Effects of kinetin on biological parameters and hemocytes of Achroia grisella (Lepidoptera: Pyralidae)



Achroia grisella, cytokinin, life history traits, morphological disorder, hemocyte number


Paper description:

  • Our aim was to improve our understanding of the influence of plant growth regulators on insect biology and physiology.
  • Kinetin effects were examined for the life history traits and hemocytes of Achroia grisella by addition to moth diet or direct injection to the final instars.
  • We show that kinetin can interfere with normal developmental and immune processes of A. grisella by reducing weight, progeny and hemocyte counts.
  • Impairment of the immune system and biological characteristics reveal that kinetin can be used to control this pest, however, long-term harmful effects may develop in both host and parasitoid species.

Abstract: The effects of the cytokinin hormone kinetin on the life history traits and hemocytes of the smaller wax moth Achroia grisella F. (Lepidoptera: Pyralidae) were examined in order to better understand the physiological impacts of plant growth regulators on insects. Based on the obtained results, it was found that kinetin did not lead to significant changes in larval mortality, development time, morphological disorders and egg fertility. Female and male longevity were almost unchanged when early instars were fed with a kinetin-added diet, and it tended to be higher for males when kinetin was applied at the egg stage, especially at 5 mg/L. The weight of females decreased significantly with 25 and 3000 mg/L of kinetin and that of males with 5 mg/L. The most striking effect was a considerable decline in the number of progeny, particularly at 400 and 3000 mg/L, as compared to the control. The injection of kinetin caused noticeable decreases in the number of hemocytes in the circulation at 3 and 24 h. This work presents the first evidence that kinetin adversely affects the development and the hemocyte counts of an economically-important host species, A. grisella.


Received: January 7, 2020; Revised: March 20, 2020; Accepted: March 27, 2020; Published online: April 8, 2020

How to cite this article: Çelik E, Sak O. Effects of kinetin on biological parameters and hemocytes of Achroia grisella (Lepidoptera: Pyralidae). Arch Biol Sci. Arch Biol Sci. 2020;72(2):181-92.


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Miller CO, Skoog F, Okumura FS, Von Saltza MH, Strong FM. Isolation, structure and synthesis of kinetin, a substance promoting cell division. J Am Chem Soc. 1956;78(7):1375-80.

Rattan SIS. N6-furfuryladenine (kinetin) as a potential anti-aging molecule. J Anti-Aging Med. 2002;5(1):113-6.

Miller CO, Skoog F, Von Saltza MH, Strong FM. Kinetin, a cell division factor from deoxyribonucleic acid. J Am Chem Soc. 1955;77:1392.

Amasino R. 1955: Kinetin arrives. The 50th anniversary of a new plant hormone. Plant Physiol. 2005;138:1177-84.

US-EPA: US Environmental Protection Agency Office of Pesticide Programs Biopesticides and Pollution Prevention Division [Internet]. Washington (PA): Kinetin technical. 2016 - [cited 2016 Jul 25]. Available from: https://www3.epa.gov/pesticides/chem_search/ppls/091620-00002-20160725.pdf.

Raman N, Elumalai S. Presence of cytokinin in the root nodules of Casuarina equisetifolia. Ind J Exp Biol. 1996;34:577-80.

Ratti N, Janardhanan KK. Effect on growth, phosphorus and cytokinin contents of palmarosa (Cymbopogon martini var. motia) by Glomus inoculation. Ind J Exp Biol. 1996;34:1126-28.

Barciszewski J, Siboska GE, Pedersen BO, Clark BFC, Rattan SIS. Evidence for the presence of kinetin in DNA and cell extracts. FEBS Lett. 1996;393:197-200.

Barciszewski J, Mielcarek M, Stobiecki M, Siboska G, Clark BFC. Identification of 6-furfuryladenine (kinetin) in human urine. Biochem Bioph Res Co. 2000;279(1):69-73.

Barciszewski J, Rattan SIS, Siboska G, Clark BFC. Kinetin-45 years on. Plant Sci. 1999;148(1):37-45.

