Modulation of TNF-α plasma levels in coronary artery disease [CAD] and non-CAD male patients by lncRNA UCA1 and aspirin

Authors

  • Peyman Nowrouzi-Sohrabi 1. Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz; 2. Student Research Committee, Shiraz University of Medical Sciences, Shiraz https://orcid.org/0000-0002-9720-2025
  • Atefeh Seghatoleslam 1. Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz; 2. Research Center for Traditional Medicine and History of Medicine, Medical School, Shiraz University of Medical Sciences, Shiraz https://orcid.org/0000-0002-8277-6608
  • Peyman Izadpanah Cardiology Department, Shiraz University of Medical sciences, Shiraz https://orcid.org/0000-0002-9479-2015
  • Mehran Erfani Department of Medical Laboratory Sciences, Faculty of Medical Sciences, Islamic Azad University, Arak Branch, Arak https://orcid.org/0000-0001-9593-5383
  • Hassan Ahmadvand 1. Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad; 2. Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khorramabad https://orcid.org/0000-0002-9406-3592
  • Mehdi Kalani Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz https://orcid.org/0000-0002-7091-6466

DOI:

https://doi.org/10.2298/ABS200616040N

Keywords:

aspirin, noncoding RNA, UCA1, TNF-α, coronary artery disease

Abstract

Paper description:

  • The expression of long non-coding RNA urothelial carcinoma-associated 1 (UCA1) expression and its association with IL-6, IL-22 and TNF-α in patients with coronary artery disease (CAD) and healthy non-CAD (NCAD) individuals was examined.
  • UCA1 expression in peripheral blood mononuclear cells (PBMCs) and IL-6, IL-22 and TNF-α plasma levels in CAD and NCAD groups were assessed by real-time PCR and flowcytometry, respectively.
  • UCA1 expression was not significantly different between the groups. Its higher level in aspirin users was indirectly associated with a decrease in plasma TNF-α.
  • UCA1 upregulation might be an underlying mechanism for downregulation of TNF-α in aspirin users.

Abstract: The aim of this study was to investigate the expression of long non-coding RNA urothelial carcinoma-associated 1 (UCA1) and its role in TNF-α production as one of the main inflammatory cytokines, in peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD) and healthy non-CAD (NCAD) individuals as the control group. Fifteen CAD and 15 NCAD individuals were enrolled in the study. UCA1 expression in PBMCs and the plasma concentrations of interleukin (IL)-6, IL-22 and tumor necrosis factor (TNF)-α were assessed by real-time PCR and flowcytometry, respectively. UCA1 expression was not significantly different between the CAD and NCAD groups, however, its level was higher in PBMCs of regular aspirin users of both study groups. Furthermore, aspirin users showed a significantly lower plasma level of TNF-α in comparison to non-aspirin users. In addition, UCA1 expression was negatively correlated with the level of TNF-α in the total sample of the examined population. It seems that the increased levels of UCA1 may be an underlying mechanism for downregulation of TNF-α in aspirin users.

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References

Camero Y. Management of Coronary Artery Disease and Chronic Stable Angina. US Pharm. 2017;42(2):27-31.

Mensah GA, Wei GS, Sorlie PD, Fine LJ, Rosenberg Y, Kaufmann PG, Mussolino, Michael E, Hsu LL, Addou E, Engelgau MM. Decline in cardiovascular mortality: possible causes and implications. Circ Res. 2017;120(2):366-80.

Ford ES, Ajani UA, Croft JB, Critchley JA, Labarthe DR, Kottke TE, Giles WH, Capewell S. Explaining the decrease in US deaths from coronary disease, 1980–2000. N Engl J Med. 2007;356(23):2388-98.

Mora S, Manson JE. Aspirin for primary prevention of atherosclerotic cardiovascular disease: advances in diagnosis and treatment. JAMA Intern Med. 2016;176(8):1195-204.

Unal B, Critchley JA, Capewell S. Explaining the decline in coronary heart disease mortality in England and Wales between 1981 and 2000. Circulation. 2004;109(9):1101-7.

