Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra

Authors

  • Zexiong Chen College of Forestry and Life Science, Chongqing University of Arts and Sciences, Chongqing 402160
  • Juan Lou College of Forestry and Life Science, Chongqing University of Arts and Sciences, Chongqing 402160

Keywords:

TPS, light wavelength treatments, vegetative growth, floral transition, tomato

Abstract

Light is the source of energy for plants. Light wavelengths, densities and irradiation periods act as signals directing morphological and physiological characteristics during plant growth and development. To evaluate the effects of light wavelengths on tomato growth and development, Solanum lycopersicum (cv. micro-Tom) seedlings were exposed to different light-quality environments, including white light and red light supplemented with blue light (at ratios of 3:1 and 8;1, respectively). Tomatoes grown under red light supplemented with blue light displayed significantly shorter stem length, a higher number of flower buds and rate of fruit set, but an extremely late flowering compared to white-light-grown plants. To illustrate the mechanism underlying the inhibition of stem growth and floral transition mediated by red/blue light, 10 trehalose-6-phosphate synthase (TPS) genes were identified in tomato, and bioinformatics analysis was performed. qRT-PCR analysis showed that SlTPSs were expressed widely throughout plant development and SlTPS1 was expressed at extremely high levels in stems and buds. Further analysis of several flowering-associated genes and microRNAs showed that the expressions of SlTPS1, SlFT and miR172 were significantly downregulated in tomato grown under red and blue light compared with those grown under white light, whereas miR156 transcript levels were increased. A regulatory model underlying vegetative growth and floral transition regulated by light qualities is presented. Our data provide evidence that light quality strongly affects plant growth and phase transition, most likely via the TPS1-T6P signaling pathway.

DOI: 10.2298/ABS160325082C

Received: March 25, 2016; Revised: June 13, 2016; Accepted: July 26, 2016; Published online: September 21, 2016

How to cite this article: Chen Z, Lou J. Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra. Arch Biol Sci. 2017;69(1):93-101.

 

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References

Schaffer R, Landgraf J, Accerbi M, Simon V, Larson M, Wisman E. Microarray analysis of diurnal and circadian-regulated genes in Arabidopsis. Plant Cell. 2001;13(1):113-23.

Tepperman JM, Hudson ME, Khanna R, Zhu T, Chang SH, Wang X, Quail PH. Expression profiling of phyB mutant demonstrates substantial contribution of other phytochromes to red-light-regulated gene expression during seedling de-etiolation. Plant J. 2004;38(5):725-39.

Smith H. Phytochromes and light signal perception by plants − an emerging synthesis. Nature. 2000;407(6804):585-91.

Srikanth A, Schmid M. Regulation of flowering time: all roads lead to Rome. Cell Mol Life Sci. 2011;68(12):2013-37.

Koch KE. Carbohydrate-Modulated Gene Expression in Plants. Annu Rev Plant Physiol Plant Mol Biol. 1996;47:509-40.

Cabib E, Leloir LF. The biosynthesis of trehalose phosphate. J Biol Chem. 1958;231(1):259-75.

Lunn JE, Feil R, Hendriks JH, Gibon Y, Morcuende R, Osuna D, Scheible WR, Carillo P, Hajirezaei MR, Stitt M. Sugar-induced increases in trehalose 6-phosphate are correlated with redox activation of ADP glucose pyrophosphorylase and higher rates of starch synthesis in Arabidopsis thaliana. Biochem J. 2006;397(1):139-48.

Eastmond PJ, van Dijken AJ, Spielman M, Kerr A, Tissier AF, Dickinson HG, Jones JD, Smeekens SC, Graham IA. Trehalose-6-phosphate synthase 1, which catalyses the first step in trehalose synthesis, is essential for Arabidopsis embryo maturation. Plant J. 2002;29(2):225-35.

Gomez LD, Gilday A, Feil R, Lunn JE, Graham IA. AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells. Plant J. 2010;64(1):1-13.

van Dijken AJ, Schluepmann H, Smeekens SC. Arabidopsis trehalose-6-phosphate synthase 1 is essential for normal vegetative growth and transition to flowering. Plant Physiol. 2004;135(2):969-77.

