ISOLATION AND IDENTIFICATION OF BACILLUS SPP. FROM COMPOST MATERIAL, COMPOST AND MUSHROOM CASING SOIL ACTIVE AGAINST TRICHODERMA SPP.

Authors

  • Olja Stanojević Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade
  • Svetlana Milijašević-Marčić Institute of Pesticides and Environmental Protection, Laboratory of Applied Phytopathology, Banatska 31b, 11080 Belgrade
  • Ivana Potočnik Institute of Pesticides and Environmental Protection, Laboratory of Applied Phytopathology, Banatska 31b, 11080 Belgrade
  • Miloš Stepanović Institute of Pesticides and Environmental Protection, Laboratory of Applied Phytopathology, Banatska 31b, 11080 Belgrade
  • Ivica Dimkić Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade
  • Slaviša Stanković Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade
  • Tanja Berić Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade

Abstract

The isolation of bacteria was carried out from samples of straw and chicken manure, compost at various stages of the composting process and casing soil used for growing button mushrooms. A preliminary screening of 108 bacterial isolates for antagonistic activity against Trichoderma aggressivum f. europaeum showed that 23 tested isolates inhibited mycelial growth of the pathogenic fungus. Further screening with four indicator isolates of fungi revealed that all 23 bacterial isolates inhibited the growth of T. aggressivum f. europaeum, T. harzianum and T. koningii, while only 13 isolates inhibited the growth of T. atroviride. T. aggressivum f. europaeum proved to be the most sensitive, with many bacterial isolates generating a high percentage of growth inhibition. Only two bacterial isolates (B-129 and B-268) were successful in inhibiting the growth of all 4 tested pathogens. All 23 bacterial isolates were characterized as Gram-positive and catalase-positive and were subjected to molecular identification based on the partial sequence, the hypervariant region of the 16S rDNA. It was shown that the obtained bacterial strains belong to Bacillus subtilis, B. amyloliquefaciens, B. licheniformis and B. pumilus species.

DOI: 10.2298/ABS151104073S

Key words: Agaricus bisporus; Bacillus; biocontrol; green mold; hypervariant region

Received: November 4, 2015; Revised: December 25, 2015; Accepted: January 21, 2016; Published online: August 9, 2016

How to cite this article: Stanojević O, Milijašević-Marčić S, Potočnik I, Stepanović M, Dimkić I, Stanković S, Berić T. Isolation and identification of Bacillus spp. from compost material, compost and mushroom casing soil active against Trichoderma spp.. Arch Biol Sci. 2016;68(4):845-52.

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Published

2016-11-25

How to Cite

1.
Stanojević O, Milijašević-Marčić S, Potočnik I, Stepanović M, Dimkić I, Stanković S, Berić T. ISOLATION AND IDENTIFICATION OF BACILLUS SPP. FROM COMPOST MATERIAL, COMPOST AND MUSHROOM CASING SOIL ACTIVE AGAINST TRICHODERMA SPP. Arch Biol Sci [Internet]. 2016Nov.25 [cited 2022May29];68(4):845-52. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1258

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