Barciszewski J, Massino F, Clark BFC. Kinetin-A multiactive molecule. Int J Biol Macromol. 2007;40(3):182-92.

Sharma SP, Kaur P, Rattan SIS. Plant growth hormone kinetin delays aging, prolongs the lifespan, and slows down development of the fruitfly Zaprionus paravittiger. Biochem Bioph Res Co. 1995;216(3):1067-71.

Sharma SP, Kaur J, Rattan SIS. Increased longevity of kinetin-fed Zaprionus fruitflies is accompanied by their reduced fecundity and enhanced catalase activity. Biochem Mol Biol Int. 1997;41(5):869-75.

Olsen A, Siboska GE, Clark BFC, Rattan SIS. N6-furfuryladenine, kinetin, protects against Fenton reaction-mediated oxidative damage to DNA. Biochem Bioph Res Co. 1999;265(2):499-502.

Ahanger MA, Alyemeni MN, Wijaya L, Alamri SA, Alam P, Ashraf M, Ahmad P. Potential of exogenously sourced kinetin in protecting Solanum lycopersicum from NaCl-induced oxidative stress through up-regulation of the antioxidant system, ascorbate-glutathione cycle and glyoxalase system. PloS ONE. 2018;13(9):1-21.

Mccullough JL, Weinstein GD. Clinical study of safety and efficacy of using topical kinetin 0.1% (Kinerase®) to treat photodamaged skin. Cosmet Dermatol. 2002;15(9):29-32.

Wu JJ, Weinstein GD, Kricorian GJ, Kormeili T, McCullough JL. Topical kinetin 0.1% lotion for improving the signs and symptoms of Rosacea. Clin Exp Dermatol. 2007;32(6):693-5.

Miastkowska M, Sikora E. Anti-aging properties of plant stem cell extracts. Cosmetics. 2018;5(4):55.

Visscher SN. Plant growth hormones affect grasshopper growth and reproduction. In: Visser JH, Minks AK, editors. Proceedings of the 5th International Symposium of Insect-Plant Relationships. Wageningen: Pudoc, 1982. p. 57-62.

Chawla SS, Perron JM, Cloutier M. Effects of different growth factors on the potato aphid, Macrosiphum euphorbiae (Aphididae: Homoptera), fed on an artificial diet. Can Entomol. 1974;106(3):273-80.

Rup PJ, Sohal SK, Kaur P, Kaur S, Dhillon MK, Kumari J, Dhingra P. Population studies on the mustard aphid, Lipaphis erysimi (Kalt.) in response to 14 plant growth regulators. J Aphidol. 1998;12:67-73.

Rup PJ, Sohal SK, Kaur G, Sharma R. The possible role of five enzymes in the metabolism of kinetin in mustard aphid, Lipaphis erysimi (Kaltenbach). J Aphidol. 2002;16:1-8.

Rup PJ, Sohal SK, Kaur H. Studies on the role of six enzymes in the metabolism of kinetin in mustard aphid, Lipaphis erysimi (Kalt.). J Environ Biol. 2006;27(3):579-84.

Sepperumal U, Sukumar S. Impact of kinetin and gibberellic acid on the commercial characteristics of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). J Microbiol Biotech Res. 2014;4(1):11-20.

Yeşilada E, Bozcuk AN. The effects of ABA and kinetin on the fecundity of Drosophila melangaster. Turk J Biol. 1995;19:37-44.

Yeşilada E, Bozcuk AN. The effects of ABA and kinetin on the developmental period of Drosophila melanogaster. Turk J Biol. 1996;20:29-35.

Yeşilada E, Bozcuk AN, Bozcuk S, Topcuoğlu F. The effects of abscisic acid (ABA) and kinetin on sex-ratio and adult morphology of D. melanogaster. Turk J Biol. 1996;20:171-8.

Yeşilada E. The effect of kinetin, gibberellic acid and indole acetic acid on EMS-induced somatic mutation and recombination in Drosophila melanogaster. Turk J Biol. 2000;24(2):279-84.

