COMPARATIVE PHYTOCHEMICAL PROFILING AND EFFECTS OF NERIUM OLEANDER EXTRACTS ON THE ACTIVITIES OF MURINE PERITONEAL MACROPHAGES

Authors

  • Priyankar Dey Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri 734013, West Bengal
  • Tapas Kumar Chaudhuri Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri 734013, West Bengal

Abstract

Nerium oleander is a medicinal plant. Apart from its ethnopharmacological uses, pharmacognostic studies have revealed several of its bioactivities. Previously we demonstrated that the phenolic and flavonoid rich extracts of oleander leaf, stem and root possess potent antioxidant and free radical scavenging activities. Moreover, the leaf extract actively modulates the Th1/Th2 cytokine balance and exerts anti-inflammatory activities on murine splenic lymphocytes. Therefore, the present study was designed to evaluate the effect of oleander leaf, stem and root extracts on phagocytosis and the free radical-related activities of murine peritoneal macrophages. In addition, phytochemical profiling was performed using gas chromatography-mass spectrometry (GC-MS). The results demonstrated that the increase in phagocytosis and decrease in myeloperoxidase (MPO) were in the order of leaf>root>stem. The inhibition of cell adhesion, nitric oxide (NO) and elevation of respiratory burst activity was in the order of leaf>stem>root. However, the bioactivities of the leaf extract were much high than those of the stem and root extracts. Phytochemical analysis also revealed the presence of several bioactive constituents in oleander extracts. Therefore, the present study demonstrated that oleander possesses the capacity to modulate macrophage activities and the bioactivities are attributed to the numerous phytochemicals identified in oleander extracts.

 

Key words: GC-MS; immunomodulatory; macrophage; Nerium oleander; nitric oxide; phagocytosis

 

Received: August 16, 2015; Revised: September 28, 2015; Accepted: September 28, 2015; Published online: April 27, 2016

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References

Dey P, Chaudhuri TK. Pharmacological aspects of Nerium indicum Mill.: a comprehensive review. Pharmacog Rev. 2014;8(16):156-62.

Dey P, Chaudhuri D, Chaudhuri TK, Mandal N. Comparative assessment of the antioxidant activity and free radical scavenging potential of different parts of Nerium indicum. Int J Phytomed. 2012;4(1):54-69.

Dey P, Chaudhuri TK. Immunomodulatory activity of Nerium indicum through inhibition of nitric oxide and cyclooxygenase activity and modulation of TH1/TH2 cytokine balance in murine splenic lymphocytes. Cytotechnology. 2015;DOI: 10.1007/s10616-014-9826-9.

OECDiLibrary. Test No. 423: Acute Oral toxicity - Acute Toxic Class Method. OECD Pub; 2002. 14 p. (OECD guidelines for the testing of chemicals, Section 4: Health effects).

Gonda R, Tomoda M, Shimizu N, Kanari M. Characterization of an acidic polysaccharide from the seeds of Malva verticillata stimulating the phagocytic capacity of the RES. Planta Med. 1990;56(1):73-6.

Chakraborty AK, Chakravarty AK. Antibody-mediated immune response in the bat, Pteropus giganteus. Dev Comp Immunol. 1984;8(2):415-23.

Jadeja RN, Thounaojam MC, Jain M, Devkar RV, Ramachandran AV. Clerodendron glandulosum Coleb leaf extract attenuates in vitro macrophage differentiation and expression of VCAM-1 and P-selectin in thoracic aorta of atherogenic diet fed rats. Immunopharmacol Immunotoxicol. 2012;34(3):443-53.

Fortier AH, Falk LA. Isolation of murine macrophages. Curr Protoc Immunol. 2001; Chapter 14:Unit 14.1.

Fujiki K, Yano T. Effect of sodium alginate on the non-specific defense system of the common carp (Cyprinus caprio L.). Fish Shellfish Immun. 1997;7(6):417-27.

Lin TH, Rosales C, Mondal K, Bolen JB, Haskill S, Juliano RL. Integrin mediated tyrosine phosphorylation and cytokine message induction in monocytic cells. A possible signalling role for the Syk tyrosine kinase. J Biol Chem. 1995;270(27):16189-2.

Cook MT, Hayball PJ, Hutchinson W, Nowak B, Hayball JD. The efficacy of a commercial beta-glucan preparation, EcoActiva, on stimulating respiratory burst activity of head-kidney macrophages from pink snapper (Pagrus auratus), Sparidae. Fish Shellfish Immun. 2001;11(8):661-72.

Sengupta M, Sharma GD, Chakraborty B. Effect of aqueous extract of Tinospora cordifolia on functions of peritoneal macrophages isolated from CCl4 intoxicated male albino mice. BMC Complement Altern Med. 2011;11:102.

Hibbs JB Jr, Taintor RR, Vavrin Z, Rachlin EM. Nitric oxide: a cytotoxic activated macrophage effector molecule. Biochem Biophys Res Commun. 1988;157(1):87-94.

Akhtar T, Sheikh N, Abbasi MH. Clinical and pathological features of Nerium oleander extract toxicosis in wistar rats. BMC Res Notes. 2014;7:947.

Liu G, Xia XP, Gong SL, Zhao Y. The macrophage heterogeneity: difference between mouse peritoneal exudate and splenic F4/80+ macrophages. J Cell Physiol. 2006;209(2):341-52.

