Identification and antibiotic resistance of Bacillus spp. isolates from natural samples

Authors

  • Tanja Berić University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade http://orcid.org/0000-0002-4860-2225
  • Marjan Biočanin 1. University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade, Serbia; 2. Swiss Federal Institute of Technology in Lausanne, School of Life Sciences, Institute for Bioengineering, Laboratory of Systems Biology and Genetics, Lausanne, Switzerland
  • Slaviša Stanković University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Ivica Dimkić University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Tamara Janakiev University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Đorđe Fira University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade
  • Jelena Lozo University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade

Keywords:

Bacillus, 5' hypervariable 16S rRNA, antibiotic resistance, erythromycin-resistance (erm)

Abstract

Paper description:

  • Non-pathogenic bacteria from the environment as a source of antibiotic resistance determinants are recognized.
  • Identification of 33 Bacillus spp. isolates from different natural samples from Serbia was performed by sequencing the 5' hypervariable section of the 16S rRNA gene. Eight species was determined. This is the first report about antibiotic resistance of natural isolates of Bacillus spp. from Serbian soil, manure and straw.
  • The potential for the presence and spread of resistance determinants in the soil and similar ecosystems exists, and monitoring of antibiotic resistance genes in nonpathogenic Bacillus strains from the environment is advised.


Abstract: Identification of 33 Bacillus spp. isolates from different environmental samples collected from the territory of Serbia was performed by sequencing of the 5'-hypervariable section of 16S rRNA gene. Eight species were identified within four phylogenetic groups: B. pumilus, B. megaterium, B. subtilis and B. cereus. Determination of their antibiotic resistance was performed using the minimum inhibitory concentration (MIC) assay. We found that just one isolate was resistant to gentamicin, 9 were resistant to clindamycin and all were resistant to vancomycin. Based on the profile of resistance, the isolates were categorized into 4 categories. In silico analysis of the erythromycin-resistance (erm) gene for clindamycin resistance showed their distribution between related and nonrelated soil and human isolates including different species of Bacillus genera. This finding indicates that Bacillus spp. from the environment could be a source of resistance to clindamycin. The potential for the presence and spread of resistance determinants in the soil and similar ecosystems exists so that monitoring of antibiotic resistance genes in nonpathogenic Bacillus strains from the environment is advised.

https://doi.org/10.2298/ABS180302019B

Received: March 2, 2018; Revised: April 10, 2018; Accepted: April 23, 2018; Published online: May 14, 2018

How to cite this article: Berić T, Biočanin M, Stanković S, Dimkić I, Janakiev T, Fira Đ, Lozo J. Identification and antibiotic resistance of Bacillus spp. isolates from natural samples. Arch Biol Sci. 2018;70(3):…

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Published

2018-08-20

How to Cite

1.
Berić T, Biočanin M, Stanković S, Dimkić I, Janakiev T, Fira Đorđe, Lozo J. Identification and antibiotic resistance of Bacillus spp. isolates from natural samples. Arch Biol Sci [Internet]. 2018Aug.20 [cited 2022May29];70(3):581-8. Available from: https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2720

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