Therapeutic effects of polysaccharides extracted from Porphyra yezoensis in rats with cerebral ischemia/reperfusion injury
Keywords:Porphyra yezoensis, neurological deficit, cerebral infarct, oxidative stress
The polysaccharides of Porphyra yezoensis (PPY), porphyrans, are recognized as the major active components as they have several biological properties. This study was performed to assess the effect of PPY administration against cerebral ischemia-reperfusion injury (IRI). Thirty-two adult Sprague-Dawley (SD) rats were chosen and divided into 4 groups as follows: group I – rats received only saline; group II – subjected to middle cerebral artery occlusion (MCAO) for 60 min followed by 24 h of reperfusion (IRI-induced); group III – pretreated with PPY (100 mg/kg) for 7 days, followed by IRI induction; group IV – treated with PPY (100 mg/kg) for 7 days without IRI induction. All the data were analyzed by Dunnett’s (multiple comparisons) test using SPSS software. Pretreatment with PPY significantly (p<0.01) lowered the neurological deficit and cerebral infarct volume in comparison with IRI-induced rats. A pronounced (p<0.01) increase in the levels of antioxidant components (superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH)) was observed in PPY-supplemented rats. Also, the proinflammatory markers: interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α) and the nuclear factor-kappa B protein, subunit p65 (NF-κB p65) were substantially (p<0.01) suppressed in PPY-administered rats. Moreover, the protein levels of TNF-α and NF-κB p65 were considerably (p<0.01) downregulated upon pretreatment with PPY. Our data suggest that PPY could exhibit neuroprotective activity by attenuating oxidative stress and the inflammatory response.
Received: June 21, 2017; Revised: October 8, 2017; Accepted: October 8, 2017; Published online: October 16, 2017
How to cite this article: Sun C, Wu F, Chen D, Ge J. Therapeutic effects of polysaccharides extracted from Porphyra yezoensis in rats with cerebral ischemia/reperfusion injury. Arch Biol Sci. 2018;70(2):233-9.
Ai J, Wan H, Shu M, Zhou H, Zhao T, Fu W, He Y. Guhong injection protects against focal cerebral ischemia-reperfusion injury via anti-inflammatory effects in rats. Arch Pharm Res. 2017;40(5):610-22.
Yu X, Zhou C, Yang H, Huang X, Ma H, Qin X Hu J. Effect of ultrasonic treatment on the degradation and inhibition cancer cell lines of polysaccharides from Porphyra yezoensis. CarbohydrPolym. 2015;117:650-6.
Khatri R, McKinney AM, Swenson B, Janardhan V. Blood-brain barrier, reperfusion injury, and hemorrhagic transformation in acute ischemic stroke. Neurol. 2012;79(13):S52-S57.
Radenović L, Selaković V, Anđus PR. Neuroprotectionby MK-801 following cerebral ischemia in Mongolian gerbils. Arch Biol Sci. 2008;60(3):341-6.
Chen Y, Li Y, Xu H, Li G, Ma Y, Pang YJ. Morin mitigates oxidative stress, apoptosis and inflammation in cerebral ischemic rats. Afr J Tradit Complement Altern Med. 2017;14(2):348.
Yan J, Zheng M, Zhang D. Chrysophanol liposome preconditioning protects against cerebral ischemia-reperfusion injury by inhibiting oxidative stress and apoptosis in mice. Int J Pharmacol. 2014;10(1):55-68.
Niwa K, Iida S, Kato A, Kawai H, Kikuchi N, Kobiyama A, Aruga Y. Genetic diversity and introgression in two cultivated species (Porphyra yezoensis and Porphyra tenera) and closely related wild species of Porphyra (Bangiales, Rhodophyta). J Phycol. 2009;45(2):493-502.
Lee HA, Kim IH, Nam TJ. Bioactive peptide from Pyropia yezoensis and its anti-inflammatory activities. Int J Mol Med. 2015;36(6):1701-6.
