Glycated ferritin increases the in vitro expression of TLR2 and TLR4 in peripheral blood CD14+ cells obtained from patients with prediabetes



serum ferritin, glycated ferritin, prediabetes, toll-like receptors


Paper description:

  • Glycated ferritin increases the expression of Toll-like receptors (TLR2, TLR4) in CD14+ blood leucocytes derived from healthy subjects. It is not known if a similar response occurs in subjects with prediabetes, a low-grade chronic inflammatory condition.
  • We compared the expression of both receptors in PBMC CD14+ cells from prediabetic and normoglycemic subjects.
  • Glycated ferritin increased TLR2, TLR4, IL-6 and IL-8 expression in cells from prediabetic subjects more than that from normoglycemic subjects. An increase in basal TLR4 expression was only found in cells from prediabetic subjects. This could be part of low-grade inflammation, which could be increased by glycated ferritin.

Abstract: Serum ferritin is a widely-used marker of inflammation in prediabetes, diabetes and atherosclerosis. In these cases, progressive endothelial damage may involve the participation of toll-like receptors (TLR). The aim of this study was to determine the expression of TLR2 and TLR4 in peripheral blood mononuclear cell (PBMC)-derived CD14+ cells from subjects with prediabetes and with a high level of serum ferritin both at baseline and after in vitro cell stimulation with glycated ferritin. Blood samples were drawn from 22 subjects (13 with prediabetes and 9 with normoglycemia). Serum ferritin levels were measured by ELISA, while the expression of TLR2 and TLR4 in PBMC-derived CD14+ cells was determined by flow cytometry. IL-6 and IL-8 cytokines in PBMC-derived CD14+ supernatants were measured by ELISA. Subjects with prediabetes had a higher baseline expression of TLR4 in PBMC-derived CD14+ cells than was observed in cells from normoglycemic subjects (p<0.05). Glycated ferritin increased the expression of both TLR2 and TLR4 as well as IL-6 and IL-8 in PBMC-derived CD14+ cells from subjects with prediabetes when compared to normoglycemic subjects (p<0.05). We concluded that in prediabetes, the increased basal expression of TLR4 could be part of the low-grade inflammation, which could be increased by glycated ferritin.

Received: March 31, 2020; Revised: May 6, 2020; Accepted: May 23, 2020; Published online: May 28, 2020

How to cite this article: Galván-Moroyoqui JM, Martínez-Soto JM, López-Soto LF, Soto-Guzmán JA, Camacho-Villa AY, Alvarez-Hernandez G, Mata-Pineda AL, Candia-PlataMC. Glycated ferritin increases the in vitro expression of TLR2 and TLR4 in peripheral blood CD14+ cells obtained from patients with prediabetes. Arch Biol Sci. 2020:72(3):305-12.



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Author Biography

Alma Yolanda Camacho-Villa, Departamento de Medicina, Universidad Durango Santander, Hermosillo, Sonora


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How to Cite

Galván-Moroyoqui JM, Martínez-Soto JM, López-Soto LF, Soto-Guzmán JA, Camacho-Villa AY, Alvarez-Hernandez G, Mata-Pineda AL, Candia-Plata M del C. Glycated ferritin increases the in vitro expression of TLR2 and TLR4 in peripheral blood CD14+ cells obtained from patients with prediabetes. Arch Biol Sci [Internet]. 2020Oct.19 [cited 2022Aug.7];72(3):305-12. Available from: