Effects of β-sitosterol on growth, development and midgut enzymes of Helicoverpa armigera Hübner

Authors

Keywords:

Helicoverpa armigera, β-sitosterol, alanine aminotransaminase, aspartate aminotransaminase, alkaline phosphatase

Abstract

Paper description:

  • Helicoverpa armigera is a global agricultural pest of serious concern. As repeated field application of chemical insecticides has caused negative impacts on the human health, non-targets and the environment, the effects of β-sitosterol, an eco-friendly bioactive phytocomponent, was investigated on H. armigera. larvae.
  • Different concentrations of β-sitosterol were incorporated in the larval diet to assess the effects on larval growth and development, and midgut enzymes.
  • Dietary β-sitosterol inhibited larval growth, affecting later instars and pupae more, pointing to cumulative effects of β-sitosterol.
  • β-sitosterol can be used in Helicoverpa management programs.


Abstract: Helicoverpa armigera is a global agricultural pest of serious concern. Continued use of chemical insecticides as control measures has raised grave health and environment concerns, necessitating a search for botanicals as safe alternatives. The current study investigates the effects of β-sitosterol, a bioactive phytocomponent in Thevetia neriifolia, on the growth and development, as well as on midgut enzymes of H. armigera. Dietary β-sitosterol produced dose-dependent systemic toxicity and growth inhibitory effects in H. armigera; the most significant effects were obtained with 10 µg/mL dietary β-sitosterol. Higher prepupal and pupal mortality in comparison to larval mortality and a comparatively greater reduction in average weight gained by later instars point to cumulative effects of β-sitosterol. The delayed effects were ascertained by the 82.05%-57.89% reduction in adult emergence in comparison to 95.02% emergence in controls. Dose-dependent effects of β-sitosterol were observed as significantly decreased enzyme activities of alanine aminotransaminase (ALT), aspartate aminotransaminase (AST) and alkaline phosphatase (ALP) in the larval midgut. Suppression of enzyme activity was obtained in the order ALT>AST>ALP. Impaired activity of gut enzymes possibly lowered the energy reserves and affected nutrient transport through the gut epithelium, affecting the growth and development of H. armigera. Our study points to a promising use of β-sitosterol against H. armigera, although further examination and field studies are needed to ascertain its possible use in control programs.

https://doi.org/10.2298/ABS200308021M

Received: March 8, 2020; Revised: April 28, 2020; Accepted: May 8, 2020; Published online: May 12, 2020

How to cite this article: Mishra M, Sharma A, Dagar VS, Kumar S. Effects of β-sitosterol on growth, development and midgut enzymes of Helicoverpa armigera Hübner. Arch Biol Sci. 2020;72(2):271-8.

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References

Kriticos DJ, Ota N, Hutchison WD, Beddow J, Walsh T, Tay WT, Borchert DM, Silvana Paula-Moreas SV, Czepak C, Zalucki MP. The potential distribution of invading Helicoverpa armigera in North America: Is it just a matter of time? Plos One. 2015;10(3):1-24.

Packiam SM, Baskar K, Ignacimuthu S. Ovicidal activity of botanical oil formulations against Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Int J Agric Tech. 2013;9(3):553-63.

Cunningham JP, Zalucki MP. Understanding heliothine (Lepidoptera: Heliothinae) pests: what is a host plant? J Econ Entomol. 2014;107(3):881-96.

Mishra M, Gupta KK, Kumar S. Growth regulatory and growth inhibitory effects of Thevetia neriifolia stem extracts on Helicoverpa armigera (Lepidoptera: Noctuidae). Arch Phytopathol Plant Prot. 2018;51:895-914.

Armes NJ, Jadhav DR, De Souse KR. A survey of insecticide resistance in Helicoverpa armigera in the Indian subcontinent. Bull Entomol Res. 1996;86(5):499-514.

Kranthi KR, Jadhav D, Wanjari R, Kranthi S, Russell D. Pyrethroid resistance and mechanisms of resistance in field strains of Helicoverpa armigera (Lepidoptera: Noctuidae). J Econ Entomol. 2001;94:253-63.

Vila TLM, Rodriguez MMC, Lacasa PA, Bielza LP. Insecticide resistance of Helicoverpa armigera to endosulfan, carbamates and organophosphates: The Spanish case. J Crop Prot. 2002;21(10):1003-13.