Moreira DM, da Silva RL, Vieira JL, Fattah T, Lueneberg ME, Gottschall CA. Role of vascular inflammation in coronary artery disease: potential of anti-inflammatory drugs in the prevention of atherothrombosis. Inflammation and anti-inflammatory drugs in coronary artery disease. Am J Cardiovasc Drugs. 2015;15(1):1-11.

Bibbins-Domingo K. Aspirin use for the primary prevention of cardiovascular disease and colorectal cancer: US Preventive Services Task Force recommendation statement. Ann Intern Med. 2016;164(12):836-45.

Antithrombotic Trialists' Collaboration. Collaborative meta-analysis of randomised trials of antiplatelet therapy for prevention of death, myocardial infarction, and stroke in high risk patients. Bmj. 2002;324(7329):71-86.

Schonrock N, Harvey RP, Mattick JS. Long noncoding RNAs in cardiac development and pathophysiology. Circ Res. 2012;111(10):1349-62.

An J, Chen Z, Ma Q, Wang H, Zhang J, Shi F. LncRNA SNHG16 promoted proliferation and inflammatory response of macrophages through miR-17-5p/NF-κB signaling pathway in patients with atherosclerosis. Eur Rev Med Pharmacol Sci. 2019;23(19):8665-77.

Zhang BY, Jin Z, Zhao Z. Long intergenic noncoding RNA 00305 sponges miR-136 to regulate the hypoxia induced apoptosis of vascular endothelial cells. Biomed Pharmacother. 2017;94:238-43.

Holdt LM, Hoffmann S, Sass K, Langenberger D, Scholz M, Krohn K, Finstermeier K, Stahringer A, Wilfert W, Beutner F. Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks. PLoS Genet. 2013;9(7).

Michalik KM, You X, Manavski Y, Doddaballapur A, Zörnig M, Braun T, John D, Ponomareva Y, Chen W, Uchida S. Long noncoding RNA MALAT1 regulates endothelial cell function and vessel growth. Circ Res. 2014;114(9):1389-97.

Huang Y, Wang L, Mao Y, Nan G. Long noncoding RNA-H19 contributes to atherosclerosis and induces ischemic stroke via the upregulation of acid phosphatase 5. Front Neurol. 2019;10:32.

Wu G, Cai J, Han Y, Chen J, Huang Z-P, Chen C, Cai Y, Huang H, Yang Y, Liu Y. LincRNA-p21 regulates neointima formation, vascular smooth muscle cell proliferation, apoptosis, and atherosclerosis by enhancing p53 activity. Circulation. 2014;130(17):1452-65.

Liu Y, Zhou D, Li G, Ming X, feng Tu Y, Tian J, Lu H, Yu B. Long non coding RNA-UCA1 contributes to cardiomyocyte apoptosis by suppression of p27 expression. Cell Physiol Biochem. 2015;35(5):1986-98.

Zhou G, Li C, Feng J, Zhang J, Fang Y. lncRNA UCA1 is a novel regulator in cardiomyocyte hypertrophy through targeting the miR-184/HOXA9 axis. Cardiorenal Med. 2018;8(2):130-9.

Yu X, Zou T, Zou L, Jin J, Xiao F, Yang J. Plasma long noncoding RNA urothelial carcinoma associated 1 predicts poor prognosis in chronic heart failure patients. Med Sci Monit. 2017;23:2226.

Wang CJ, Zhu CC, Xu J, Wang M, Zhao WY, Liu Q, Zhao G, Zhang ZZ. The lncRNA UCA1 promotes proliferation, migration, immune escape and inhibits apoptosis in gastric cancer by sponging anti-tumor miRNAs. Mol Cancer. 2019;18(1):115.

Guo H, Liu J, Ben Q, Qu Y, Li M, Wang Y, Chen W, Zhang J. The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation. Genome Biol. 2016;17(1):24.

Zhen S, Lu J, Chen W, Zhao L, Li X. Synergistic Antitumor Effect on Bladder Cancer by Rational Combination of Programmed Cell Death 1 Blockade and CRISPR-Cas9-Mediated Long Non-Coding RNA Urothelial Carcinoma Associated 1 Knockout. Hum Gene Ther. 2018;29(12):1352-63.

