Overexpression of the mulberry latex gene MaMLX-Q1 enhances defense against <i>Plutella xylostella</i> in <i>Arabidopsis thaliana</i>

Authors

  • Yan Liu Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou, 310021
  • Dongfeng Ji Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou, 310021
  • Jine Chen Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou, 310021
  • Tianbao Lin Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou, 310021
  • Jia Wei Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou, 310021
  • Yan Zhu Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou, 310021
  • Zhiqiang Lv Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou, 310021

Keywords:

chitinase, Plutella xylostella, transgenic Arabidopsis thaliana, latex, mulberry

Abstract

Purified mulberry latex chitinase (MLX) has a role in defense against some lepidopteran insects. In this study, a full length chitinase gene, MaMLX-Q1, of 1405 bp with a 1140 bp open reading frame, was obtained from mulberry leaves by the degenerate primers and rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR) procedure. The gene encoded a mature protein with the predicted molecular mass of 39.38 kDa and an isoelectric point (pI) of 6.43; it contained two chitin-binding domains and a hydrolase family 19 chitinase domain. Sequence alignment and phylogenetic analysis grouped it in the class I chitinase protein group. Heterogeneous expression of this MaMLX-Q1 in Arabidopsis showed non-visible alterations in growth phenotype, except for the higher transcriptional expression of MaMLX-Q1 when compared to that of wild-type Arabidopsis. This ectopic MaMLX-Q1 exhibited toxicity to the growth and development of Plutella xylostella larvae, causing significantly lower weight gain and higher mortality. These results indicate an application of MaMLX-Q1 as an insecticide for plant protection.

https://doi.org/10.2298/ABS160315097L

Received: March 15, 2016; Revised: April 24, 2016; Accepted: May 4, 2016; Published online: October 17, 2016

How to cite this article: Liu Y, Ji D, Chen J, Lin T, Wei J, Zhu Y, Lv Z. Overexpression of the mulberry latex gene MaMLX-Q1 enhances defense against Plutella xylostella in Arabidopsis thaliana. Arch Biol Sci. 2017;69(2):269-76.

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References

Konno K, Ono H, Nakamura M, Tateishi K, Hirayama C, Tamura Y, Hattori M, Koyama A, Kohno K. Mulberry latex rich in antidiabetic sugar-mimic alkaloids forces dieting on caterpillars. Proc Natl Acad Sci U S A. 2006;103(5):1337-41.

Pickard WF. Laticifers and secretory ducts: two other tube systems in plants. New Phytol. 2008;177(4):877-88.

Lewinsohn TM. The geographical distribution of plant latex. Chemoecology. 1991;2(1);64-8.

Agrawal AA, Konno K. Latex: a model for understanding mechanisms, ecology, and evolution of plant defense against herbivory. Annu Rev Ecol Evol Syst. 2009;40(1):311-31.

Dussourd DE, Eisner T. Vein-cutting behavior: insect counterploy to the latex defense of plants. Science. 1987;237(4817):898-90.

Sethi A, McAuslane HJ, Rathinasabapathi B, Nuessly GS, Nagata RT. Enzyme induction as a possible mechanism for latex-mediated insect resistance in romaine lettuce. J Chem Ecol. 2009;35(2):190-200.

Konno K. Plant latex and other exudates as plant defense systems: roles of various defense chemicals and proteins contained therein Phytochemistry. 2011;72(13):1510-30.

Inui H, Sawada M, Goto J, Yamazaki K, Kodama N, Tsuruta H, Eun H. A major latex-like protein is a key factor in crop contamination by persistent organic pollutants. Plant Physiol. 2013;161(4):2128-35

Kitajima S, Kamei K, Taketani S, Yamaguchi M, Kawai F, Komatsu A, Inukai Y. Two chitinase-like proteins abundantly accumulated in latex of mulberry show insecticidal activity. BMC Biochem. 2010;11:6.

Mithöfer A, Boland W. Plant defense against herbivores: chemical aspects. Annu Rev Plant Biol. 2012;63:431-50.

Wasano N, Konno K, Nakamura M, Hirayama C, Hattori M, Tateishi K. A unique latex protein, MLX56, defends mulberry trees from insects. Phytochemistry. 2009;70(7):880-8

Clough SJ, Bent AF. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1998;16(6):735-43.

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔC(T) Method. Methods. 2001;25(4):402-8.

Wang X, He N, Zeng Q, Xiang Z. Identification and expression analyses of chitinase genes in mulberry (Morus L.) plants. Plant Omics Journa. 2015;8(2):183-90.

Kitajima S, Taira T, Oda K, Yamato KT, Inukai Y, Hori Y. Comparative study of gene expression and major proteins’ function of laticifers in lignified and unlignified organs of mulberry. Planta.2012; 235 (3):589-601.

Carroll CR, Hoffman CA. Chemical feeding deterrent mobilized in response to insect herbivory and counteradaptation by Epilachna tredecimnotata. Science. 1980;209(4454):414-6.

Dussourd DE, Denno RF. Deactivation of Plant Defense: Correspondence Between Insect Behavior and Secretory Canal Architecture. Ecology.1991;72(4):1383-96.

Trapp S, Croteau R. Defensive resin biosynthesis in conifers. Annu Rev Plant Physiol Plant Mol Biol. 2001;52:689-724.

Daimon T, Taguchi T, Meng Y, Katsuma S, Mita K, Shimada T. Beta-fructofuranosidase genes of the silkworm, Bombyx mori: insights into enzymatic adaptation of B. mori to toxic alkaloids in mulberry latex. J Biol Chem. 2008;283(22):15271-9.

He N, Zhang C, Qi X, Zhao S, Tao Y, Yang G, Lee TH, Wang X, Cai Q, Li D, Lu M, Liao S, Luo G, He R, Tan X, Xu Y, Li T, Zhao A, Jia L, Fu Q, Zeng Q, Gao C, Ma B, Liang J, Wang X, Shang J, Song P, Wu H, Fan L, Wang Q, Shuai Q, Zhu J, Wei C, Zhu-Salzman K, Jin D, Wang J, Liu T, Yu M, Tang C, Wang Z, Dai F, Chen J, Liu Y, Zhao S, Lin T, Zhang S, Wang J, Wang J, Yang H, Yang G, Wang J, Paterson AH, Xia Q, Ji D, Xiang Z. Draft genome sequence of the mulberry tree Morus notabilis. Nat Commun; 2013;4:2445.

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Published

2017-05-25

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1.
Liu Y, Ji D, Chen J, Lin T, Wei J, Zhu Y, Lv Z. Overexpression of the mulberry latex gene MaMLX-Q1 enhances defense against &lt;i&gt;Plutella xylostella&lt;/i&gt; in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;. Arch Biol Sci [Internet]. 2017May25 [cited 2022Aug.9];69(2):269-76. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/338

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