The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious

Authors

Keywords:

cefotaxime, meropenem, Malus × domestica Borkh., regeneration, timentin

Abstract

Since the genetic transformation of the apple is strongly genotype-dependent and generally inefficient, the evaluation of factors affecting shoot regeneration are crucial for the establishment of a successful transformation process. In this report, we evaluated the effects of the β-lactam antibiotics meropenem and timentin on in vitro regeneration via de novo shoot organogenesis from leaf explants of apple cv. Golden Delicious, as well as on the growth of the Agrobacterium tumefaciens strain EHA 105, and compared them with the commonly used β-lactam cefotaxime. Also, we report for the first time the effect of hygromycin B as a selective agent in the domesticated apple, as regards shoot regeneration and shoot multiplication efficiency. We observed that cefotaxime and timentin at concentrations higher than 100 mg L-1 were sufficient to prevent Agrobacterium growth during a two-week period, while meropenem exhibited an inhibitory effect on bacterial growth at all tested concentrations (25-150 mg L-1). Cefotaxime at a concentration of 300 mg L-1 increased the number of regenerated shoots per explant (9.39) in comparison with the control (7.67). In contrast to cefotaxime, meropenem and timentin caused a decrease in shoot regeneration efficiency, but larger and more developed shoots were obtained on meropenem (25-125 mg L-1) after the same period of cultivation. Hygromycin B at a concentration of 5 mg L-1 or higher completely inhibited shoot regeneration and induced explant tissue necrosis. Therefore, the selection procedure with a final concentration of 4 mg L-1 throughout organogenesis and 10 mg L-1 for further shoot growth and multiplication is recommended for an efficient transformation process in apple cv. Golden Delicious.

https://doi.org/10.2298/ABS170731037S

Received: July 31, 2017; Revised: September 23, 2017; Accepted: September 25, 2017; Published online: October 11, 2017

How to cite this article: Stanišić M, Ninković S, Savić J, Ćosić T, Mitić N. The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious. Arch Biol Sci. 2018;70(1):179-90.

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Author Biographies

Mariana Stanišić, Institute for Biological Research "Siniša Stanković", University of Belgrade

Department of Plant Physiology

Slavica Ninković, Institute for Biological Research "Siniša Stanković", University of Belgrade

Department of Plant Physiology

Jelena Savić, Institute for Biological Research "Siniša Stanković", University of Belgrade

Department of Plant Physiology

Tatjana Ćosić, Institute for Biological Research "Siniša Stanković", University of Belgrade

Department of Plant Physiology

Nevena Mitić, Institute for Biological Research "Siniša Stanković", University of Belgrade

Department of Plant Physiology

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Published

2018-03-13

How to Cite

1.
Stanišić M, Ninković S, Savić J, Ćosić T, Mitić N. The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. Golden Delicious. Arch Biol Sci [Internet]. 2018Mar.13 [cited 2024Mar.29];70(1):179-90. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2022

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