Peng F, Wang J-H, Fan W-J, Meng Y-T, Li M-M, Li T-T, Cui B, Wang H-F, Zhao Y, An F. Glycolysis gatekeeper PDK1 reprograms breast cancer stem cells under hypoxia. Oncogene. 2018;37(8):1062-74.

Yao F, Wang Q, Wu Q. The prognostic value and mechanisms of lncRNA UCA1 in human cancer. Cancer Manag Res. 2019;11:7685-96.

Wang QS, Zhou J, Li X. LncRNA UCA1 protects cardiomyocytes against hypoxia/reoxygenation induced apoptosis through inhibiting miR-143/MDM2/p53 axis. Genomics. 2020;112(1):574-80.

Chen J, Hu Q, Zhang BF, Liu XP, Yang S, Jiang H. Long noncoding RNA UCA1 inhibits ischaemia/reperfusion injury induced cardiomyocytes apoptosis via suppression of endoplasmic reticulum stress. Genes Genomics. 2019;41(7):803-10.

Yu Q, Zhao MW, Yang P. LncRNA UCA1 Suppresses the Inflammation Via Modulating miR-203-Mediated Regulation of MEF2C/NF-kappaB Signaling Pathway in Epilepsy. Neurochem Res. 2020;45(4):783-95.

Cai L, Tu L, Li T, Yang X, Ren Y, Gu R, Zhang Q, Yao H, Qu X, Wang Q, Tian, J. Downregulation of lncRNA UCA1 ameliorates the damage of dopaminergic neurons, reduces oxidative stress and inflammation in Parkinson's disease through the inhibition of the PI3K/Akt signaling pathway. Int Immunopharmacol. 2019;75:105734.

Wang TP. Association between TNF-α polymorphisms and the risk of upper gastrointestinal bleeding induced by aspirin in patients with coronary heart disease. Ann Hum Genet. 2019;83(3):124-33.

Guo H, Liu J, Ben Q, Qu Y, Li M, Wang Y, Chen W, Zhang J. The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation. Genome Biol. 2016;17:24.

Hohlfeld T, Schrör K. Antiinflammatory effects of aspirin in ACS: relevant to its cardiocoronary actions? Thromb Haemost. 2015;114(3):469-77.

Liu Y, Fang S, Li X, Feng J, Du J, Guo L, Su Y, Zhou J, Ding G, Bai, Y, Wang S, Wang H, Liu Y. Aspirin inhibits LPS-induced macrophage activation via the NF-kappaB pathway. Sci Rep. 2017;7(1):11549.

Yang JM, Rui BB, Chen C, Chen H, Xu TJ, Xu WP, Wei W. Acetylsalicylic acid enhances the anti-inflammatory effect of fluoxetine through inhibition of NF-kappaB, p38-MAPK and ERK1/2 activation in lipopolysaccharide-induced BV-2 microglia cells. Neuroscience. 2014;275:296-304.

Zhang T, Xiu HH, Liu JX, Ma Y, Xu KQ, Huang WQ. Protective effect of aspirin-triggered resolvin D1 on hepatic ischemia/reperfusion injury in rats: The role of miR-146b. Int Immunopharmacol. 2017;51:140-7.

Hasan F, Ikram R, Simjee SU, Iftakhar K, Asadullah K, Usman M. The effects of aspirin gel and mouthwash on levels of salivary biomarkers PGE2, TNF-alpha and nitric oxide in patients with periodontal diseases. Pak J Pharm Sci. 2019;32(5):2019-23.

Slomiany BL, Slomiany A. Aspirin ingestion impairs oral mucosal ulcer healing by inducing membrane-bound tumor necrosis factor-alpha release. IUBMB life. 2000;50(6):391-5.

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Published

2020-12-25

How to Cite

1.
Nowrouzi-Sohrabi P, Seghatoleslam A, Izadpanah P, Erfani M, Ahmadvand H, Kalani M. Modulation of TNF-α plasma levels in coronary artery disease [CAD] and non-CAD male patients by lncRNA UCA1 and aspirin. Arch Biol Sci [Internet]. 2020Dec.25 [cited 2022Aug.7];72(4):465-71. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6033

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