Effects of increased proliferation of human adipose tissue-derived mesenchymal stem cells by sphingosylphosphorylcholine on the survival of cryopreserved fat grafts
Keywords:human adipose-derived stem cell (hADSC), sphingosylphosphorylcholine (SPC), cryopreservation, fat, angiogenesis
- The use of cryopreserved adipose tissue for soft tissue augmentation is common, but the unpredictability of fat graft viability remains a limitation.
- We demonstrated the effects of human adipose-derived stem cells (hADSC) plus sphingosylphosphorylcholine (SPC) on the survival of cryopreserved fat grafts in BALB/c male nude mice. The hADSC+SPC group showed higher survival rate in terms of weight and volume than the control or hADSC group. The hADSC+SPC treatment significantly increased the expression of angiogenic factors.
- These results point to the potential clinical benefit of hADSC+SPC.
Abstract: The use of cryopreserved adipose tissue for soft-tissue augmentation is common, but the unpredictability of fat graft viability remains a limitation. Human adipose-derived stem cells (hADSC) have been introduced to enhance viability and improve the survival of transplanted fat tissue. Sphingosylphosphorylcholine (SPC) is a bioactive lipid molecule involved in various cellular responses. SPC stimulates the proliferation of various cell types such as hADSC. We demonstrated the effects of hADSC and SPC on the survival of cryopreserved fat grafts in nude mice. The cryopreserved fat grafts were treated with hADSC or hADSC+SPC, and a normal saline (control) mixture in BALB/c male nude mice. We examined the weight and volume of the mice in each group (n=11) at 8 weeks after transplantation to evaluate the survival of the fat tissue. The hADSC group showed increased weight and volume compared with the control group. The hADSC+SPC group showed a higher survival rate in terms of weight and volume than the control or hADSC group. In addition, the hADSC+SPC treatment significantly increased the expression of angiogenic factors. These results suggest the potential clinical utility of hADSC+SPC.
Received: February 20, 2018; Revised: April 12, 2018; Accepted: April 13, 2018; Published online: April 17, 2018
How to cite this article: Bae YC, Song JS, Nam KW, Kim JH, Nam SB. Effects of increased proliferation of human adipose tissue-derived mesenchymal stem cells by sphingosylphosphorylcholine on the survival of cryopreserved fat grafts. Arch Biol Sci. 2018;70(3):…
Locke MB, de Chalain TM. Current practice in autologous fat transplantation: suggested clinical guidelines based on a review of recent literature. Ann Plast Surg. 2008;60(1):98-102.
Horl HW, Feller AM, Biemer E. Technique for liposuction fat reimplantation and long-term volume evaluation by magnetic resonance imaging. Ann Plast Surg. 1991;26(3):248-58.
Pu LL, Coleman SR, Cui X, Ferguson RE Jr, Vasconez HC. Cryopreservation of autologous fat grafts harvested with the Coleman technique. Ann Plast Surg. 2010;64(3):333-7.
Yoshimura K, Sato K, Aoi N, Kurita M, Hirohi T, Harii K. Cell-assisted lipotransfer for cosmetic breast augmentation: supportive use of adipose-derived stem/stromal cells. Aesthetic Plast Surg. 2008;32(1):48-57.
Yoshimura K, Sato K, Aoi N, Kurita M, Inoue K, Suga H, Eto H, Kato H, Hirohi T, Harii K. Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg. 2008;34(9):1178-85.
Shoshani O, Shupak A, Ullmann Y, Ramon Y, Gilhar A, Kehat I, Peled IJ. The effect of hyperbaric oxygenation on the viability of human fat injected into nude mice. Plast Reconstr Surg. 2000;106(6):1390-8.
Yi C, Pan Y, Zhen Y, Zhang L, Zhang X, Shu M, Han Y, Guo S. Enhancement of viability of fat grafts in nude mice by endothelial progenitor cells. Dermatol Surg. 2006;32(12):1437-43.
Bae YC, Song JS, Bae SH, Kim JH. Effects of human adipose-derived stem cells and stromal vascular fraction on cryopreserved fat transfer. Dermatol Surg. 2015;41(5):605-14.
Meyer zu Heringdorf D, Himmel HM, Jakobs KH. Sphingosylphosphorylcholine-biological functions and mechanisms of action. Biochim Biophys Acta. 2002;1582(1-3):178-89.
Desai NN, Spiegel S. Sphingosylphosphorylcholine is a remarkably potent mitogen for a variety of cell lines. Biochem Biophys Res Commun. 1991;181(1):361-6.
Chin TY, Chueh SH. Sphingosylphosphorylcholine stimulates mitogen-activated protein kinase via a Ca2+-dependent pathway. Am J Physiol. 1998;275(5 Pt 1):C1255-63.
Sun L, Xu L, Henry FA, Spiegel S, Nielsen TB. A new wound healing agent--sphingosylphosphorylcholine. J Invest Dermatol. 1996;106(2):232-7.
Wakita H, Matsushita K, Nishimura K, Tokura Y, Furukawa F, Takigawa M. Sphingosylphosphorylcholine stimulates proliferation and upregulates cell surface-associated plasminogen activator activity in cultured human keratinocytes. J Invest Dermatol. 1998;110(3):253-8.
Jeon ES, Song HY, Kim MR, Moon HJ, Bae YC, Jung JS, Kim JH. Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK. J Lipid Res. 2006;47(3):653-64.
Kim YJ, Kim HK, Cho HH, Bae YC, Suh KT, Jung JS. Direct comparison of human mesenchymal stem cells derived from adipose tissues and bone marrow in mediating neovascularization in response to vascular ischemia. Cell Physiol Biochm. 2007;20(6):867-76.
Ferguson RE, Cui X, Fink BF, Vasconez HC, Pu LL. The viability of autologous fat grafts harvested with the LipiVage system: a comparative study. Ann Plast Surg. 2008;60(5):594-7.
Pietrzak WS, Eppley BL. Platelet rich plasma: biology and new technology. J Craniofac Surg. 2005;16(6):1043-54.
Ayhan M, Senen D, Adanali G, Gorgu M, Erdogan B, Albayrak B. Use of beta blockers for increasing survival of free fat grafts. Aesthetic Plast Surg. 2001;25(5):338-42.
Nixon GF, Mathieson FA, Hunter I. The multi-functional role of sphingosylphosphorylcholine. Prog Lipid Res. 2008;47(1):62-75.
Xu Y. Sphingosylphosphorylcholine and lysophosphatidylcholine: G protein-coupled receptors and receptor-mediated signal transduction. Biochim Biophys Acta. 2002;1582(1-3):81-8.
Seufferlein T, Rozengurt E. Sphingosylphosphorylcholine activation of mitogen-activated protein kinase in Swiss 3T3 cells requires protein kinase C and a pertussis toxin-sensitive G protein. J Biol Chem. 1995;270(41):24334-42.
Bae YC, Choi CW, Nam KW, Song JS, Lee JW. Effects of sphingosylphosphorylcholine on cryopreserved fat tissue graft survival. Mol Med Rep. 2016;14(4):3719-24.
Jeon ES, Lee MJ, Sung SM, Kim JH. Sphingosylphosphorylcholine induces apoptosis of endothelial cells through reactive oxygen species-mediated activation of ERK. J Cell Biochem. 2007;100(6):1536-47.