Zhang LX, Cai CE, Guo TT, Gu JW, Xu HL, Zhou Y, Wang Y, Liu CC,He PM. Anti-cancer effects of polysaccharide and phycocyanin from Porphyra yezoensis. J Mar Sci Tech. 2011;19(4):377-82.
Zhou C, Yu X, Zhang Y, He R, Ma H. Ultrasonic degradation, purification and analysis of structure and antioxidant activity of polysaccharide from Porphyra yezoensis udea. Carbohydr. Polym. 2012;87(3):2046-51.
Guo TT, Xu HI, Zhang LX, Zhang JP, Guo YF, Gu JW, He PM. In vivo protective effect of Porphyra yezoensis polysaccharide against carbon tetrachloride induced hepatotoxicity in mice. Regul Toxicol Pharmacol. 2007;49(2):101-6.
Qian L, Zhou Y, Ma JX. Hypolipidemic effect of the polysaccharides from Porphyra yezoensis. Int J Biol Macromol. 2014;68:48-49.
Xie Y, Zhang B, Zhang Y. Protective effects of Acanthopanax polysaccharides on cerebral ischemia–reperfusion injury and its mechanisms. Int J Biol Macromol. 2015;72:946-50.
Wang T, Li Y, Wang Y, Zhou R, Ma L, Hao Y, Jin S, Du J, Zhao C, Sun T. Lycium barbarum polysaccharide prevents focal cerebral ischemic injury by inhibiting neuronal apoptosis in mice. PloS One. 2014;9(3):e90780.
Longa EZ, Weinstein PR, Carlson S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 1989;20(1):84-91.
Türeyen K, Vemuganti R, Sailor KA, Dempsey RJ. Infarct volume quantification in mouse focal cerebral ischemia: A comparison of triphenyltetrazolium chloride and cresyl violet staining techniques. J Neurosci Met. 2004;139(2):203-207.
Wu S, Hao J, Gao W, Zhou B, Man S, Huang L. Neuroprotective effect and chemical composition of traditional Chinese medicines, zha-chong-shi-san-wei pill, against ischemic stroke on middle cerebral ischemia occlusion in rats. Lat Am J Pharm. 2013;32(7):1020-6.
Gong G, Xiang L, Yuan L, Hu L, Wu W, Cai L Yin L, Dong H. Protective effect of glycyrrhizin, a direct hmgb1 inhibitor, on focal cerebral ischemia/reperfusion-induced inflammation, oxidative stress, and apoptosis in rats. PLoS One. 2014;9(3):e89450.
Zhou C, Wang Y, Ma H, He R. Effect of ultrasonic degradation on in vitro antioxidant activity of polysaccharides from Porphyra yezoensis (rhodophyta). Revista De Agaroquimicay Tecnologia De Alimentos. 2008;14(6):479-86.
Liu F, Liu J, Gu J, Zhang L, Shen W, Guo T, Liu C, He P. Ex vivo anti-oxidation activity of polysaccharides from red alga Porphyra yezoensis. Res J Pharmacol. 2007;1(3):61-6.
Mohibbullah M, Bhuiyan MMH, Hannan MA, Getachew P, Hong YK, Choi JS, Choi TS, Moon IS. The edible red alga Porphyra yezoensis. Cell Mol Neurobiol. 2016;36(5):669-82.
Ryu J, Kwon MJ, Nam TJ. Nrf2 and nf-κb signaling pathways contribute to porphyra-334-mediated inhibition of uva-induced inflammation in skin fibroblasts. Marine Drugs. 2015;13(8):4721-32.
YangY, Liu P, Chen L, Liu Z, Zhang H, Wang J, Sun X, Zhong W, Wang N, Tian K. Therapeutic effect of Ginkgo biloba polysaccharide in rats with focal cerebral ischemia/reperfusion (i/r) injury. Carbohydr Polym. 2013;98(2):1383-8.
Jiang Z, Hama Y, Yamaguchi K, Oda T. Inhibitory effect of sulphated polysaccharide porphyran on nitric oxide production in lipopolysaccharide-stimulated raw264. 7 macrophages. J Biochem. 2011;151(1):65-74.