Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India


  • Venkataravanappa Venkataravanappa Central Horticultural Experiment Station (CHES), Chettalli – 571248, Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore
  • Lakshminarayana Reddy C Narasimha Reddy Department of Plant Pathology, College of Sericulture, Chintamani – 563125, University of Agricultural Sciences (B), Karnataka
  • Sujoy Saha Division of Plant Pathology, National Research Centre for Grapes, Pune – 412307, Maharashtra
  • Shankarappa K Subbanna Department of Plant Pathology, College of Horticulture, Bengaluru-560065, University of Horticultural Sciences, Bagalkot, Karnataka
  • Krishna Reddy Manem Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bengaluru-560089, Karnataka


begomoviruses, ivy gourd, recombination, phylogenetic analysis, perennial crop


Sixteen ivy gourd (Coccinia grandis (L.) Voigt) plant samples showing severe mosaic symptoms were collected from New Delhi and Varanasi (Uttar Pradesh) in India. Begomovirus infection was confirmed by PCR using begomovirus-specific primers. Amplified PCR products (1.2 kb fragments) were cloned and the sequence was characterized. Based on sequence analysis, begomovirus associated with the majority of ivy gourd samples (16) was found to be a member of a bipartite begomovirus species, which is closely related to tomato leaf curl New Delhi virus (ToLCNDV). Therefore, two samples of ivy gourd, IVG1-ND and IVG2-Var, were selected for full-length genome (DNA-A and DNA-B-like sequence) amplification by the rolling circle DNA amplification (RCA) method. Sequence analysis performed using the Species Demarcation Tool (SDT) program revealed that they share 89.5-91.3% (IVG1-ND) and 93.4-96.8% (IVG2-Var) nucleotide (nt) identity with the DNA-A-like sequence of ToLCNDV isolated from cucurbits and chilli, respectively. The IVG1-ND and IVG2-Var isolates shared 90% nt identity among themselves, indicating that they are two different strains of ToLCNDV. Similarly, SDT analysis of the DNA-B-like sequence of IVG1-ND and IVG2-Var exhibited showed 82.7-93.3% nt identity with the DNA-B-like sequences of ToLCNDV infecting cucurbits. The recombination analysis of DNA-A and DNB-B-like sequences showed that the greater part of their genome most likely originated from previously reported begomoviruses that are known to infect chilli and cucurbits through recombination.

Received: June 16, 2017; Revised: November 16, 2017; Accepted: November 29, 2017; Published online: December 21, 2017

How to cite this article: Venkataravanappa V, Narasimha Reddy LRC, Saha S, Subbanna SK, Manem KR. Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India. Arch Biol Sci. 2018;70(2):339-47.


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Author Biography

Shankarappa K Subbanna, Department of Plant Pathology, College of Horticulture, Bengaluru-560065, University of Horticultural Sciences, Bagalkot, Karnataka


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How to Cite

Venkataravanappa V, Narasimha Reddy LRC, Saha S, Subbanna SK, Manem KR. Detection and characterization of tomato leaf curl New Delhi virus association with mosaic disease of ivy gourd (Coccinia grandis (L.) Voigt) in North India. Arch Biol Sci [Internet]. 2018May30 [cited 2022Sep.29];70(2):339-47. Available from:




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