Impact of moderate heat stress on the biochemical and physiological responses of the invasive waterweed Elodea canadensis (Michx. 1803)
Keywords:Elodea canadensis, antioxidants, moderate heat stress, malondialdehyde, electrolyte leakage
- The influence of climate change on biochemical and physiological changes in freshwater macrophytes has been widely studied. Most studies have focused on the responses to stressors such as hypoxia, salinity and heavy metals. Less attention has been paid to heat-induced oxidative stress and the activity of the antioxidant system, despite the fact that elevated water temperature can severely affect them.
- This study provides new findings on the changes in oxidative processes, lipid peroxidation, membrane stability and antioxidant defence in aquatic macrophytes under high temperature treatment.
- The presented data expand on existing knowledge.
Abstract: Global warming can negatively affect freshwater macrophytes. However, the degree to which freshwater plants can survive after long-term or short-term warming and the underlying molecular mechanisms are not fully understood. The aim of our study was to analyze the responses of an invasive plant to moderate heat stress (HS). Biochemical and physiological stress responses to experimental warming (30±1.0°C/25±1.0°C, day/night) were assessed in the invasive waterweed Elodea canadensis. The effect of the moderate HS on the macrophyte was evaluated through changes in the total protein content, catalase activity, lipid peroxidation, cellular membrane permeability by electrolyte leakage and the concentrations of carotenoids and photosynthetic pigments. Catalase activity and carotenoid concentrations increased significantly (p<0.01) in comparison to the control. A significant increase (p<0.05) in malondialdehyde concentration was observed. However, at the same time there was a persistent low level of electrolyte leakage in heat-treated plants as compared to the control. The results demonstrated that moderate HS improved membrane stability and increased the concentration of photosynthetic pigments and antioxidant activity in E. canadensis shoots. Moderate alterations in temperature may favorably affect the physiology and growth of the invasive macrophyte E. canadensis. It is reasonable to expect that warming could lead to a gradual change in E. canadensis distribution and to changes in composition of freshwater ecosystems.
Received: January 19, 2018; Revised: April 10, 2018; Accepted: April 10, 2018; Published online: April 18, 2018
How to cite this article: Savicka M, Petjukevičs A, Batjuka A, Škute N. Impact of moderate heat stress on the biochemical and physiological responses of the invasive waterweed Elodea canadensis (Michx. 1803). Arch Biol Sci. 2018;70(3):…
Zhang X, Odgaard R, Olesen B, Lauridsen TL, Liboriussen L, Søndergaard M, Liu Z, Jeppesen E. Warming shows differential effects on late-season growth and competitive capacity of Elodea canadensis and Potamogeton crispus in shallow lakes. Inland Waters. 2015;5:421-32.
Hossain K, Yadav S, Quaik S, Pant G, Maruthi AY, Ismail N. Vulnerabilities of macrophytes distribution due to climate change. Theor Appl Climatol. 2017;129:1123-32.
Silveira MJ, Thiébaut G. Impact of climate warming on plant growth varied according to the season. Limnologica. 2017;65:4-9.
Wahid A, Gelani S, Ashraf M, Foolad MR. Heat tolerance in plants: an overview. Environ Exp Bot. 2007;61:199-223.
Rybicki NB, Carter V. Light and temperature effects on the growth of wild celery and hydrilla. J Aquat Plant Manag. 2002;40:92-9.
Hughes L. Biological consequences of global warming: Is the signal already apparent. Trends Ecol Evol. 2000;15:56-61.
Jacobs AFG, Jetten TH, Lucassen DC, Heusinkveld BJ, Nieveen JP. Diurnal temperature fluctuations in a natural shallow water body. Agric For Meteorol. 1997;88(1-4):269-77.
Jacobs AFG, Heusinkveld BG, Kraai A, Paaijmans, KP. Diurnal temperature fluctuations in an artificial small shallow water body. Int J Biometeorol. 2008;52(4), 271-80.
Mittler R, Finka A, Goloubinoff P. How do plants feel the heat? Trends Biochem Sci. 2012;37(3):118-25.
Sairam R, Tyagi A. Physiology and molecular biology of salinity stress tolerance in plants. Curr Sci. 2004;86(3):407-21.
Kasote DM, Katyare SS, Hegde MV, Bae H. Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci. 2015;11(8):982-91.
Gururani MA, Venkatesh J, Tran LSP. Regulation of photosynthesis during abiotic stress-induced photoinhibition. Mol Plant. 2015;8(9):1304-20.
Sharma P, Jha AB, Dubey RS, Pessarakli M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot. 2012;2012:217037.
Anderson JA, Padhye SR. Protein aggregation, radical scavenging capacity, and stability of hydrogen peroxide defence systems in heatstressed vinca and sweet pea leaves. J Am Soc Hortic Sci. 2004;129:54-9.
Savicka M, Škute N. Some morphological, physiological and biochemical characteristics of wheat seedling Triticum aestivum L. organs after high-temperature treatment. Ekologija. 2012;58(1):9-21.
Kipp E, Boyle M. The effects of heat stress on reactive oxygen species production and chlorophyll concentration in Arabidopsis thaliana. Res Plant Sci. 2013;1:20-3.
