Assessment of antioxidant and antiinflammatory activities and acute toxicity of the aqueous extract from a mixture of leaves and flowers of Anabasis articulata (Forssk.) Moq.

Authors

DOI:

https://doi.org/10.2298/ABS240122011M

Keywords:

Anabasis articulata (Forssk.) Moq., polyphenols, antioxidants, acute toxicity, anti-inflammatory activity

Abstract

Paper description:

  • Anabasis articulata (Forssk) Moq. was used in traditional medicine to treat fever, headaches, diabetes, and skin disorders including eczema and lice.
  • Quantification of secondary metabolites, examination of antioxidant activity, and acute toxicity of the aqueous extract from mixtures of articulata flowers and leaves, and the in vitro and in vivo anti-inflammatory activity of the aqueous extract was examined.
  • The extract exhibited in vitro antioxidant activity and showed in vitro and in vivo anti-inflammatory activities in different models.
  • This plant has potential beneficial effects for treating diseases associated with inflammation.

Abstract: Colometric assays were used to quantify the secondary metabolites obtained by a decoction of the extract of Anabasis articulata (DEAA) flowers and leaves. Antioxidant activity was examined using several methods: total antioxidant capacity, the 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging assay, and the β-carotene bleaching assay. Single oral doses of 2000 and 5000 mg/kg body weight were administered to albino mice to assess acute toxicity. In vitro antiinflammatory activity was determined using the egg albumin denaturation test, and the in vivo inflammatory effect was assessed utilizing carrageenan, croton oil, and xylene-induced edema tests. Results showed that DEAA contained high amounts of polyphenols, flavonoids, and tannins and exhibited antioxidant activity in all tested assays. DEAA inhibited protein denaturation and did not cause any mortality or adverse effects. Oral administration of 200 mg/kg DEAA significantly reduced the edema induced by carrageenan, croton oil, and xylene. This study highlights the use of Anabasis articulata (Forssk.) Moq. in traditional herbal medicine. It possesses antioxidant activity and can be considered safe for oral consumption; it also has potential beneficial effects in treating diseases associated with inflammation and pain.

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References

Abdallaha HM, Abdel-Naimc AB, Ashourd OM, Shehataa IA, Abdel-Sattarb EA. Anti-inflammatory activity of selected plants from Saudi Arabia. Z Naturforsch C J Biosci. 2014;69(1-2):1-9. https://doi.org/10.5560/ZNC.2012-0168

Okoli CO, Akah PA, Nwafor SV. Anti-inflammatory activity of plants. J Nat Remedies. 2003;3(1):1-30.

Gong C, Hoff JT, Keep RF. Acute inflammatory reaction following experimental intracerebral hemorrhage in rat. Brain Res. 2000;871(1):57-65. https://doi.org/10.1016/S0006-8993(00)02427-6

Li RW, Myers SP, Leach DN, Lin GD, Leach G. A cross-cultural study: anti-inflammatory activity of Australian and Chinese plants. J Ethnopharmacol. 2003;85(1):25-32. https://doi.org/10.1016/S0378-8741(02)00336-7

Calixto JB, Beirith A, Ferreira J, Santos ARS, Filho C, Yunes RA. Naturally occurring antinociceptive substances from plants. Phytother Res. 2000;14(6):401-18.

Hammiche V, Maiza K. Traditional medicine in Central Sahara: Pharmacopoeia of TassiliN’ajjer. J Ethnopharmacol. 2006;105(3):358-67. https://doi.org/10.1016/j.jep.2005.11.028

Khafagi A, Marei A, Mohamed S. Morphological and anatomical variations of Anabasis articulata ecotypes in Egypt. Arab Univ J AgricSci. 2005;13(2):233-48. https://doi.org/10.21608/ajs.2005.15350

Kambouche N, Merah B, Derdour A, Bellahouel S, Bouayed J, Dicko A, Younos C, Soulimani R. Hypoglycemic and antihyperglycemic effects of Anabasis articulata (Forssk) Moq (Chenopodiaceae), an Algerian medicinal plant. Afr J Biotechnol. 2009;8(20):5589-94.

Bouaziz M, Dhouib A, Loukil S, Boukhris M, Sayadi S. Polyphenols content, antioxidant and antimicrobial activities of extracts of some wild plants collected from the south of Tunisia. Afr J Biotechnol. 2009;8(24):7017-27.

