016-11-25

How to Cite

1.
Han C, We S, He F, Song Q, Xiong X, Ye F, Liu D, Wan H, Liu H, Li L, Xu H, Xu F, Zeng X. GLUCOSE-INDUCED LIPID DEPOSITION IN GOOSE PRIMARY HEPATOCYTES IS DEPENDENT ON THE PI3K-Akt-mTOR SIGNALING PATHWAY. Arch Biol Sci [Internet]. 2016Nov.25 [cited 2022May29];68(4):853-61. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1260

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