Nayek S, Gupta S, Saha R. Effects of metal stress on biochemical response of some aquatic macrophytes growing along an industrial waste discharge channel. J Plant Interact. 2010;5(2):91-9.
Netten JJC, Van der Heide T, Smolders AJP. Interactive effects of pH, temperature and light during ammonia toxicity events in Elodea Canadensis. Chem Ecol. 2013;29(5):448-58.
Petjukevičs A, Batjuka A, Škute N. The impact of different levels of sodium chloride on the quantitative changes of chlorophyll and carotenoids in chloroplasts of Elodea canadensis (Michx. 1803). Biologija. 2015;61(1):34-41.
Parveen M, Asaeda T, Rashid MH. Biochemical adaptations of four submerged macrophytes under combined exposure to hypoxia and hydrogen sulphide. PLoS ONE. 2017;12(8):e0182691
Gaya KS, Mathew L, Ramesh Babu MG. An assessment of heavy metal accumulation capacity of five aquatic macrophytes and biochemical response. Int J Adv Res. 2017;5(10):839-47.
Jeppesen E, Søndergaard M, Jensen JP. Climate warming and regime shifts in lake food webs - some comments. Limnol Oceanogr. 2003;48:1346-49.
Grīnberga L, Priede A. Elodea canadensis Michx. in Latvia. Acta Biol Univ Daugavp. 2010;10(1):43-50.
Josefsson M, Andersson B. The environmental consequences of alien species in the Swedish lakes Mälaren, Hjälmaren, Vänern and Vättern. AMBIO. 2002;30(8):514-21.
Pagano AM, Titus JE. Submersed macrophyte growth at low pH: contrasting responses of three species to dissolved inorganic carbon enrichment and sediment type. Aquat Bot. 2004;79:65-74.
Madsen TV, Brix H. Growth, phytosynthesis and acclimation by two submerged macrophytes in relation to temperature. Oecologia. 1997;110: 320-7.
Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121-26.
Guo Z, Ou W, Lu S, Zhong Q. Differential responses of anti-oxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiol Biochem. 2006;44:828-36.
Ali MB, Hahn EJ, Paek KY. Effects of light intensities on antioxidant enzymes and malondialdehyde content during short-term acclimatization on micro-propagated Phalaenopsis plantlet. Environ Exp Bot. 2005;54(2):109-20.
Lichtenthaler HK. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol. 1987;148:350-82.
Pilon J, Santamaría L. Seasonal acclimation in the photosynthetic and respiratory temperature responses of three submerged freshwater macrophyte species. New Phytologist. 2001;151(3):659-70.
Hasanuzzaman M, Nahar K, Alam MM, Roychowdhury R, Fujita M. Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. Int J Mol Sci. 2013;14(5):9643-84.
Siddiqui MH, Al-Whaibi MH, Sakran AM, Ali HM, Basalah MO, Faisal M, Alatar A, Al-Amri AA. Calcium-induced amelioration of boron toxicity in radish. J Plant Growth Regul. 2013;32:61-71.
Han Y, Fan S, Zhang Q, Wang Y. Effect of heat stress on the MDA, proline and soluble sugar content in leaf lettuce seedlings. Agric Sci. 2013;4(5b):112-15.
Busaidi AKTS, Farag KM. The use of electrolyte leakage procedure in assessing heat and salt tolerance of Ruzaiz date palm (Phoenix dactylifera L.) cultivar regenerated by tissue culture and offshoots and treatments to alleviate the stressful injury. J Hort For. 2015;7(4):104-11.
Toscano S, Farieri E, Ferrante A, Romano D. Physiological and biochemical responses in two ornamental shrubs to drought stress. Front Plant Sci. 2016;7:645.
Schweiggert RM, Ziegler JU, Metwali EMR, Almaghrabi OA, Kadasa NM, Carle R. Carotenoids in mature green and ripe red fruits of tomato (Solanum lycopersicum L.) grown under different levels of irrigation. Arch Biol Sci. 2017;69(2):305-14.
Tripathy BC, Oelmüller R. Reactive oxygen species generation and signalling in plants. Plant Signal Behav. 2012;7(12):1621-33.
Zakar T, Laczko-Dobos H, Toth TN, Gombos Z. Carotenoids assist in cyanobacterial photosystem II assembly and function. Front Plant Sci. 2016;7:295.
Chalanika De Silva HC, Asaeda T. Effects of heat stress on growth, photosynthetic pigments, oxidative damage and competitive capacity of three submerged macrophytes. J Plant Interact. 2017;12(1):228-36.
Maleva M, Borisova G, Chukina N, Nekrasova G, Prasad MN. Influence of exogenous urea on photosynthetic pigments, (14)CO 2 uptake, and urease activity in Elodea densa - environmental implications. Environ Sci Pollut Res Int. 2013;20(9):6172-7.
Demars BOL, Trémolières M. Aquatic macrophytes as bioindicators of carbon dioxide in groundwater fed rivers. Sci Total Environ. 2009;407(16):4752-63.
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