Resveratrol blocks atherosclerosis development by inhibiting IL-1β in macrophages induced by cholesterol


  • Yilin Xie School of Life Science and Biotechnology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200240
  • Zhaoxia Wang School of Life Science and Biotechnology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200240
  • Haiyun Lin School of Life Science and Biotechnology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200240
  • Yajun Pan School of Life Science and Biotechnology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200240
  • Lianyun Wang School of Life Science and Biotechnology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200240
  • Zhiqiang Yan Central Laboratory, Shanghai Jiao Tong University Affiliated Sixth Peoples’ Hospital South Campus Shanghai, Shanghai 201400
  • Zhongdong Qiao School of Life Science and Biotechnology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200240
  • Zhihua Han Department of Cardiology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011


resveratrol, IL-1β, atherosclerosis, cholesterol, HUVEC


Paper description:

  • Resveratrol inhibits the cholesterol-induced production of interleukin-1 beta (IL-1β) in macrophages and reduces adhesion of monocyte to the endothelium. Resveratrol could also exert its anti-inflammatory roles by stimulating reversed cholesterol transport (RCT) in macrophages.
  • Resveratrol inhibited monocyte recruitment partly through the inhibition of IL-1β expression in macrophages. We hypothesize that resveratrol may exert its anti-inflammatory effects by stimulating RCT and by inhibiting the deposition of cholesterol in macrophages.
  • These findings enhance the potential value of resveratrol in the prevention of atherosclerosis.

Abstract: Resveratrol is a polyphenolic compound that exhibits antiinflammatory and cardioprotective properties. In this study we investigated the protective role of resveratrol on the inflammatory activation of macrophages during pathogenesis of atherosclerosis. Macrophage Ana-1 cells were stimulated by cholesterol and resveratrol, and the cell culture supernatant was collected to treat human umbilical vein endothelial cells (HUVECs). The release of IL-1β into the Ana-1 cell supernatant was quantified by ELISA. Expression of the adhesion molecule ICAM-1 and E-selectin in HUVECs were examined by Western-blotting. Additionally, the adhesion of monocytes in HUVECs under different conditions was tested by cell adhesion analyses. The results indicated that the high cholesterol treatment increased the expression level of IL-1β, while pretreatment with resveratrol inhibited this induction of IL-1β in Ana-1 cells. Resveratrol inhibited the adhesion of monocytes to the endothelium at least partly through inhibition of IL-1β expression in macrophages. Moreover, the expression level of caveolin-1 significantly increased after the pretreatment with resveratrol, indicating that resveratrol enhances reverse cholesterol transport (RCT) in macrophages. Our study indicated that resveratrol has significant antiinflammatory effects and can be considered as a candidate molecule to prevent atherosclerosis.

Received: November 29, 2018; Revised: April 2, 2019; Accepted: April 3, 2019; Published online: July 10, 2019

How to cite this article: Xiea Y, Wanga Z, Lin H, Pan Y, Wang L, Yan Z, Qiao Z, Han Z. Resveratrol blocks atherosclerosis development by inhibiting IL-1β in macrophages induced by cholesterol. Arch Biol Sci. 2019;71(3):551-9.


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How to Cite

Xie Y, Wang Z, Lin H, Pan Y, Wang L, Yan Z, Qiao Z, Han Z. Resveratrol blocks atherosclerosis development by inhibiting IL-1β in macrophages induced by cholesterol. Arch Biol Sci [Internet]. 2019Oct.23 [cited 2022Aug.9];71(3):551-9. Available from: