Evaluation of two different dendritic cell preparations to BCG reactivity

Authors

  • Marek Fol Department of Immunology and Infectious Biology, Institute for Microbiology, Biotechnology and Immunology, University of Lodz, 90-237 Lodz
  • Anna Nitecka-Blaźlak Department of Immunology and Infectious Biology, Institute for Microbiology, Biotechnology and Immunology, University of Lodz, 90-237 Lodz
  • Piotr Szpakowski Department of Immunology and Infectious Biology, Institute for Microbiology, Biotechnology and Immunology, University of Lodz, 90-237 Lodz
  • Murty V.V.S. Madiraju University of Texas Health Science Center at Tyler, Tyler TX 75708
  • Wiesława Rudnicka Department of Immunology and Infectious Biology, Institute for Microbiology, Biotechnology and Immunology, University of Lodz, 90-237 Lodz
  • Magdalena Druszczyńska Department of Immunology and Infectious Biology, Institute for Microbiology, Biotechnology and Immunology, University of Lodz, 90-237 Lodz
  • Joël Pestel CNRS-UMR 8576, Unité de Glycobiologie Structurale et Fonctionnelle, IFR 147, Université Lille Nord de France, 59655 Villeneuve d’Ascq
  • Magdalena Kowalewicz-Kulbat Department of Immunology and Infectious Biology, Institute for Microbiology, Biotechnology and Immunology, University of Lodz, 90-237 Lodz

Abstract

Dendritic cells (DCs) play a key-role in the immune response against intracellular bacterial pathogens, including mycobacteria. Monocyte-derived dendritic cells (MoDCs) are considered to behave as inflammatory cell populations. Different immunomagnetic methods (positive and negative) can be used to purify monocytes before their in vitro differentiation and their culture behavior can be expected to be different. In this study we evaluated the reactivity of two dendritic cell populations towards the Bacillus Calmette–Guérin (BCG) antigen. Monocytes were obtained from the blood of healthy donors, using positive and negative immunomagnetic separation methods. The expression of DC-SIGN, CD86, CD80, HLA-DR and CD40 on MoDCs was estimated by flow cytometry. The level of IL-12p70, IL-10 and TNF-α was measured by ELISA. Neither of the tested methods affected the surface marker expression of DCs. No significant alteration in immunological response, measured by cytokine production, was noted either. After BCG stimulation, the absence of IL-12, but the IL-23 production was observed in both cell preparations. Positive and negative magnetic separation methods are effective techniques to optimize the preparation of monocytes as the source of MoDCs for potential clinical application

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Published

2016-06-24

How to Cite

1.
Fol M, Nitecka-Blaźlak A, Szpakowski P, Madiraju MV, Rudnicka W, Druszczyńska M, Pestel J, Kowalewicz-Kulbat M. Evaluation of two different dendritic cell preparations to BCG reactivity. Arch Biol Sci [Internet]. 2016Jun.24 [cited 2024May23];68(2):263-71. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/765

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