Allicin suppresses human glioblastoma cell growth by inducing cell cycle arrest and apoptosis, and by promoting autophagy
Keywords:allicin, glioblastoma, apoptosis, autophagy, cell cycle arrest
- Glioblastoma is one of the most fatal and aggressive cancers with unmet clinical needs. Studies in various cell models reported that allicin induces proapoptotic anticancer effects, albeit the mechanism remains unclear.
- The current study investigated the mechanisms responsible for the inhibitory effects of allicin on the growth of human glioblastoma cell line (DBTRG-05MG).
- Using cell cycle analysis, transmission electron microscopy and LC3 autophagy detection techniques, we provide first evidence that allicin inhibits DBTRG-05MG cell growth by inducing cell cycle arrest and promoting autophagy.
- Our results suggest that allicin can trigger multiple target pathways to induce cancer cell death.
Abstract: Glioblastoma is the most aggressive cancer that occurs in the brain and spinal cord. In the present study, we investigated the effect of allicin, an organosulfur compound obtained from garlic (Allium sativum), on glioblastoma cell growth. When human glioblastoma DBTRG-05MG cells were incubated with different concentrations of allicin for 24 h, cell growth was suppressed in a dose-dependent manner. The results from image-based cytometer assays suggested that allicin caused S and G2/M phase cell cycle arrest and induced apoptosis. Autophagy detection studies showed that allicin also promoted this mechanism. Because cell migration is a key process during tumor formation, the effect of allicin on glioblastoma cell migration was also examined. After allicin treatment, the migration ability of cells decreased when compared with the control after 24 h. Taken together, the present results suggested that allicin inhibited human glioblastoma cell growth by inducing S and G2/M phase cell cycle arrest, apoptosis and autophagy. Our findings suggest that allicin suppressed glioblastoma cell growth through multiple target pathways. Therefore, allicin potentially serves as an alternative therapeutic candidate or could be synergistically used in combination with the standard drug for the treatment of glioblastoma multiforme.
Received: April 14, 2020; Revised: May 17, 2020; Accepted: May 27, 2020; Published online: June 2, 2020
How to cite this article: Weeranantanapan O, Satsantitham K, Sritangos P, Chudapongse N. Allicin suppresses human glioblastoma cell growth by inducing cell cycle arrest and apoptosis, and by promoting autophagy. Arch Biol Sci. 2020;72(3):313-9.
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