Abdulsahib WK, Abd AH, Qasim BJ, Sahib HB. Antiangiogenesis and antioxidant effect of Anabasis articulata stems extracts. Int J Pharm Sci Rev Res. 2016;41(2):88-94.

Abdulhusain ZH, Mahdi MA, Abdulsahib WK, Jasim LS. Anabasis articulata exerts an anti-arthritic effect on adjuvant-induced arthritis in rats. J Adv Pharm Technol Res. 2022;13(4):276-80.

Metwally NS, Mohamed AM, ELSharabasy FS. Chemical constituents of the Egyptian plant Anabasis articulata (Forssk) Moq and its antidiabetic effects on rats with streptozotocin-induced diabetic hepatopathy. J Appl Pharm Sci. 2012;2(4):54-65. https://doi.org/10.7324/JAPS.2012.2403

Djeridane A, Hamdi A, Bensania W, Cheifa K, Lakhdari I, Yousfi M. The in vitro evaluation of antioxidative activity, α-glucosidase and α-amylase enzyme inhibitory of natural phenolic extracts. Diabetes Metab Syndr. 2015;9(4):324-31. https://doi.org/10.1016/j.dsx.2013.10.007

Senhaji S, Lamchouri F, Toufik H. Phytochemical Content, Antibacterial and Antioxidant Potential of Endemic Plant Anabasis aretioïdesCoss. &Moq. (Chenopodiaceae). Biomed Res. Int. 2020;2020:1-16. https://doi.org/10.1155/2020/6152932

Dehimi K, Djoudi Z, Boulaouad A, Maadadi AR, Khennouf S. A Contribution to the Valorization of Two Medicinal Plants: Atriplex Halimus Sub. Sp. Schweinfurthii and BuniumIncrassatum, Growing in the Region of M’sila (North-East Algeria). IJNDD. 2020;12(4):208-16.

Gamal G, Abouelsaoud K, Elekhnawy E, Attia G. Anti-Biofilm Impact of Anabasis articulata (Forssk) Moq. Total Methanol Extract Against Pseudomonas aeruginosa Clinical Isolates. JAMPR. 2022;3(1):29-35. https://doi.org/10.21608/jampr.2022.131451.1027

Belyagoubi-Benhammou N, Belyagoubi L, Gismondi A, Di Marco G, Canini A, Atik Bekkara F. GC/MS analysis, and antioxidant and antimicrobial activities of alkaloids extracted by polar and apolar solvents from the stems of Anabasis articulata. Med Chem Res. 2019;28:754-767. https://doi.org/10.1007/s00044-019-02332-6

Ferreira A, Proença C, Serralheiro MLM, Araújo MEM. The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. J Ethnopharmacol. 2006;108(1):31-7. https://doi.org/10.1016/j.jep.2006.04.010

Li H, Cheng K, Wong C, Fan K, Chen F, Jiang Y. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chem. 2007;102(3):771-6. https://doi.org/10.1016/j.foodchem.2006.06.022

Bahorun T, Gressier B, Trotin F, Brunet C, Dine T, Luyckx M, Vasseur J, Cazin M, Cazin JC, Pinkas M. Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittelforschung. 1996;46(11):1086-9.

Prasanth DSNBK, Rao AS, Yejella RP. Phytochemical, in vitro antioxidant and antibacterial activities of Argyreiapilosa wight &arn. (whole plant). Int J Pharmacogn. 2017;4(4):109-17. https://doi.org/10.13040/IJPSR.0975-8232.IJP.4(4).109-117

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E.Anal Biochem. 1999;269(2):337-41.

Shimamura T, Sumikura Y, Yamazaki T, Tada A, Kashiwagi T, Ishikawa H, Matsui T, Sugimoto N, Akiyama H, Ukeda H. Applicability of the DPPH assay for evaluating the antioxidant capacity of food additives-inter-laboratory evaluation study. Anal Sci. 2014;30(7):717-21. https://doi.org/10.2116/analsci.30.717

Kartal N, Sokmen M, Tepe B, Daferera D, Polissiou M, Sokmen A. Investigation of the antioxidant properties of Ferulaorientalis L. using a suitable extraction procedure. Food Chem. 2007;100(2):584-9. https://doi.org/10.1016/j.foodchem.2005.09.084

Bouaziz A, Djidel S, Bentaher A, Khennouf S. Polyphenolic content, Antioxidant and Anti-inflammatory activities of Melon (Cucumis melo L. var. inodorus) Seeds. JDDT. 2020;10(2-s):22-6.

