Age-related changes of choroid plexus morphology, vascularization and epithelial proliferation

Authors

  • Golub D. Samardzija Department of Pathology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad, Serbia
  • Bojana M. Andrejic-Visnjic Department of Histology and Embryology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova, Novi Sad, Serbia
  • Matilda A. Djolai Department of Histology and Embryology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova, Novi Sad, Serbia
  • Jelena R. Ilic-Sabo Department of Histology and Embryology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova, Novi Sad, Serbia
  • Jelena P. Amidzic Department of Histology and Embryology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova, Novi Sad, Serbia
  • Zoran Nikin Department of Pathology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad, Serbia
  • Natali Z. Rakocevic Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad, Serbia
  • Nikola N. Aleksic Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad, Serbia
  • Borislav E. Dolamic Department for Specialistic-Consulting Work, Health Center Novi Sad, Bulevar cara Lazara 75, Novi Sad, Serbia
  • Biserka B. Vukomanovic-Djurdjevic Department of Histology, Military Medical Academy, University of Defence, Crnotravska 17, 11000 Belgrade, Serbia

DOI:

https://doi.org/10.2298/ABS201210014S

Keywords:

choroid plexus, ageing, psammoma body, amyloid, vascularization

Abstract

Paper description:

  • Dementia is thought to begin with changes in the blood-cerebrospinal fluid barrier, which makes ageing-associated alterations of the choroid plexus (ChP) a compelling topic.
  • This study provides a histomorphometric analysis of psammoma bodies (PBs), vasculature, amyloid deposition, and epithelium of the ChP among young, middle-aged and older individuals.
  • Ageing was associated with an increase in the number of PBs (specifically immature forms), and a significant decrease in the vascular area, accompanied by amyloid deposition.
  • Marked age-related changes provide a unique basis for comparison with pathological changes. Given the increase in immature PBs in the elderly, we challenge the given terminology.

Abstract: The choroid plexus (ChP) is essential for brain homeostasis by regulating the secretion of the cerebrospinal fluid (CSF). Despite a substantial body of work on the pathologically alterations of the ChP, there is lack of data concerning the naturally occurring morphological changes asso-ciated with ageing. In this study, we investigated 30 human ChP tissue samples that were divided into 3 groups according to age. Morphometric analysis of psammoma bodies (PBs) on hematoxylin and eosin (H&E)-stained samples and immunohistochemical analysis of ChP were performed (using antibodies to transmembrane phosphoglycoprotein protein CD34 and nuclear protein Ki-67). Amyloid deposits were detected using Congo red staining. Middle-aged and older individuals exhibited a significantly higher numerical density (ndPB) mostly as increased immature forms, which led us to question the proposed nomenclature. The proliferation rate of the ChP epithelium did not show significant difference between groups. The vascular area was markedly decreased and accompanied by amyloid deposition in blood vessel walls. While the deposits were limited to middle-sized blood vessels in the middle-aged group, in the older group deposits were also present around small vessels. The identified major morphological alterations of ageing ChP tissue provide further understanding of disfunctions of the blood-cerebrospinal fluid barrier that underlie neurodegenerative disorders.

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Published

2021-07-13

How to Cite

1.
Samardzija GD, Andrejic-Visnjic BM, Djolai MA, Ilic-Sabo JR, Amidzic JP, Nikin Z, Rakocevic NZ, Aleksic NN, Dolamic BE, Vukomanovic-Djurdjevic BB. Age-related changes of choroid plexus morphology, vascularization and epithelial proliferation. Arch Biol Sci [Internet]. 2021Jul.13 [cited 2022Aug.7];73(2):197-204. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6043

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