Ultrasound-microwave assisted extraction of flavonoid compounds from Eucommia ulmoides leaves and an evaluation of their antioxidant and antibacterial activities
Keywords:Eucommia ulmoides leaves, flavonoids, ultrasound-microwave-assisted extraction, antioxidant activity, antibacterial activity
- The aim of this study is to improve the yield of flavonoid active ingredients from E. ulmoides leaves by establishing a high-performance extraction method.
- Ultrasound-microwave-assisted extraction was studied and the operational parameters were optimized by the Plackett-Burman and Box-Behnken design methods.
- Compared to conventional extraction methods and those using cell wall disruption, ultrasound-microwave-assisted extraction exhibited significantly high efficiency. Moreover, the obtained flavonoid extract exhibited prominent antioxidant and antibacterial activities.
- Ultrasound-microwave assisted extraction is a rapid and safe novel extraction technology, highly suitable for application in food and medical industries.
Abstract: Ultrasound/microwave assisted extraction (UMAE) of flavonoid compounds from Eucommia ulmoides leaves was studied and the extraction conditions were optimized by the Plackett-Burman design (PBD) method combined with the Box-Behnken design (BBD). The antioxidant and antibacterial activities of the flavonoid extract were investigated. The results show that the optimal conditions were an ethanol concentration of 41%, microwave power of 178 W and an ultrasound extraction time of 26 min. Under these conditions, the yield of the flavonoid compounds was 2.454%±0.230%, which was higher than that after direct solvent extraction, ultrasound extraction and microwave extraction. The results of in vitro antioxidant assays showed that the flavonoid extract had scavenging capacity for DPPH, ABTS and hydroxyl radicals, with corresponding IC50 values of 30.76 mg/L, 21.09 mg/L, 248.4 mg/L, respectively. In addition, this extract exhibited strong antibacterial activity on Escherichia coli, Staphylococcus aureus and Salmonella typhimurium.
Received: December 16, 2019; Revised: April 14, 2020; Accepted: April 14, 2020; Published online: April 21, 2020
How to cite this article: Wang X, Peng M, Wang Z, Yang Q, Peng S.Ultrasound-microwave assisted extraction of flavonoid compounds from Eucommia ulmoides leaves and an evaluation of their antioxidant and antibacterial activities. Arch Biol Sci. 2020;72(2):211-21.
Dai X, Huang Q, Zhou B, Gong Z, Liu Z, Shi S. Preparative isolation and purification of seven main antioxidants from Eucommia ulmoides Oliv. (Du-zhong) leaves using HSCCC guided by DPPH-HPLC experiment. Food Chem. 2013;139(1-4):563-70.
Xu JK, Li MF, Sun RC. Identifying the impact of ultrasound-assisted extraction on polysaccharides and natural antioxidants from Eucommia ulmoides Oliver. Process Biochem. 2015;50(3):473-81.
Deyama T, Nishibe S, Nakazawa Y. Constituents and pharmacological effects of Eucommia and Siberian ginseng. Acta Pharmacol Sin. 2001;22(12):1057-70.
Zhong SJ, Yang X, Li J, Li Y, Li XS. Study on the Total Flavonoids Content and Antioxidant Activity in Different Parts of Eucommiae ulmoides. China Pharmacy. 2017;28(13):1787-90.
Greenway F, Liu Z, Yu Y, Gupta A. A clinical trial testing the safety and efficacy of a standardized Eucommia ulmoides Oliver bark extract to treat hypertension. Altern Med Rev. 2011;16(4):338-47.
Tuba A, İlhami G. Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact. 2008;174:27–37.
Parvathy KS, Negi PS, Srinivas P. Antioxidant, antimutagenic and antibacterial activities of curcumin-β-diglucoside. Food Chem. 2008;115(1):265-71.
Rodríguez-Rojo S, Visentin A, Maestri D, Cocero M. Assisted extraction of rosemary antioxidants with green solvents. J Food Eng. 2012;109:98-103.
Mehmooda A, Ishaq M, Zhao L, Yaqoob S, Safdar B, Nadeeme M, Munir M, Wang C. Impact of ultrasound and conventional extraction techniques on bioactive compounds and biological activities of blue butterfly pea flower (Clitoria ternatea L.). Ultrason Sonochem. 2019;51:12-9.
Aurore F, Xavier F, Matteo M, Francesco V, Giancarlo C, Farid C. Solvent-free microwave extraction of essential oil from aromatic herbs: from laboratory to pilot and industrial scale. Food Chem. 2014;150(5):193-8.
Sandrine PI, Christian G, Giancarlo C, Farid C. A comparison of essential oil obtained from lavandin via different extraction processes: ultrasound, microwave, turbohydrodistillation, steam and hydrodistillation. J Chromatogra A. 2013;1305(24):41-7.
