Ultrasound-microwave assisted extraction of flavonoid compounds from Eucommia ulmoides leaves and an evaluation of their antioxidant and antibacterial activities

Authors

  • Xiang Wang 1. National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia ulmoides, Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie; 2. Institute of Chemical Industry of Forest Products, CAF; National Engineering Lab for Biomass Chemical Utilization; Key and Open Laboratory of Forest Chemical Engineering, SFA; Key Laboratory of Biomass Energy and Material, Nanjing; 3. Research Institute of Forestry New Technology, CAF, Beijing; 4. Guangdong Provincial Public Laboratory of Analysis and Testing Technology, Guangdong Institute of Analysis, Guangzhou http://orcid.org/0000-0001-7206-5661
  • Mi-Jun Peng Guangdong Provincial Public Laboratory of Analysis and Testing Technology, Guangdong Institute of Analysis, Guangzhou http://orcid.org/0000-0003-4886-445X
  • Zhi-Hong Wang Guangdong Provincial Public Laboratory of Analysis and Testing Technology, Guangdong Institute of Analysis, Guangzhou http://orcid.org/0000-0001-7418-5130
  • Qiu-Ling Yang Guangdong Provincial Public Laboratory of Analysis and Testing Technology, Guangdong Institute of Analysis, Guangzhou http://orcid.org/0000-0003-1037-0171
  • Sheng Peng National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia ulmoides, Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie http://orcid.org/0000-0003-4115-5335

Keywords:

Eucommia ulmoides leaves, flavonoids, ultrasound-microwave-assisted extraction, antioxidant activity, antibacterial activity

Abstract

Paper description:

  • The aim of this study is to improve the yield of flavonoid active ingredients from E. ulmoides leaves by establishing a high-performance extraction method.
  • Ultrasound-microwave-assisted extraction was studied and the operational parameters were optimized by the Plackett-Burman and Box-Behnken design methods.
  • Compared to conventional extraction methods and those using cell wall disruption, ultrasound-microwave-assisted extraction exhibited significantly high efficiency. Moreover, the obtained flavonoid extract exhibited prominent antioxidant and antibacterial activities.
  • Ultrasound-microwave assisted extraction is a rapid and safe novel extraction technology, highly suitable for application in food and medical industries.


Abstract: Ultrasound/microwave assisted extraction (UMAE) of flavonoid compounds from Eucommia ulmoides leaves was studied and the extraction conditions were optimized by the Plackett-Burman design (PBD) method combined with the Box-Behnken design (BBD). The antioxidant and antibacterial activities of the flavonoid extract were investigated. The results show that the optimal conditions were an ethanol concentration of 41%, microwave power of 178 W and an ultrasound extraction time of 26 min. Under these conditions, the yield of the flavonoid compounds was 2.454%±0.230%, which was higher than that after direct solvent extraction, ultrasound extraction and microwave extraction. The results of in vitro antioxidant assays showed that the flavonoid extract had scavenging capacity for DPPH, ABTS and hydroxyl radicals, with corresponding IC50 values of 30.76 mg/L, 21.09 mg/L, 248.4 mg/L, respectively. In addition, this extract exhibited strong antibacterial activity on Escherichia coli, Staphylococcus aureus and Salmonella typhimurium.

https://doi.org/10.2298/ABS191216015W

Received: December 16, 2019; Revised: April 14, 2020; Accepted: April 14, 2020; Published online: April 21, 2020

How to cite this article: Wang X, Peng M, Wang Z, Yang Q, Peng S.Ultrasound-microwave assisted extraction of flavonoid compounds from Eucommia ulmoides leaves and an evaluation of their antioxidant and antibacterial activities. Arch Biol Sci. 2020;72(2):211-21.

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Published

2020-07-01

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Wang X, Peng M-J, Wang Z-H, Yang Q-L, Peng S. Ultrasound-microwave assisted extraction of flavonoid compounds from Eucommia ulmoides leaves and an evaluation of their antioxidant and antibacterial activities. Arch Biol Sci [Internet]. 2020Jul.1 [cited 2022Aug.7];72(2):211-2. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4921

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