Thibaud M, Ochou OG, Maurice V, Didier F. Organophosphorus insecticides synergize pyrethroids in the resistant strain of cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) from West Africa. J Econ Entomol. 2003;96(2):468-74.

Yang Y, Li Y, Wu Y. Current status of insecticide resistance in Helicoverpa armigera after 15 years of Bt cotton planting in China. J Econ Entomol. 2013;106(1):375-81.

Tossou E, Tepa-Yotto G, Kpindou OKD, Sandeu R, Datinon B, Zeukeng F, Akoton R, Tchigossou GM, Djègbè I, Vontas J, Martin T, Wondji C, Tamò M, Bokonon-Gant AH, Djouaka R. Susceptibility profiles of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) to deltamethrin reveal a contrast between the Northern and the Southern Benin. Int J Environ Res Publ Hlth. 2019;16:1-15.

Williams LAD. Rhizophora mangle (Rhizophoraceae) triterpenoids with insecticidal activity. Naturwissenschaften. 1999;86:450-2.

Hikal WM, Baeshen RS, Said-Al Ahl HAH. Botanical insecticide as simple extractives for pest control. Cogent Biol. 2017;3:1-16.

Mishra M, Gupta KK, Kumar S. Impact of the stem extract of Thevetia neriifolia (Apocynaceae) on food consumption and growth of early fourth instars of Helicoverpa armigera. Malaya J Biosci. 2015;2(1):26-35.

Mishra M, Gupta KK, Kumar S. Impact of the stem extract of Thevetia neriifolia on the feeding potential and histological architecture of the midgut epithelial tissue of early fourth instars of Helicoverpa armigera Hübner. Int J Insect Sci. 2015;7:53-60.

Mishra M, Gupta KK, Kumar S. Diminished activity of larval midgut transaminases and phosphatases in Helicoverpa armigera Hübner (Lepidoptera) induced by dietary stem extracts of Thevetia neriifolia. J Lep Soc. 2019;73(1):23-33.

Hamadah KS. Disturbance of phosphatase and transaminase activities in grubs of the red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) by certain insecticidal compounds. J Basic Appl Zool. 2019;80:1-8.

Wolfersberger MG. Enzymology of plasma membranes of insect intestinal cells. Am Zool. 1984;24:187-97.

Dadd RH. Arthropoda nutrition. In: Florkin M, Scheer Bt, editors. Chemical Zoology. Vol 5, Arthropoda, Part A. New York: Academic Press;1970. p. 35-95.

Mordue W, Goldworthy GJ. Transaminase levels and uric acid production in adult locusts. Insect Biochem. 1973;3:419-27.

Adel MM, El-Hawary FM, Abdel-Aziz NF, Sammour EA. Some physiological, biochemical and histopathological effects of Artemisia monosperma against the cotton leafworm, Spodoptera littoralis. Arch Phytopathol Pflanzenschutz. 2010;43(11):1098-110.

Salim AS. Identification of active pharmaceutical ingredients in Thevetia neriifolia juss leaf callus using analysis of GC-MS. Indian J Public Health Res Dev. 2018;9(12):1019-23.

Saeidnia S, Manayi A, Gohari AR, Abdollahi M. The Story of Beta-sitosterol-A Review. Eur J Med Plant. 2014;4(5):590-609.

Ritter FJ, Wientjens WHJM. Sterol metabolism in insects. TNO Nieuws. 1967; 22:381-92.

Robbins WE, Dutky RC, Monroe RE, Kaplanis JN. The metabolism of 3H-β-sitosterol by the German cockroach. Ann Entomol Soc Am. 1962; 55:102-4.

Svoboda JA, Hutchins RFN, Thompson MJ, Robbins WE. 22-Trans-cholesta-5,22,24-trien3β-ol-An intermediate in the conversion of stigmasterol to cholesterol in the tobacco hornworm, Manduca sexta (Johannson). Steroids. 1969;14:469-76.

Hamamura Y, Hayashiya K, Naito K. Food selection by silkworm larvae, Bombyx mori: β-sitosterol as one of the biting factors. Nature. 1961;190:180-1.

Levinson ZH. The function of dietary sterols in phytophagous insects. J Ins Physiol. 1962;8(2):191-8.

Guozhou Z, Yawei W, Hanhong X, Shanhuan Z. Physio-biochemical effects on insects by beta-sitosterol, Daphnoritin and Chamechromone. J Hunan Agric Univ. 2000;26(5):366-7.

Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K. Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet. Parasitol Res. 2008;102:981-8.

