Phytochemical profile and hepatoprotective effects of Crotalaria vialattei against antituberculosis drug-induced liver injury

Authors

  • Kaouther Zouioueche 1. Research Unit, Valorization of Natural Resources, Bioactive Molecules, and Physicochemical and Biological Analyses (VARENBIOMOL), University of Constantine 1 Frères Mentouri, Ain El-Bey Road, 25000, Constantine, Algeria; 2. Department of Animal Biology, Faculty of Natural and Life Sciences, University of Constantine 1 Frères Mentouri, Aïn El Bey Road, 25000, Constantine, Algeria https://orcid.org/0009-0002-9108-4424
  • Meriem Laraba 1. Research Unit, Valorization of Natural Resources, Bioactive Molecules, and Physicochemical and Biological Analyses (VARENBIOMOL), University of Constantine 1 Frères Mentouri, Ain El-Bey Road, 25000, Constantine, Algeria; 2. Department of Animal Biology, Faculty of Natural and Life Sciences, University of Constantine 1 Frères Mentouri, Aïn El Bey Road, 25000, Constantine, Algeria https://orcid.org/0000-0003-2069-6570
  • Afifa Bensegueni Department of Anatomic Pathology, Faculty of Medicine, University Salah Boubnider Constantine 3, Constantine, Algeria https://orcid.org/0009-0007-1977-3600
  • Iman Ramli Applied Biochemisty laboratory, University of Constantine 1 Frères Mentouri, Ain El Bey Road, 25000, Constantine, Algeria https://orcid.org/0000-0002-3364-967X
  • Leila Hammoud Research Unit, Valorization of Natural Resources, Bioactive Molecules, and Physicochemical and Biological Analyses (VARENBIOMOL), University of Constantine 1 Frères Mentouri, Ain El-Bey Road, 25000, Constantine, Algeria https://orcid.org/0009-0007-1586-1785
  • Ramazan Erenler Department of Chemistry, Faculty of Arts and Sciences, Tokat Gaziosmanpasa University, 60240 Tokat, Türkiye https://orcid.org/0000-0002-0505-3190
  • İlyas Yıldız Department of Molecular Biology and Genetics, Department of Biotechnology, Institute of Science, Tokat Gaziosmanpaşa University, 60240 Tokat, Türkiye https://orcid.org/0000-0003-1254-1069
  • Imene Messaoud Nacer Laboratory of Biotechnology, Environment and Health, University of Blida 1, Blida, Algeria https://orcid.org/0009-0009-0028-8685
  • Aya Berkane 1. Research Unit, Valorization of Natural Resources, Bioactive Molecules, and Physicochemical and Biological Analyses (VARENBIOMOL), University of Constantine 1 Frères Mentouri, Ain El-Bey Road, 25000, Constantine, Algeria; 2. Department of Biochemistry and Cellular and Molecular Biology, Faculty of Natural and Life Sciences, University of Constantine 1 Frères Mentouri, Aïn El Bey Road, 25000, Constantine, Algeria https://orcid.org/0009-0003-7967-658X
  • Chawki Bensouici Biotechnology Research Center (CRBT), Ali Mendjeli New Town UV 03, Constantine 25000, Algeria https://orcid.org/0000-0003-4612-4642
  • Fadila Benayache Research Unit, Valorization of Natural Resources, Bioactive Molecules, and Physicochemical and Biological Analyses (VARENBIOMOL), University of Constantine 1 Frères Mentouri, Ain El-Bey Road, 25000, Constantine, Algeria https://orcid.org/0000-0002-2282-6343
  • Samir Benayache Research Unit, Valorization of Natural Resources, Bioactive Molecules, and Physicochemical and Biological Analyses (VARENBIOMOL), University of Constantine 1 Frères Mentouri, Ain El-Bey Road, 25000, Constantine, Algeria https://orcid.org/0000-0003-2298-2466
  • Djamila Zama 1. Research Unit, Valorization of Natural Resources, Bioactive Molecules, and Physicochemical and Biological Analyses (VARENBIOMOL), University of Constantine 1 Frères Mentouri, Ain El-Bey Road, 25000, Constantine, Algeria; 2. Department of Animal Biology, Faculty of Natural and Life Sciences, University of Constantine 1 Frères Mentouri, Aïn El Bey Road, 25000, Constantine, Algeria https://orcid.org/0000-0002-4312-7701

DOI:

https://doi.org/10.2298/ABS260110001Z

Keywords:

Crotalaria vialattei, RHZ, polyphenols, antioxidant activity, hepatoprotective effect

Abstract

Paper description:

  • Crotalaria vialattei, is an endemic Algerian plant whose phytochemical composition and biological properties remain poorly documented.
  • The n-butanol extract of vialattei was characterized by LC–MS/MS and evaluated for its antioxidant activity and hepatoprotective effects in a rat model of antituberculosis drug-induced hepatotoxicity.
  • The extract exhibited a phenolic-rich profile, measurable antioxidant activity, and significant protective effects against RHZ-induced hepatic alterations.
  • These findings provide new experimental evidence supporting the potential relevance of vialattei as a source of bioactive compounds with hepatoprotective activity.

Abstract: Tuberculosis is a global public health concern, and its treatment is frequently associated with hepatotoxic side effects. Phytotherapy represents a promising complementary approach. This study aimed to characterize the phenolic profile of the n-butanol extract of Crotalaria vialattei (BCV), evaluate its antioxidant capacity, and assess its hepatoprotective effects against liver damage induced by a fixed-dose antituberculosis drug combination containing rifampin, isoniazid, and pyrazinamide (RHZ). Phytochemical analysis revealed a phenolic-rich extract, identifying 17 polyphenolic compounds. BCV exhibited measurable antioxidant activity in vitro. In vivo, oral administration of RHZ (rifampin 150 mg/kg, isoniazid 75 mg/kg, and pyrazinamide 400 mg/kg) induced marked alterations in hepatic biochemical markers, lipid profile, oxidative status, and liver histoarchitecture. BCV treatment significantly attenuated these changes by improving liver enzyme activities, restoring oxidative balance, and preserving liver architecture. Overall, the BCV extract demonstrates antioxidant-associated hepatoprotective potential against RHZ-induced liver injury and may represent a promising complementary strategy to reduce antituberculosis drug-related hepatotoxicity.

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Published

2026-04-09

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1.
Zouioueche K, Laraba M, Bensegueni A, Ramli I, Hammoud L, Erenler R, Yıldız İlyas, Messaoud Nacer I, Berkane A, Bensouici C, Benayache F, Benayache S, Zama D. Phytochemical profile and hepatoprotective effects of Crotalaria vialattei against antituberculosis drug-induced liver injury. Arch Biol Sci [Internet]. 2026Apr.9 [cited 2026Apr.10];78(1):45-56. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/12041

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Vol. 78 No. 1 (2026)

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Copyright (c) 2026 KAOUTHER ZOUIOUECHE, Meriem Laraba, Afifa Bensegueni, Iman Ramli, Leila Hammoud, Ramazan Erenler, İlyas Yıldız, Imene Messaoud Nacer, Aya Berkane, Chawki Bensouici, Fadila Benayache, Samir Benayache, Djamila Zama

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