OECD O. Guidelines for testing of chemicals, acute oral toxicity-fixed dose procedure. Published online 2001.

Tsai CC, Lin CC. Anti-inflammatory effects of Taiwan folk medicine ‘Teng-Khia-U’ on carrageenan- and adjuvant-induced paw edema in rats. J Ethnopharmacol. 1998;64(1):85-9. https://doi.org/10.1016/S0378-8741(98)00108-1

Dulcetti Junior O, Andreucci VC, Cunha IB da S, Araujo CEP, de Oliveira F, Marcucci MC. Investigation of the anti-inflammatory and analgesic activities of a sample of Brazilian propolis. Acta Farm Bonaerense. 2004;23(3):285-91.

Manouze H, Bouchatta O, Gadhi AC, Bennis M, Sokar Z, Ba-M’hamed S. Anti-inflammatory, antinociceptive, and antioxidant activities of methanol and aqueous extracts of Anacyclus pyrethrum roots. Front pharmacol. 2017;8:598. https://doi.org/10.3389/fphar.2017.00598

Benhammou N, Bekkara FA, Panovska TK. Antioxidant activity of methanolic extracts and some bioactive compounds of Atriplex halimus. C R Chim. 2009;12(12):1259-66.

Markom M, Hasan M, Daud W, Singh H, Jahim J. Extraction of hydrolysable tannins from Phyllanthus niruriLinn.: Effects of solvents and extraction methods. Sep Purif Technol. 2007;52(3):487-96. https://doi.org/10.1016/j.seppur.2006.06.003

Wijngaard H, Hossain MB, Rai DK, Brunton N. Techniques to extract bioactive compounds from food by-products of plant origin. Food Res Int. 2012;46(2):505-13. https://doi.org/10.1016/j.foodres.2011.09.027

Benhammou N, Ghambaza N, Benabdelkader S, Atik-Bekkara F, Panovska FK. Phytochemicals and antioxidant properties of extracts from the root and stems of Anabasis articulata. Int Food Res J. 2013;20(5):2057-63.

El-Haci IA, Bekkara FA, Mazari W, Gherib M. Phenolics content and antioxidant activity of some organic extracts of endemic medicinal plant Anabasis aretioides Coss. &Moq. from Algerian Sahara. Pharmacogn J. 2013;5(3):108-12. https://doi.org/10.1016/j.phcgj.2013.05.004

Berrani A, Marmouzia I, Kharbach M, Bouyahya A, El Hamdani M, El Jemli M, Lrhorfi A, Benassaoui H, Zouarhi M, Larbi OM, El Abbes Faouzi M, Bengueddour R. Anabasis aretioidesCoss. &Moq. phenolic compounds exhibit in vitro hypoglycemic, antioxidant and antipathogenic properties. JBCPP. 2019;30(2):251-7. https://doi.org/10.1515/jbcpp-2018-0154

Dudonne S, Vitrac X, Coutiere P, Woillez M, Mérillon JM. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J Agric Food Chem. 2009;57(5):1768-74. https://doi.org/10.1021/jf803011r

Bouaziz A, KhennoufS, ZargaMA, Abdalla S, Baghiani A, Charef N. Phytochemical analysis, hypotensive effect and antioxidant properties of Myrtus communis L. growing in Algeria. Asian Pac J Trop Biomed. 2015;5(1):19-28. https://doi.org/10.1016/S2221-1691(15)30165-9

Mohammed H A, Alshalmani SK, Abdellatif AG.Antioxidant and quantitative estimation of phenolic and flavonoids of three halophytic plants growing in Libya. J Pharmacog Photochem. 2013;2(3):89-94.