Bradford MA. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54 .

Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957;28:56-63.

King PRH, EJ King. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol. 1954;7:322-6.

Adeyemi MMH. The potential of secondary metabolites in plant material as deterrents against insect pests: A review. Afr J Pure Appl Chem. 2010;4(11):243-6.

Pavunraj M, Baskar K, Ignacimuthu S. Efficacy of Melochia corchorifolia L. (Sterculiaceae) on feeding behavior of four lepidopteran pests. Int J Agric Res. 2012;7:58-68.

Diaz M, Castillo L, Díaz CE, Álvarez RG, González-Coloma A, Rossini C. Differential deterrent activity of natural products isolated from Allophylus edulis (Sapindaceae). Adv Biol Chem. 2014;4:168-79.

Varitimidis C, Petrakis PV, Vagias C, Roussis V. Secondary metabolites and insecticidal activity of Anemone pavonina. Zeitschrift für Naturforschung. 2006;61:521-6.

Kannan S, Vijayakumar B, Sureshkumar C, Mohankumar R, Narasimhan S. Insect antifeedant and growth regulating activities of β-amyrin from Sarcostemma acidum. Asian J Chem. 2013;25(2):1167-8.

Singh D, Mehta SS, Neoliya KN, Shukla NY, Mishra M. New possible insect growth regulators from Catharanthus roseus. Curr Sci. 2003;84:1184-6.

Pavela R, Zabka M, Tylova T, Kresinova Z. Insecticidal activity of compounds from Ailanthus altissima against Spodoptera littoralis larvae. Pak J Agr Sci. 2014;51(1):101-12.

Loh FS, Awang RM, Omar D, Rahmani M. Insecticidal properties of Citrus hystrix DC leaves essential oil against Spodoptera litura Fabricius. J Med Plant Res. 2011;5(16):3739-44.

Jadhav DR, Mallikarjuna N, Rathore A, Pokle D. Effect of some flavonoids on survival and development of Helicoverpa armigera (Hübner) and Spodoptera litura (Fab) (Lepidoptera: Noctuidae). Asian J Agric Sci. 2012;4(4):298-307.

Eguchi M. Alkaline phosphatase isozymes in insects and comparison with mammalian enzyme. Comp Biochem Physiol B. 1995;111:151-62.

Yan Y, Peng L, Liu WX, Wan FH. Research progress in insect alkaline phosphatases. Acta Entomol Sin. 2009;1:95-105.

Etebari K, Matindoost L. Effects of hypervitaminosis of vitamin B3 on silkworm biology. J Biosci. 2004;29:417-22.

Etebari K, Bizhannia AR, Sorati R, Matindoost L. Biochemical changes in haemolymph of silkworm larvae due to pyriproxyfen residue. Pestic Biochem Phys. 2007;88:14-9.

Senthil-Nathan S, Chung PG, Murugan K. Effect of botanical insecticides and bacterial toxins on the gut enzyme of the rice leaf folder Cnaphalocrocis medinalis. Phytoparasitica. 2004;32:433-43.

Abou-Taleb HK, Hossam El-Din ZM, Abir AG. Biochemical and physiological effects of lufenuron and chlorfluazuron on Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). J Entomol. 2015;12:77-86.

Miao Y. Studies on the activity of the alkaline phosphatase in the midgut of infected silkworm, Bombyx mori L. J Appl Entomol. 2002;126:138-42.

Zera A J, Zhao Z. Effect of a juvenile hormone analogue on lipid metabolism in a wing-polymorphic cricket: implications for the endocrine biochemical bases of life-history trade-offs. Physiol Biochem Zool. 2004;77:255-66.

Abd El-Aziz MF, El-Sayed YYA. Toxicity and biochemical efficacy of six essential oils against Tribolium confusum (du val) (Coleoptera: Tenebrionidae). Egypt Acad J Biol Sci. 2009;2:1-11.

Al-Dali AG. Influenced activities of the intestine acid and alkaline phosphatases by some neem limonoids in the grasshopper Euprepocnemis plorans (Orthoptera: Acrididae). J Egypt Acad Soc Environ Dev. 2007;8(3):57-65.

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2020-07-01

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Mishra M, Sharma A, Dagar VS, Kumar S. Effects of β-sitosterol on growth, development and midgut enzymes of Helicoverpa armigera Hübner. Arch Biol Sci [Internet]. 2020Jul.1 [cited 2022Aug.7];72(2):271-8. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5162

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