Cheung YC, Siu KC, Liu YS, Wu JY. Molecular properties and antioxidant activities of polysaccharide-protein complexes from selected mushrooms by ultrasound-assisted extraction. Process Biochem. 2012;47(5):892-5.
Ruciza JM, Slobodanka K, Eleonora W. Application of ultrasound for enhanced extraction of prebiotic oligosaccharides from selected fruits and vegetables. Ultrason Sonochem. 2015;22(22):446-53.
Li Y, Zeng R, Lu Q, Wu S, Chen J. Ultrasound/microwave-assisted extraction and comparative analysis of bioactive/toxic indole alkaloids in different medicinal parts of Gelsemium elegans Benth by ultra-high performance liquid chromatography with MS/MS. J Sep Sci. 2014;37(3):308-13.
Zhang L, Liu Z. Optimization and comparison of ultrasound/microwave assisted extraction (UMAE) and ultrasonic assisted extraction (UAE) of lycopene from tomatoes. Ultrason Sonochem. 2008;15(5):731-7.
Chen Y, Gu X, Huang SQ, Li J, Wang X, Tang J. Optimization of ultrasonic/microwave assisted extraction (UMAE) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities. Int J Biol Macromol. 2010;46(4):429-35.
Bai F, Wang J, Guo J. Optimization for ultrasound-microwave assisted extraction of pectin from jujube waste using response surface methodology. Adv J Food Sci Technol. 2015;7(3):144-53.
Liu Z, Qiao L, Yang F, Gu H, Yang L. Brönsted acidic ionic liquid based ultrasound-microwave synergistic extraction of pectin from pomelo peels. Int J Biol Macromol. 2017;94(Pt A):309-18.
Rahmanian N, Jafari SM, Wani TA. Bioactive profile, dehydration, extraction and application of the bioactive components of olive leaves. Trends Food Sci Tech. 2015;42(2):150-72.
Pan G, Yu G, Zhu C, Qiao J. Optimization of ultrasound-assisted extraction (UAE) of flavonoids compounds (FC) from hawthorn seed (HS). Ultrason Sonochem. 2012;19(3):486-90.
Pinela J, Prieto MA, Carvalho AM, Barreiro MF, Oliveira BPP, Barros L, Ferreira ICFR. Microwave-assisted extraction of phenolic acids and flavonoids and production of antioxidant ingredients from tomato: A nutraceutical-oriented optimization study. Sep Purif Technol. 2016;164:114-24.
Guo WQ, Meng ZH, Ren NQ. Optimization of key variables for the enhanced production of hydrogen by Ethanoligenens harbinense W1 using response surface methodology. Int J Hydrogen Energ. 2011;36(10):5843-48.
Gharibzahedi SMT, Smith B, GuoY. Ultrasound-microwave assisted extraction of pectin from fig (Ficus carica L.) skin: Optimization, characterization and bioactivity. Carbohyd Polym. 2019;222:114992.
Gharibzahedi SMT., Razavi S H, Mousavi M. Optimisation and kinetic studies on the production of intracellular canthaxanthin in fed-batch cultures of Dietzia natronolimnaea HS-1. Qual Assur Saf Crop. 2015;7(5):757-67.
He QH, Gong ZJ, An M, Li GZ. Optimization of enzymatic extraction technology for algal oligosaccharide by response surface methodology. China Brewing. 2019;38(11):59-62.
Li YB, Yu JB, Wu SY, Zhi AP. Determination of total flavonoids in Brazil green propolis capsule. J Food Safety and Quality. 2015;(8):3060-3.
Frankel EN, Meyer AS. The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidants. J Sci Food Agr. 2000; 80(13):1925–41.
Thaipong K, Boonprakob U, Crosby K. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal. 2012;19(6):669-75.
Huang XY, Li SL, Dang CZ, Song QY, Yang QX. Study on antioxidative activity and ingredient of extract from Toona Sinensis fruit. Seeds. 2011;30(7):79-82.
Fazeli MR, Amin G, Attari MMA, Ashtiani H, Jamalifar H, Samadi N. Antimicrobial activities of Iranian sumac and avishan-e shirazi (Zataria multiflora) against some food-borne bacteria. Food Control. 2007;18(6):646-9.
Zhang CW, Wang CZ, Fang C. Enzymolysis-based ultrasound extraction and antioxidant activities of polyprenol lipids from Ginkgo biloba leaves. Process Biochem. 2016;51(3):444-51.
Liu GP, Jiao FF. Study on the ultrasonic assisted extraction of total flavonoids from Eucommia ulmoides leaves. Food Ferment Ind. 2013;39(11):258-61.
Liu GP, Cui BJ, Sun JY, Gao XL. Microwave-assisted extraction process of total flavonoids from Eucommia ulmoides leaves. China Brewing. 2015;34(5):127-30.