Kamath V, Rajini PS. The efficacy of cashew nut (Anacardium occidentale L.) skin extract as a free radical scavenger. Food Chem. 2007;103(2):428-33. https://doi.org/10.1016/j.foodchem.2006.07.031

Solomon A, Golubowicz S, Yablowicz Z, Grossman S, Bergman M, Gottlieb HE, Altman A, Kerem Z, Flaishman MA. Antioxidant activities and anthocyanin content of Fresh Fruits of Common Fig (Ficus caricaL.). J Agric Food Chem. 2006;54(20):7717-23. https://doi.org/10.1021/jf060497h

Li HB, Wong CC, Cheng KW, Chen F. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT - Food Sci Technol. 2008;41(3):385-90. https://doi.org/10.1016/j.lwt.2007.03.011

Kiranmayi GVN, Anusha V, Chandrika Y, Priya IVS. Preliminary phytochemical screening and in vitro evaluation of anti-inflammatory, antiarthritic, and thrombolytic activities of ethanolic leaf extract of Bauhinia purpurea. Int J Green Pharm. 2018;12(1):S241-7.

De P, Sarkar S, Mukhophadhyay MJ. Anti protein denaturation activity and bioactive compound screening of Piper betel aqueous and alcoholic leaf extract. J pharmacognphytochem. 2017;6(2):52-5.

Henneh IT, Ameyaw EO, Biney RP, Armah FA, Obese E, Konjah D, Teye E.Ziziphusabyssinica hydro-ethanolic root bark extract attenuates acuteinflammation possibly through membrane stabilization and inhibition of proteindenaturation and neutrophil degranulation. West Afr Pharm. 2018;29(2):81-94.

Mahdjar S, Bakka C, Dendougui H, Hadjadj M. Phytochemical profile and in vitro anti-inflammatory activity of Anvillea radiata (Coss and Dur) flowers extracts. AJRC. 2020;13(1):44. https://doi.org/10.5958/0974-4150.2020.00010.3

Guefack MGF, Damen F, Ngaffo CMN, Kuete V. Acute and sub-chronic toxicities assessment of methanol bark extract of Hypericum roeperianum in rats. S Afr J Bot. 2022;150:691-701. https://doi.org/10.1016/j.sajb.2022.08.006

George P. Concerns regarding the safety and toxicity of medicinal plants - An overview. J Appl Pharm Sci. 2011;1(6):40-4.

Sireeratawong S, Lertprasertsuke N, Srisawat U, Thuppia A, Ngamjariyawat A, Suwanlikhid N, Jaijoy K. Acute and subchronic toxicity study of the water extract from Tiliacoratriandra (Colebr.) Diels in rats. SJST. 2008;30 (5):611-9.

Righi N, Boumerfeg S, Deghima A, Fernandes PAR, Coelho E, Baali F, Cardoso SM, Coimbra MA, Baghiani A. Phenolic profile, safety assessment, and anti-inflammatory activity of Salvia verbenaca L. J Ethnopharmacol. 2021;272:113940. https://doi.org/10.1016/j.jep.2021.113940

Nouioura G, Tourabi M, Tahraoui A, El-yagoubi K, Maache S, Elfatemi H, Lyoussi B, Derwich EH. Assessment of the acute and subacute toxicity of the aqueous extract of Moroccan Ferula communis fruit in a mouse model. SPJ. 2023;31(8):101701. https://doi.org/10.1016/j.jsps.2023.101701

Aouachria S, Boumerfeg S, Benslama A, Benbacha F, Guemmez T, Khennouf S, Arrar L, Baghiani A. Acute, sub-acute toxicity and antioxidant activities (in vitro and in vivo) of Reichardi apicroide crude extract. J Ethnopharmacol. 2017;208:105-16. https://doi.org/10.1016/j.jep.2017.06.028

Grande D, Gioia MI, Terlizzese P, Iacoviello M. Heart Failure and Kidney Disease. In: Islam MdS, editor. Heart Failure: From Research to Clinical Practice. Vol. 3.Springer International Publishing; 2017. p. 219-38. (Advances in Experimental Medicine and Biology; Vol. 1067)https://doi.org/10.1007/5584_2017_126

Ben Khedir S, Mzid M, Bardaa S, Moalla D, Sahnoun Z, Rebai T. In vivo evaluation of the anti-inflammatory effect of Pistacia lentiscus fruit oil and its effects on oxidative stress. eCAM. 2016;2016:1-12. https://doi.org/10.1155/2016/6108203

