Altered diversity of bacterial communities in two Drosophila species under laboratory conditions and lead exposure

Authors

  • Mirjana B. Beribaka Faculty of Technology, University of East Sarajevo, Karakaj 34A, 75400 Zvornik
  • Ivica Z. Dimkić Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade
  • Mihailo Đ. Jelić Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade
  • Slaviša M. Stanković Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade
  • Novo M. Pržulj Faculty of Agriculture, University of Banja Luka, Bulevar vojvode Petra Bojovića 1A, 78000 Banja Luka
  • Marko Lj. Anđelković Serbian Academy of Sciences and Arts, Kneza Mihaila 35, 11000 Belgrade
  • Marina M. Stamenković-Radak Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade

DOI:

https://doi.org/10.2298/ABS200911054B

Keywords:

Drosophila melanogaster, Drosophila subobscura, lead exposure, microbiota diversity, next-generation sequencing

Abstract

Paper description:

  • Changes in climatic and environmental parameters give relevance to the study of factors that shape the interactions between insects and microbiota, and to their adaptive significance.
  • Microbiota diversity in two Drosophila species from field-collected samples and their laboratory progeny with and without exposure to lead pollution is the first investigation of the bacterial communities associated with subobscura.
  • Significant species-specific differences in bacterial composition between populations and treatments was revealed. The diversity of bacterial communities increased in both species under lead exposure.
  • Increased microbiota diversity under lead pollution could serve as an indicator of the adaptive response to environmental stress.

Abstract: The composition of microbiota affects different traits of Drosophila throughout its life cycle and represents an important part of the adaptive response to environmental changes, such as increased levels of accumulated heavy metals in their habitats. We investigated the effects of lead added to the feeding substrate on the microbiota diversity in the fruit flies, Drosophila melanogaster and Drosophila subobscura. We compared the bacterial diversity of wild-caught flies and their progeny reared under standard laboratory conditions. We analyzed the shifts in bacterial diversity in the experimental groups of flies reared for 13 generations on standard and lead(II) acetate (Pb(CH3COO)2), Pb acetate-saturated substrates. Identification of the main bacterial genera was performed by next-generation sequencing (NGS) of the V3-V4 variable regions of the 16S rRNA gene. Results indicate significant species-specific differences in bacterial composition between natural and laboratory populations, and between the substrates. Diversity increased in both species under prolonged exposure to lead-polluted substrate, suggesting a potential adaptive response to an environmental stress.

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Published

2021-03-19

How to Cite

1.
Beribaka MB, Dimkić IZ, Jelić M Đ., Stanković SM, Pržulj NM, Anđelković ML, Stamenković-Radak MM. Altered diversity of bacterial communities in two Drosophila species under laboratory conditions and lead exposure. Arch Biol Sci [Internet]. 2021Mar.19 [cited 2022Aug.7];73(1):17-29. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6320

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