Zhang G, He L, Hu M. Optimized ultrasonic-assisted extraction of flavonoids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro. Innov Food Sci Emerg Technol. 2011;12:18-25.
Lu X, Zheng Z, Li H, Cao R, Zheng YF, Yue H, Xiao JB, Miao S, Zheng BD. Optimization of ultrasonic-microwave assisted extraction of oligosaccharides from lotus (Nelumbo nucifera, Gaertn.). Ind Crop Prod. 2017;107:546-57.
Alupului A, Călinescu I, Lavric V. Microwave extraction of active principles from medicinal plants. U P B Sci Bull Series B. 2012;74:129-42.
Gharibzahedi SMT. Ultrasound-mediated nettle oil nanoemulsions stabilized by purified jujube polysaccharide: Process optimization, microbial evaluation and physicochemical storage stability. J Mol Liq. 2017;234:240-8.
Moorthy IG, Maran JP, Muneeswari S, Naganyashree S, Shivamathi CS. Response surface optimization of ultrasound assisted extraction of pectin from pomegranate peel. Int J Biol Macromol. 2015;72:1323-8.
Prakash MJ, Sivakumar V, Thirugnanasambandham K, Sridhar R. Microwave assisted extraction of pectin from waste Citrullus lanatus fruit rinds. Carbohyd Polym. 2014;101:786-91.
Liew SQ, Ngoh GCH, Yusoff R, Teoh WH. Sequential ultrasound-microwave assisted acid extraction (UMAE) of pectin from pomelo peels. Int J Biol Macromol. 2016;93:426-35.
Wizi J, Wang L, Hou XL, Tao YY, Ma B, Yang YQ. Ultrasound-microwave assisted extraction of natural colorants from sorghum husk with different solvents. Ind Crop Prod. 2018;120:203-13.
Wang ZJ. Xie JH, Kan LJ, Wang J., Shen MY, Li WJ, Nie SP, Xie MY. Sulfated polysaccharides from Cyclocarya paliurus reduce H2O2-induced oxidative stress in RAW264.7 cells. Int J Biol Macromol. 2015;80:410-7.
Madian AG, Regnier FE. Proteomic identification of carbonylated proteins and their oxidation sites. J Proteome Res. 2010;9:3766-80.
Ouyang H, Hou K, Peng WX, Liu ZL, Deng HP. Antioxidant and xanthine oxidase inhibitory activities of total polyphenols from onion. Saudi J Biol Sci. 2018;25:1509-13.
Hou MY, Hu WZ, Xiu ZL, Shi YS, Hao KX, Cao D, Guan YG, Yin HL. Efficient enrichment of total flavonoids from Pteris ensiformis Burm. extracts by macroporous adsorption resins and in vitro evaluation of antioxidant and antiproliferative activities. J Chromatogr B. 2020;1138:121960.
Trouillas P, Marsal P, Siri D, Lazzaroni R, Duroux JL. A DFT study of the reactivity of OH groups in quercetin and taxifolin antioxidants: the specificity of the 3-OH site. Food Chem. 2006;97(4):679-88.
Tsimogiannis DI, Oreopoulou V. The contribution of flavonoid C-ring on the DPPH free radical scavenging efficiency. A kinetic approach for the 3′, 4′-hydroxy substituted members. Innov Food Sci Emerg. 2006;7(1):140-6.
Hamed YS, Abdin M, Akhtar HMS, Chen D, Wan P, Chen G, Zeng X. Extraction, purification by macrospores resin and in vitro antioxidant activity of flavonoids from Moringa oliefera leaves. S Afr J Bot. 2019;124:270-9.
Li X, Liu T. Optimization of microwave-assisted extraction of flavonoids from Eucommia ulmoides leaf and investigation of their antioxidative effect. Sci-Tech Food Ind. 2013;34(04):243-8.
Wannissorn B, Jarikasem S, Siriwangchai T, Thubthimthed S. Antibacterial properties of essential oils from Thai medicinal plants. Fitoterapia. 2005;76:233-6.
Ji ZP, Su YQ. Study on antimicrobial activities of extracts from Eucommia ulmoides Oliv. leaves. Chem Ind Forest Prod. 2008;28(2):63-6.
Gill AO, Holley RA. Disruption of Escherichia coli, Listeria monocytogenes and Lactobacillus sakei cellular membranes by plant oil aromatics. Int. J. Food Microbiol. 2006.;108:1-9.
Guo N, Zang YP, Cui Q, Gai QY, Jiao J, Wang W, Zu YG, Fu YJ. The preservative potential of Amomum tsaoko essential oil against E. coil, its antibacterial property and mode of action. Food Control. 2017;75:236-45.