Kaur R, Rup PJ. Evaluation of regulatory influence of four plant growth regulators on the reproductive potential and longevity of melon fruit fly (Bactrocera cucurbitae). Phytoparasitica. 2002;30(3):224-30.

Kaur R, Rup PJ. Influence of four plant growth regulators on development of the melon fruit fly, Bactrocera cucurbitae (Coquillett). Insect Sci Appl. 2003a;23(2):121-5.

Kaur R, Rup PJ. Influence of some plant growth regulators (PGR) on biochemical profile in the larvae of melon fruit fly Bactrocera cucurbitae (Coquillett) (Diptera: Trypetidae). Entomon. 2003b;28(2):89-95.

Uçkan F, Hepçorman Şengül Ş, Sak O, Korkmaz M. Effects of 5-Aza-2′-deoxycytidine on biological parameters of larval endoparasitoid Apanteles galleriae (Hymenoptera: Braconidae) and on its host Achoria grisella (Lepidoptera: Pyralidae). Ann Entomol Soc Am. 2007;100(2):265-69.

Ergin E, Er A, Uçkan F, Rivers DB. Effect of cypermethrin exposed hosts on egg-adult development time, number of offspring, sex ratio, longevity, and size of Apanteles galleriae Wilkinson (Hymenoptera: Braconidae). Belg J Zool. 2007;137(1):27-31.

Uçkan F, Tüven A, Er A, Ergin E. Effects of gibberellic acid on biological parameters of the larval endoparasitoid Apanteles galleriae (Hymenoptera: Braconidae). Ann Entomol Soc Am. 2008;101(3):593-7.

Uçkan F, Haftacı İ, Ergin E. Effects of indol-3-acetic acid on biological parameters of the larval endoparasitoid Apanteles galleriae (Hymenoptera: Braconidae). Ann Entomol Soc Am. 2011a;104(1):77-82.

Çelik D, Özbek R, Uçkan F. Effects of indole-3-acetic acid on hemocytes of Achoria grisella Fabr. (Lepidoptera: Pyralidae). J Entomol Res Soc. 2017;19(2):83-93.

Sak O. The effects of 5-Aza-2´-deoxycytidine on total lipid and fatty acid composition of Apanteles galleriae Wilkinson (Hymenoptera: Braconidae) and on its parasitized host. J Entomol Res Soc. 2017;19(1), 105-20.

Sak O. Effects of 5-aza-2´-deoxycytidine on Biological Parameters of Achroia grisella F. (Lepidoptera: Pyralidae). Arch Biol Sci. 2018;70(1):149-58.

Zibaee A. Botanical Insecticides and Their Effects on Insect Biochemistry and Immunity. In: Stoytcheva M, editor. Pesticides in the modern world - pests control and pesticides exposure and toxicity assessment. Rijeka: InTech; 2011. p. 55-68.

Venier P, Maron S, Canova S. Detection of micronuclei in gill cells and haemocytes of mussels exposed to benzo[a]pyrene. Mutat Res. 1997;390:33-44.

James RR, Xu J. Mechanisms by which pesticides affect insect immunity. J Invertebr Pathol. 2012;109(2):175-82.

Gupta AP. Cellular Elements in the Hemolymph. In: Kerkut GA, Gilbert LI, editors. Comprehensive insect physiology biochemistry and pharmacology, Vol 3. New York: Pergamon Press; 1985. p. 401-51.

Altuntaş H, Kılıç AY, Uçkan F, Ergin E. Effects of gibberellic acid on hemocytes of Galleria mellonella L. (Lepidoptera: Pyralidae). Environ Entomol. 2012;41(3):688-96.

Er A, Keskin M. Influence of abscisic acid on the biology and hemocytes of the model insect Galleria mellonella (Lepidoptera: Pyralidae). Ann Entomol Soc Am. 2016;109(2):244-51.

Uçkan F, Gülel A. Effects of host species on some biological characteristics of Apanteles galleriae Wilkinson (Hymenoptera; Braconidae). Turk J Zool. 2000;24:105-13.