Wahl V, Ponnu J, Schlereth A, Arrivault S, Langenecker T, Franke A, Feil R, Lunn JE, Stitt M, Schmid M. Regulation of flowering by trehalose-6-phosphate signaling in Arabidopsis thaliana. Science. 2013;339(6120):704-7.

Thimijan ,R. W., and R.D. Heins. Photometrics, radiometric and quantum light units of measure: A review of procedures for interconversion. HortScience. 1983;18(6):818-22.

The tomato genome sequence provides insights into fleshy fruit evolution. Nature. 2012;485(7400):635-41.

Xian Z, Huang W, Yang Y, Tang N, Zhang C, Ren M, Li Z. miR168 influences phase transition, leaf epinasty, and fruit development via SlAGO1s in tomato. Journal of experimental botany. 2014;65(22):6655-66.

Hoover WH. The dependence of carbon dioxide assimilation in a higher plant on wavelength of radiation. Smithson Inst Misc. 1933;95:1-13.

McCree KJ. Significance of enhancement for calculations based on the action spectrum for photosynthesis. Plant Physiol. 1972;49(5):704-6.

Efimtsev EI, Boichenko VA, Litvin FF. Action spectra of photosynthesis and liberation of hydrogen in bacteria, algae and higher plants. Dokl Akad Nauk SSSR. 1975;220(5):1238-40.

Hogewoning SW, Wientjes E, Douwstra P, Trouwborst G, van Ieperen W, Croce R, Harbinson J. Photosynthetic quantum yield dynamics: from photosystems to leaves. Plant Cell. 2012;24(5):1921-35.

Brown CS, Schuerger AC, Sager JC. Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. J Am Soc Hortic Sci. 1995;120(5):808-13.

Goins GD, Yorio NC, Sanwo MM, Brown CS. Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with and without supplemental blue lighting. J Exp Bot. 1997;48(312):1407-13.

Yorio NC, Goins GD, Kagie HR, Wheeler RM, Sager JC. Improving spinach, radish, and lettuce growth under red light-emitting diodes (LEDs) with blue light supplementation. HortScience. 2001;36(2):380-3.

Matsuda R, Ohashi-Kaneko K, Fujiwara K, Kurata K. Effects of blue light deficiency on acclimation of light energy partitioning in PSII and CO2 assimilation capacity to high irradiance in spinach leaves. Plant Cell Physiol. 2008;49(4):664-70.

Smith AM, Stitt M. Coordination of carbon supply and plant growth. Plant Cell Environ. 2007;30(9):1126-49.

Schluepmann H, Pellny T, van Dijken A, Smeekens S, Paul M. Trehalose 6-phosphate is indispensable for carbohydrate utilization and growth in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2003;100(11):6849-54.

Kolbe A, Oliver SN, Fernie AR, Stitt M, van Dongen JT, Geigenberger P. Combined transcript and metabolite profiling of Arabidopsis leaves reveals fundamental effects of the thiol-disulfide status on plant metabolism. Plant Physiol. 2006;141(2):412-22.

Leyman B, Van Dijck P, Thevelein JM. An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana. Trends Plant Sci. 2001;6(11):510-3.

Blazquez MA, Santos E, Flores CL, Martinez-Zapater JM, Salinas J, Gancedo C. Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase. Plant J. 1998;13(5):685-9.

Zang B, Li H, Li W, Deng XW, Wang X. Analysis of trehalose-6-phosphate synthase (TPS) gene family suggests the formation of TPS complexes in rice. Plant Mol Biol. 2011;76(6):507-22.

Wang JW, Czech B, Weigel D. miR156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana. Cell. 2009;138(4):738-49.

Wu G, Park MY, Conway SR, Wang JW, Weigel D, Poethig RS. The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis. Cell. 2009;138(4):750-9.

Baurle I, Dean C. The timing of developmental transitions in plants. Cell. 2006;125(4):655-64.

Schmid M, Uhlenhaut NH, Godard F, Demar M, Bressan R, Weigel D, Lohmann JU. Dissection of floral induction pathways using global expression analysis. Development. 2003;130(24):6001-12.

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Published

2017-03-07

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Chen Z, Lou J. Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra. Arch Biol Sci [Internet]. 2017Mar.7 [cited 2024Mar.29];69(1):93-101. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/379

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