The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage

Authors

  • Gulsah Yildiz Deniz Vocational High School of Health Services, Ataturk University, 25240 Erzurum
  • Esra Laloglu Departments of Medical Biochemistry of Faculty of Medicine, Ataturk University, Erzurum
  • Kubra Koc Department of Biology, Faculty of Science, Ataturk University, 25240 Erzurum
  • Hayrunnisa Nadaroglu Department of Food Technology, Erzurum Vocational Training School, Ataturk University, Erzurum
  • Fatime Geyikoglu Department of Biology, Faculty of Science, Ataturk University, Erzurum

Keywords:

Morus nigra, hepatic injury, antioxidant enzymes, caspase-3, 8-hydroxydeoxyguanosine

Abstract

In this study, the effect of Morus nigra (M. nigra) on carbon tetrachloride (CCI4)-induced hepatic injury in the rat was investigated. A hepatotoxic rat model was developed by the injection of CCI4 dissolved in soybean oil (1 mL/kg/twice a week, intraperitoneal (i.p.) injection). Following the formation of hepatic injury, extracts of M. nigra at doses ranging from 150 to 300 mg/kg were administered to rats by i.p. injection for eight weeks. At the end of administration, rat livers were excised by dissection. The activities of liver enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT) were detected in the serum, and the activities of antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GPx), were established in the liver. Histological changes and immunohistochemical localization of caspase-3 and 8-oxo-2'-deoxyguanosine (8-OhdG) were performed by hematoxylin-eosin (H&E) staining of liver sections and caspase-3 and 8-OhdG immunohistochemical staining. The results showed that the M. nigra extract prevented protein oxidation generated by CCI4. The extracts demonstrated the ability to modulate the activity of SOD and GPx, and also prevented the CCI4-induced increase in AST and GGT levels. These results indicate that M. nigra extracts provided significant protection against CCl4-induced hepatic injury and might also present a novel approach for the treatment of some liver diseases.

https://doi.org/10.2298/ABS171009055D

Received: October 9, 2017; Revised: December 6, 2017; Accepted: December 12, 2017; Published online: December 22, 2017

How to cite this article: Deniz GY, Laloglu E, Koc K, Nadaroglu H, Geyikoglu F. The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage. Arch Biol Sci. 2018;70(2):371-8.

 

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References

Arbab AH, Parvez MK, Al-Dosari MS, Al-Rehaily AJ, Ibrahim KE, Alam P, Alsaid MS, Rafatullah S. Therapeutic efficacy of ethanolic extract of Aerva javanica aerial parts in the amelioration of CCl4-induced hepatotoxicity and oxidative damage in rats. Food Nutr Res. 2016;60:30864-74.

Ekor M, Odewabi AO, Kale OE, Oritogun KS, Adesanoye OA, Bamidele TO. Pharmacologic inhibition of the renin-angiotensin system did not attenuate hepatic toxicity induced by carbon tetrachloride in rats. Hum Exp Toxicol. 2011;30:1840-8.

LaBrecque DR. World Gastroenterology Organisation global guidelines: Nonalcoholic fatty liver disease and nonalcoholic steatohepatitis. J Clin Gastroenterol. 2014;48: 467-73.

Lam P, Cheung F, Tan HY, Wang N, Yuen MF, Feng Y. Hepatoprotective Effects of Chinese Medicinal Herbs: A Focus on Anti-Inflammatory and Anti-Oxidative Activities. Int J Mol Sci. 2016;17:465.

Chen S, Chen Y, Chen B, Cai YJ, Zou ZL, Wang JG, Lin Z, Wang XD, Fu LY, Hu YR, Chen YP, Chen DZ. Plumbagin Ameliorates CCl4 -Induced Hepatic Fibrosis in Rats via the Epidermal Growth Factor Receptor Signaling Pathway. eCAM. 2015;2015:645727-39.

Jaeschke H, Williams CD, McGill MR, Xie Y, Ramachandran A. Models of drug-induced liver injury for evaluation of phytotherapeutics and other natural products. Food Chem Toxicol. 2013;55:279-89.

Xu S, Touyz RM. Reactive oxygen species and vascular remodelling in hypertension: Still alive. Can J Cardiol. 2006;22:947-51.

Salcedo EM, Sendra E, Barrachina AA. Martínez JJ, Hernández F. Fatty acids composition of Spanish black (Morus nigra L.) and white (Morus alba L.) mulberries. Food Chem. 2016;190:566-71.

Jiang Y, Nie WJ. Chemical properties in fruits of mulberry species from the Xinjiang province of China. Food Chem. 2015;174:460-6.

Gundogdu M, Muradoglu F, Sensoy RIG, Yilmaz H. Determination of fruit chemical properties of Morus nigra L., Morus alba L. and Morus rubra L. by HPLC. Sci Hortic. 2011;132:37-41.

Ross JA, Kasum CM. Dietary flavonoids: bioavailability, metabolic effects, and safety. Annu Rev Nutr. 2002;22:19-34.

Feng RZ, Wang Q, Tong WZ, Xiong J, Wei Q, Zhou WH, Yin ZQ, Yin XY, Wang LY, Chen YQ, Lai YH, Huang HY, Luo QL, Wang L, Jia RY, Song X, Zou YF, Li LX. Extraction and antioxidant activity of flavonoids of Morus nigra. Int J Clinical Exp Med. 2015;8:22328-36.

Volpato GT, Calderon IM, Sinzato S, Campos KE, Rudge MV, Damasceno DC. Effect of Morus nigra aqueous extract treatment on the maternal-fetal outcome, oxidative stress status and lipid profile of streptozotocin-induced diabetic rats. J Ethnopharmacol. 2011;138:691-6.

Akhlaq A, Mehmood MH, Rehman A, Ashraf Z, Syed S, Bawany SA, Gilani AH, Ilyas M, Siddiqui BS. The Prokinetic, Laxative, and Antidiarrheal Effects of Morus nigra: Possible Muscarinic, Ca2+ Channel Blocking, and Antimuscarinic Mechanisms. Phytother Res. 2016;30:1362-76.

Hammer GD, McPhee SJ, editors. Pathophysiology of disease: An introduction to clinical medicine. 6th ed. New York: McGraw-Hill Medical; 2014. p. 14.

Basu S. Carbon tetrachloride-induced lipid peroxidation: eicosanoid formation and their regulation by antioxidant nutrients. Toxicology. 2003;189:113-27.

GBD 2013 Mortality and Causes of Death Collaborators. Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet. 2015;385:117-71.

Chen H, Pu J, Liu D, Yu W, Shao Y, Yang G, Xiang Z, He N. Anti-Inflammatory and Antinociceptive Properties of Flavonoids from the Fruits of Black Mulberry (Morus nigra L.). PLoS One. 2016;11:e0153080.

Lin HM, Tseng HC, Wang CJ, Lin JJ, Lo CW, Chou FP. Hepatoprotective effects of Solanum nigrum Linn extract against CCl4-induced oxidative damage in rats. Chem-Biol Interact. 2008;171:283-93.

Parola M, Robino G. Oxidative stress-related molecules and liver fibrosis. J Hepatology. 2001;35:297-306.

Pawlowska AM, Oleszek W, Braca A. Quali-quantitative Analyses of Flavonoids of Morus nigra L. and Morus alba L. (Moraceae) Fruits. J Agric Food Chem. 2008;56:3377-80.

Aghababaee SK, Vafa M, Shidfar F, Tahavorgar A, Gohari M, Katebi D, Mohammadi V. Effects of blackberry (Morus nigra L.) consumption on serum concentration of lipoproteins, apo A-I, apo B, and high-sensitivity-C-reactive protein and blood pressure in dyslipidemic patients. J Res Med Sci. 2015;20:684-91.

Mallhi TH, Qadir MI, Khan YH, Ali M. Hepatoprotective activity of aqueous methanolic extract of Morus nigra against paracetamol-induced hepatotoxicity in mice. Bangl J Pharmacol. 2014;9:60-6.

Tag HM. Hepatoprotective effect of mulberry (Morus nigra) leaves extract against methotrexate induced hepatotoxicity in male albino rat. BMC Complem Altern M. 2015;15:252-61.

Hassanalilou T, Payahoo L, Shahabi P, Abbasi MM, Jafar-abadi MA, Bishak YK, Khordadmehr M, Esnaashari S, Barzegar A. The protective effects of Morus nigra L. leaves on the kidney function tests and histological structures in streptozotocin-induced diabetic rats. Biomed Res. 2017;28(In press).

Araujo CM, de Pádua Lúcio K, Silva ME, Isoldi MC, de Souza GHB, Brandão GC, Schulz R, Costa DC. Morus nigra leaf extract improves glycemic response and redox profile in the liver of diabetic rats. Food Funct. 2015;6:3490-9.

Basavaraj K, Vasu Devaraju P, Rao K. Studies on serum 8-hydroxy guanosine (8-OHdG) as reliable biomarker for psoriasis. J Eur Acad Dermatol. 2013;27:655-7.

Montuschi P, Barnes P, Roberts LJ. Insights into oxidative stress: the isoprostanes. Curr Med Chem. 2007;14:703-17.

Chu X, Wang H, Jiang YM, Zhang YY, Bao YF, Zhang X, Zhang JP, Guo H, Yang F, Luan YC, Dong YS. Ameliorative effects of tannic acid on carbon tetrachloride-induced liver fibrosis in vivo and in vitro. J Pharmacol Sci. 2015;130:15-23.

Sansoè G, Aragno M, Mastrocola R, Mengozzi G, Novo E, Parola M. Role of Chymase in the Development of Liver Cirrhosis and Its Complications: Experimental and Human Data. PLoS One. 2016;11:e0162644.

Pober JS, Min W, Bradley JR Mechanisms of endothelial dysfunction, injury, and death. Annu Rev Path. 2009;4:71-95.

Gul F, Muderris T, Yalciner G, Sevil E, Bercin S, Ergin M, Babademez MA, Kiris M. A comprehensive study of oxidative stress in sudden hearing loss. Eur Arch Oto-Rhino-l. 2016;273:1-8.

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Published

2018-05-30

How to Cite

1.
Deniz GY, Laloglu E, Koc K, Nadaroglu H, Geyikoglu F. The effect of black mulberry (Morus nigra) extract on carbon tetrachloride-induced liver damage. Arch Biol Sci [Internet]. 2018May30 [cited 2022Sep.29];70(2):371-8. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2244

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