Rosa MD, Willoughby DA. Screens for anti-inflammatory drugs. J Pharm Pharmacol. 1971;23(4):297-8. https://doi.org/10.1111/j.2042-7158.1971.tb08661.x

Boussouf L, Boutennoune H, Kebieche M, Adjeroud N, Al-Qaoud K, Madani K. Anti-inflammatory, analgesic and antioxidant effects of phenolic compound from Algerian Mentha rotundifolia L. leaves on experimental animals. S Afr J Bot. 2017;113:77-83. https://doi.org/10.1016/j.sajb.2017.07.003

Karbab A, Mokhnache K, Ouhida S, Charef N, Djabi F, Arrar L, Mubarak MS. Anti-inflammatory, analgesic activity, and toxicity of Pituranthosscoparius stem extract: An ethnopharmacological study in rat and mouse models. J Ethnopharmacol. 2020;258:112936. https://doi.org/10.1016/j.jep.2020.112936

Tunon M, Garcia-Mediavilla M, Sanchez-Campos S, Gonzalez-Gallego J. Potential of flavonoids as anti-inflammatory agents: Modulation of pro-inflammatory gene expression and signal transduction pathways. Curr Drug Metab. 2009;10(3):256-71. https://doi.org/10.2174/138920009787846369

Yahfoufi N, Alsadi N, Jambi M, Matar C. The Immunomodulatory and Anti-Inflammatory Role of Polyphenols. Nutrients. 2018;10(11):1618. https://doi.org/10.3390/nu10111618

Al Amin M, Chowdhury IA, Mahbub KMM, Sattar M, Shahriar M, Kuddus MR, Rashid MA.Anti-inflammatory and Analgesic Activities of Asteracantha longifoliaNees. Bangla Pharma J. 2012;15(2):171-6. https://doi.org/10.3329/bpj.v15i2.12586

Sobeh M, Rezq S, Cheurfa M, Abdelfattah MAO, Rashied RMH, El-Shazly AM, Yasri A, Wink M, Mahmoud MF. Thymus algeriensis and Thymus fontanesii: Chemical composition, in vivo anti-inflammatory, pain killing and antipyretic activities: A comprehensive comparison. Biomolecules. 2020;10(4):599. https://doi.org/10.3390/biom10040599

Aham EC, Okagu IU, Nwodo FOC, Ndefo JC. Analgesic and anti-inflammatory activities of ethanol extract of Hymenodictyonpachyantha K. Krause stem bark. Comp Clin Pathol. 2019;28(6):1779-90. https://doi.org/10.1007/s00580-019-03019-5

Meotti FC, Missau FC, Ferreira J, Pizzolatti MG, Mizuzaki C, Nogueira CW, Santos AR.Anti-allodynic property of flavonoid myricitrin in models of persistent inflammatory and neuropathic pain in mice. BiochemPharmacol. 2006;72(12):1707-13. https://doi.org/10.1016/j.bcp.2006.08.028

Ramesh M, Nageshwar Rao1 Y, Appa Rao AVN, Prabhakar MC, Seshagiri Rao1 C, Muralidhar N, Madahava Reddy B. Antinociceptive and anti-inflammatory activity of a flavonoid isolated from Carallumaattenuata. J Ethnopharmacol. 1998;62(1):63-6. https://doi.org/10.1016/S0378-8741(98)00048-8

Farahpour MR. Antioxidant activity, Antinociceptive and anti-inflammatory effects of Pot marigold hydroalcoholic extract on experimental animals. Inter J Pharm Tech Res. 2014;6(5):1640-6.65

Khouya T, Ramchoun M, Hmidani A, Bouhlali EDT, Amrani S, Alem C. Phytochemical analysis and bioactivity evaluation of Moroccan Thymus atlanticus (Ball) fractions. Sci Afr. 2021;11:e00716. https://doi.org/10.1016/j.sciaf.2021.e00716

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Published

2024-07-10

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Makhlouf Y, Bouaziz A, Benazi N, Djidel S, Bentahar A, Barghout N, Khennouf S, Dahamna S. Assessment of antioxidant and antiinflammatory activities and acute toxicity of the aqueous extract from a mixture of leaves and flowers of Anabasis articulata (Forssk.) Moq. Arch Biol Sci [Internet]. 2024Jul.10 [cited 2024Dec.3];76(2):161-74. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/9580

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