20-Hydroxyecdysone protects wheat seedlings from salt stress
Keywords:Triticum aestivum, 20-hydroxyecdysone, salt stress, oxidative stress, antioxidative system
20-Hydroxyecdysone (20E), a molting hormone of insects, is the most abundant phytoecdysteroid (PE) produced by plants, where it represents a protective molecule against insect herbivores. The objective of the investigation is to determine the effect of 20E on the growth, physiological and biochemical characteristics and the transcript levels of antioxidant enzymes of wheat seedlings under salt stress. Our results showed that exogenous 20E was able to significantly alleviate salt-induced oxidative damage in wheat seedlings. It most likely acts by decreasing the concentration of malondialdehyde (MDA) and the rate of superoxide radical (O2•−) generation, and by increasing the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), concentrations of ascorbic acid (AsA) and glutathione (GSH), and the gene expression levels of SOD, POD, CAT. It was suggested that foliar spraying with 20E could efficiently protect wheat seedlings against salt-induced oxidative stress. The results also show that 20E had a positive physiological effect on the growth of salt-stressed seedlings. This is the first report dealing with the effect of 20E pretreatment in the enhancing of wheat seedling tolerance under salt stress.
Received: July 22, 2017; Revised: November 14, 2017; Accepted: December 11, 2017; Published online: December 22, 2017
How to cite this article: Li J, Han X, Tang L, Wang C, Zhang W, Ma J. 20-hydroxyecdysone protects wheat seedlings from salt stress. Arch Biol Sci. 2018;70(2):379-86.
Hu J, Zhao TZ, Chu WH, Luo CX, Tang H, Yi L, Feng H. Protective effects of 20-hydroxyecdysone on CoCl2-induced cell injury in PC12 cells. J Cell Biochem. 2010;111:1512-21.
Holá D, Kočová M, Rothová O, Tůmová L, Kamlar M, Macek T. Exogenously applied 20-hydroxyecdysone increases the net photosynthetic rate but does not affect the photosynthetic electron transport or the content of photosynthetic pigments in Tetragonia tetragonioides L. Acta Physiol Plant. 2013;35:3489-95.
Rothová O, Holá D, Kočová M, Tůmováa M, Hniličkab F, Hniličkováb H, Kamlarc M, Macekc T. 4-Epibrassinolide and 2-hydroxyecdysone affect photosynthesis differently in maize and spinach[J]. Steroids. 2014;85:44-57.
Golovatskaya IF. Effect of ecdysterone on morphological and physiological processes in plants. Russ J Plant Physiol. 2004;51:407-13.
Bakrim A, Lamhamdi M, Sayah F, Chibi F. Effects of plant hormones and 20-hydroxyecdysone on tomato (Lycopersicum esculentum) seed germination and seedlings growth. Afr J Biotech. 2009;6:2792-802.
Haitov B, Mamadalieva N, Egamberdieva D. Plant derived 20-hydroxyecdysone alleviates salt stress in cotton (Gossypium hirsutum L.) seedlings. Asian Australasian J Plant Sci Biotechnol. 2013;7:39-42.
Hu J, Luo CX, Chu WH, Shan YA, Qian ZM, Zhu G, Yu YB, Feng H. 20-Hydroxyecdysone protects against oxidative stress-induced neuronal injury by scavenging free radicals and modulating NF-kB and JNK pathways. PLoS One. 2012;12:e50764.
Lamhamdi M, Lafont R, Rharrabe K, Sayah F, Aarab A, Bakrim A. 20-Hydroxyecdysone protects wheat seedlings (Triticum aestivum L.) against lead stress. Plant Physiol Bioch. 2016;98:64-71.
Li JT, Qiu ZB, Zhang XW, Wang LS. Exogenous hydrogen peroxide can enhance tolerance of wheat seedlings to salt stress. Acta Physiol Plant. 2011;33:835-42.
Wu HF, Liu XL, You LP, Zhang LB, Zhou D, Feng JH. Effects of salinity on metabolic profiles, gene expressions, and antioxidant enzymes in Halophyte Suaeda salsa. J Plant Growth Regul. 2012;31:332-41.
Talaat NB. Effective microorganisms improve growth performance and modulate the ROS-scavenging system in common bean (Phaseolus vulgaris L.) plants exposed to salinity stress. J Plant Growth Regul. 2015;34:35-46.
Triantaphylides C, Havaux M. Singlet oxygen in plants: production, detoxification and signaling. Trends Plant Sci. 2009;14:219-28.
Gao LM, Li YF, Han R. He-Ne laser preillumination improves the resistance of tall fescue (Festuca arundinacea Schreb.) seedlings to high saline conditions. Protoplasma. 2015;252:1135-48.
Zhang ZP, Miao MM, Wang CL. Effects of ALA on photosynthesis, antioxidant enzyme activity, and gene expression, and regulation of proline accumulation in tomato seedlings under NaCl stress. J Plant Growth Regul. 2015;34:637-50.
Predieri S, Norman HA, Krizek DT, Pillai P, Mirecki RM, Zimmerman RH. Influence of UV-B radiation on membrane lipid composition and ethylene of evolution in ‘Doyenne d’Hiver’ pear shoots grown in vitro under different photosynthetic photo fluxes. Environ Exp Bot. 1995;35:151-60.
Sergiev I, Alexieva V, Karanov E. Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. CR Acad Bulg Sci. 1997;51:121-4.
Elstner EF, Heupel A. Inhibition of nitrite formation from hydro-xylaminonium-chloride: a simple assay for superoxide dismutase. Anal Biochem. 1976;70:616-20.
Giannopolitis CN, Ries SK. Superoxide dismutaseⅠoccurrence in higher plants. Plant Physiol. 1977;59:309-14.
Cakmak I, Marschner H. Magnesium deficiency and high light intensity on enhance activities of superoxide dismutase, peroxidase and glutatione reductase in bean leaves. Plant Physiol. 1992;98:1222-7.
Zhang JX, Kirham MB. Drought stress-induced changes in activities of superoxide dismutase, catalase and peroxidase in wheat species. Plant Cell Physiol. 1994;35:785-91.
Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981;22:867-80.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82:70-7.
Tonamura B, Nakatani H, Ohnishi M, Yamaguchiito J, Hiromi K. Test reactions for a stopped flow apparatus regulation of 2, 6-D and potassium ferricynide by L-ascorbic acid. Anal Biochem. 1978;84:370-83.
Bradford M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
Qiu ZB, Li JT, Zhang MM, Bi ZZ, Li ZL. He-Ne laser pretreatment protects wheat seedlings against cadmium-induced oxidative stress. Ecotox Environ Safe. 2013;88:135-41.
Tijen D, Ismail T. Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environ Exp Bot. 2005;53:247-57.
Yasmeen A, Basra SMA, Farooq M, Rehman H, Hussain NY, Athar HUR. Exogenous application of moringa leaf extract modulates the antioxidant enzyme system to improve wheat performance under saline conditions. Plant Growth Regul. 2013;69:225-33.
Bajguz A, Godlewska-Zylkiewicz B. Protective role of 20-hydroxyecdysone against lead stress in Chlorella vulgaris cultures. Phytochemistry. 2004;65:711-20.
Cao S, Xu Q, Cao Y, Qian K, An K, Zhu Y. Loss of function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis. Physiol Plantarum. 2005;123:57-66.