Muller BM, Rosskopf F, Paper DH, Kraus J, Franz G. Polysaccharides from Nerium oleander: structure and biological activity. Pharmazie. 1991;46(9):657-63.

Bor NM, Dereagzi H, Ibrahimoglu Z, Karabiyikoglu A, Ugurbas SH. Promotion of phagocytosis by Nerium oleander extract. J Islamic Acad Sci. 1988;1(2):141-2.

Hasui M, Hirabayashi Y, Hattori K, Kobayashi Y. Increased phagocytic activity of polymorphonuclear leukocytes of chronic granulomatous disease as determined with flow cytometric assay. J Lab Clin Med. 1991;117(4):291-8.

Stendahl O, Coble BI, Dahlgren C, Hed J, Molin L. Myeloperoxidase modulates the phagocytic activity of polymorphonuclear neutrophil leukocytes. Studies with cells from a myeloperoxidase-deficient patient. J Clin Invest. 1984;73(2):366-73.

Gerber CE, Kiifi S, Zipfel M, Niethammer D, Bruchelt G. Phagocytic Activity and Oxidative Burst of Granulocytes in Persons with Myeloperoxidase Deficiency. Eur J Clin Chem Clin Biochem. 1996;34(11):901-8.

O’Shea JJ, Brown EJ, Seligman BE, Metcalf JA, Frank MM, Gallin JI. Evidence for distinct intracellular pools of receptors for C3b and C3bi in human neutrophils. J Immunol. 1985;134(4):2580-7.

Middleton J, Patterson AM, Gardner L, Schmutz C, Ashton BA. Leukocyte extravasation: chemokine transport and presentation by the endothelium. Blood. 2002;100(12):3853-60.

Carveth HJ, Bohnsack JF, McIntyre TM, Baggiolini M, Prescott SM, Zimmerman GA. Neutrophil activating factor (NAF) induces polymorphonuclear leukocyte adherence to endothelial cells and to subendothelial matrix proteins. Biochem Biophys Res Commun. 1989;162(1):387-93.

Detmers PA, Lo SK, Olsen-Egbert E, Walz A, Baggiolini M, Cohn ZA. Neutrophil-activating protein interleukin 8 stimulates the binding activity of the leukocyte adhesion receptor CD11b/CD18 on human neutrophils. J Exp Med. 1990;171(4):1155-62.

Thounaojam MC, Jadeja RN, Salunke SP, Devkar RV, Ramachandran AV. Sida rhomboidea Roxb aqueous extract down-regulates in vivo expression of vascular cell adhesion molecules in atherogenic rats and inhibits in vitro macrophage differentiation and foam cell formation. Immunopharmacol Immunotoxicol. 2012;34(5):832-43.

Kim HJ, McLean D, Pyee J, Kim J, Park H. Extract from Acanthopanax senticosus prevents LPS-induced monocytic cell adhesion via suppression of LFA-1 and Mac-1. Can J Physiol Pharmacol. 2014;92(4):278-84.

Dong Q, Liu X, Yao J, Dong X, Ma C, Xu Y, Fang J, Ding K. Structural characterization of a pectic polysaccharide from Nerium indicum flowers. Phytochemistry. 2010;71(11-12):1430-7.

Sreenivasan Y, Sarkar A, Manna SK. Oleandrin suppresses activation of nuclear transcription factor-kappa B and activator protein-1 and potentiates apoptosis induced by ceramide. Biochem Pharmacol. 2003;66(11):2223-39.

Dey P, Chaudhuri TK. Anti-inflammatory activity of Nerium indicum by inhibition of prostaglandin E2 in murine splenic lymphocytes. Indian J Pharmacol. 2015;47(4):447-50.

Chiang LC, Ng LT, Chiang W, Chang MY, Lin CC. Immunomodulatory activities of flavonoids, monoterpenoids, triterpenoids, iridoid glycosides and phenolic compounds of Plantago species. Planta Med. 2003;69(7):600-4.

Durga.M, Nathiya. S, Devasena.T. Immunomodulatory and antioxidant actions of dietary flavonoids. Int J Pharm Pharm Sci. 2014;6(2):50-6.

Harmatha J, Zídek Z, Kmonickova E, Smidrkal J. Immunobiological properties of selected natural and chemically modified phenylpropanoids. Interdiscip Toxicol. 2011;4(1):5-10.

Tunon MJ, Garcia-Mediavilla MV, Sanchez-Campos S, Gonzalez-Gallego J. Potential of flavonoids as anti-inflammatory agents: modulation of pro-inflammatory gene expression and signal transduction pathways. Curr Drug Metab. 2009;10(3):256-71.

Kumar S, Pandey AK.. Chemistry and biological activities of flavonoids: an overview. ScientificWorldJournal. 2013;29:162750.

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Published

2016-09-05

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1.
Dey P, Chaudhuri TK. COMPARATIVE PHYTOCHEMICAL PROFILING AND EFFECTS OF NERIUM OLEANDER EXTRACTS ON THE ACTIVITIES OF MURINE PERITONEAL MACROPHAGES. Arch Biol Sci [Internet]. 2016Sep.5 [cited 2024Apr.25];68(3):515-31. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/970

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Vol. 68 No. 3 (2016)

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Articles

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