The effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in Capsicum annuum L.: A comparative study of extraction methods and solvents

Authors

DOI:

https://doi.org/10.2298/ABS230601027C

Keywords:

Mycorrhiza, Capsaicin, Ultrasound-assisted extraction, Funneliformis mosseae, Capsicum annuum, gas chromatography-mass spectrometry (GC-MS)

Abstract

Paper description:

  • The aim of this study was to determine the effect of arbuscular mycorrhizal fungi on capsaicinoid production in Capsicum annuum, along with alterations in physiological and biochemical properties.
  • Magnetic stirring extraction and ultrasound-assisted extraction using three different solvents were used to determine capsaicinoids by GC-MS.
  • The highest capsaicin content up to 4-fold was found in plants inoculated with Funneliformis mosseae using ultrasound-assisted extraction with ethyl acetate. Plants with a mycorrhizal partner exhibited better physiological and biochemical properties.
  • Mycorrhizal fungi contribute to plant growth and development and enhance the production of valuable secondary metabolites in plants.

Abstract: This study aimed to determine the effect of two mycorrhizal fungi, Funneliformis mosseae (Fm) and Rhizophagus intraradices (Ri), on capsaicinoid production in Capsicum annuum L. by gas chromatography-mass spectrometry (GC-MS) via two different extraction approaches, magnetic stirring and ultrasound-assisted extraction with three different solvents, ethanol (EtOH), ethyl acetate (EtAce), and acetonitrile (AceN). The effect of mycorrhizal fungi on some physiological properties and biochemical activity, the content of total phenolic compounds, and antioxidant activity were also investigated. For all investigated parameters, the plants inoculated with mycorrhizal fungi showed significantly higher values than the non-mycorrhizal control plants, except for malondialdehyde (MDA), which was an indicator of lipid peroxidation due to damage that occurred in the cell membrane. It was concluded that inoculation with mycorrhizal fungi increased both capsaicin and dihydrocapsaicin production up to 4-fold in C. annuum compared to the control. Results also indicated that ultrasound-assisted extraction with EtAce was the most effective method for the determination of capsaicin by GC-MS.

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2023-10-26

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Canpolat Şükrü, İşlek C. The effect of arbuscular mycorrhiza on physiological and biochemical parameters and capsaicinoid production in Capsicum annuum L.: A comparative study of extraction methods and solvents. Arch Biol Sci [Internet]. 2023Oct.26 [cited 2024Feb.29];75(3):327-39. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/8756

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