Uçkan F, Ergin E. Temperature and food source effects on adult longevity of Apanteles galleriae Wilkinson (Hymenoptera: Braconidae). Environ Entomol. 2003;32(3):441-6.

Bronskill JF. A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pyralidae). J Lep Soc. 1961;15(2):102-4.

Sak O, Uçkan F, Ergin E. Effects of cypermethrin on total body weight, glycogen, protein, and lipid contents of Pimpla turionellae (L.) (Hymenoptera: Ichneumonidae). Belg J Zool. 2006;136(1):53-8.

Lincoln DE, Couvet D, Sionit N. Response of an insect herbivore to host plants grown in carbon dioxide enriched atmospheres. Oecologia. 1986;69(4):556-60.

Er A, Uçkan F, Rivers DB, Ergin E, Sak O. Effects of parasitization and envenomation by the endoparasitic wasp Pimpla turionellae (Hymenoptera: Ichneumonidae) on hemocyte numbers, morphology, and viability of its host Galleria mellonella (Lepidoptera: Pyralidae). Ann Entomol Soc Am. 2010;103(2):273-82.

World Health Organization (WHO): 2,4-Dichlorophenoxyacetic acid (2,4-D). Geneva: World Health Organization; 1984. 151 p. (Environmental health criteria; 29).

Guedes RNC, Smagghe G, Stark JD, Desneux N. Pesticide-induced stress in arthropod pests for optimized integrated pest management programs. Ann Rev Entomol. 2016;61:43-62.

Pijpe J, Fischer K, Brakefield PM, Zwaan BJ. Consequences of artificial selection on pre-adult development for adult lifespan under benign conditions in the butterfly Bicyclus anynana, Mech Ageing Dev. 2006;127(10):802-7.

Uçkan F, Özbek R, Ergin E. Effects of indol-3-Acetic Acid on the biology of Galleria mellonella and its endoparasitoid Pimpla turionellae. Belg J Zool. 2015;145(1):49-58.

Uçkan F, Öztürk Z, Altuntaş H, Ergin E. Effects of gibberellic acid (GA3) on biological parameters and hemolymph metabolites of the pupal endoparasitoid Pimpla turionellae (Hymenoptera: Ichneumonidae) and its host Galleria mellonella (Lepidoptera: Pyralidae). J Entomol Res Soc. 2011b;13(3):1-14.

Kaur M, Kaur S. Tritrophic interactions among coumarin, the herbivore Spodoptera litura and a gregarious ectoparasitoid Bracon hebetor. Biol Control. 2013;58:755-63.

Prado SG, Frank SD. Tritrophic effects of plant growth regulators in an aphid-parasitoid system. Biol Control. 2013;66:72-6.

Altuntaş H, Uçkan F, Kılıç AY, Ergin E. Effects of gibberellic acid on hemolymph-free amino acids of Galleria mellonella (Lepidoptera: Pyralidae) and endoparasitoid Pimpla turionellae (Hymenoptera: Ichneumonidae). Ann Entomol Soc Am. 2014;107(5):1000-9.

Uçkan F, Soydabaş HK, Özbek R. Effect of indol-3 acetic acid on the biochemical parameters of Achoria grisella hemolymph and Apanteles galleriae larva. Pak J Biotechnol. 2014;11(2):63-71.

Özcan Ö, Gündüz EA. Influence of gibberellic acid on some biological parameters of ectoparasitoid, Bracon hebetor (Say, 1836) (Hymenoptera: Braconidae). Acta Biologica Turcica. 2018;31(1):13-7.

Jiravanichpaisal P, Lee BL, Söderhäll K. Cell-mediated immunity in arthropods: hematopoiesis, coagulation, melanization and opsonization. Immunobiology. 2006;211:213-36.




How to Cite

Çelik E, Sak O. Effects of kinetin on biological parameters and hemocytes of Achroia grisella (Lepidoptera: Pyralidae). Arch Biol Sci [Internet]. 2020Jul.1 [cited 2022Aug.7];72(2):181-92. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4986